################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BcgI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 469 CCGC CCGC(-3/-1) AlwNI 368 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG BamHI 344 GGATCC G^GATCC BbvCI 595 CCTCAGC CCTCAGC(-5/-2) BciVI 211 GTATCC GTATCC(6/5) BclI 738 TGATCA T^GATCA BfaI 356 CTAG C^TAG BlpI 514 GCTCAGC GC^T[ACGT]AGC BmyI 68 GTGCCC G[AGT]GC[ACT]^C Bpu1102I 514 GCTCAGC GC^T[ACGT]AGC BsaJI 472 CCTTGG C^C[ACGT][ACGT]GG BseRI 692 CTCCTC GAGGAG(10/8) BsmAI 702 GTCTC GTCTC(1/5) BsmFI 463 GTCCC GGGAC(10/14) BsmI 839 GCATTC GAATGC(1/-1) Bsp1286I 68 GTGCCC G[AGT]GC[ACT]^C BstYI 344 GGATCC [GA]^GATC[CT] CelII 514 GCTCAGC GC^T[ACGT]AGC CfoI 799 GCGC GCG^C EarI 609 CTCTTC CTCTTC(1/4) EcoRV 404 GATATC GAT^ATC EcoT14I 472 CCTTGG C^C[AT][AT]GG FbaI 738 TGATCA T^GATCA HhaI 799 GCGC GCG^C HinP1I 799 GCGC G^CGC HinfI 10 GATTC G^A[ACGT]TC Ksp632I 609 CTCTTC CTCTTC(1/4) MaeI 356 CTAG C^TAG MaeII 483 ACGT A^CGT MflI 344 GGATCC [GA]^GATC[CT] MspA1I 371 CAGCTG C[AC]G^C[GT]G NlaIII 742 CATG CATG^ PvuII 371 CAGCTG CAG^CTG SexAI 764 ACCAGGT A^CC[AT]GGT StyI 472 CCTTGG C^C[AT][AT]GG TfiI 10 GATTC G^A[AT]TC XhoII 344 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 151 AAATTT [GA]^AATT[CT] 263 AAATTT ApoI 151 AAATTT [GA]^AATT[CT] 263 AAATTT AvaII 316 GGTCC G^G[AT]CC 462 GGTCC BbvI 370 GCAGC GCAGC(8/12) 810 GCAGC BpmI 446 CTCCAG CTGGAG(16/14) 206 CTGGAG BspMI 534 ACCTGC ACCTGC(4/8) 629 ACCTGC BstXI 466 CCACCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 571 CCAGGACAGTGG Cac8I 366 GCCAGC GC[ACGT]^[ACGT]GC 795 GCCTGC Cfr13I 316 GGTCC G^G[ACGT]CC 462 GGTCC Fnu4HI 370 GCAGC GC^[ACGT]GC 810 GCAGC HaeIII 476 GGCC GG^CC 552 GGCC HphI 40 TCACC GGTGA(8/7) 762 TCACC ItaI 370 GCAGC GC^[ACGT]GC 810 GCAGC MslI 421 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 786 CATCACTATG NlaIV 344 GGATCC GG[ACGT]^[ACGT]CC 462 GGTCCC PflMI 73 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 367 CCAGCAGCTGG Sau96I 316 GGTCC G^G[ACGT]CC 462 GGTCC TseI 370 GCAGC G^C[AT]GC 810 GCAGC Tsp45I 39 GTCAC ^GT[GC]AC 232 GTCAC Van91I 73 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 367 CCAGCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AluI AlwI BsiYI BslI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII FokI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII Sau3AI ScrFI SfaNI Tru9I Tsp509I TspRI