################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluNI MroI MscI MspA1I MunI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccII 94 CGCG CG^CG AflIII 93 ACGCGT A^C[GA][CT]GT AluI 546 AGCT AG^CT BbvI 155 GCAGC GCAGC(8/12) BfaI 548 CTAG C^TAG BmyI 377 GGGCAC G[AGT]GC[ACT]^C BpmI 82 CTGGAG CTGGAG(16/14) Bsp1286I 377 GGGCAC G[AGT]GC[ACT]^C BsrDI 506 GCAATG GCAATG(2/0) BstUI 94 CGCG CG^CG Cac8I 496 GCTGGC GC[ACGT]^[ACGT]GC DraII 587 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 380 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 587 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 233 ATGCAT ATGCA^T Fnu4HI 155 GCAGC GC^[ACGT]GC HgaI 92 GACGC GACGC(5/10) HinfI 469 GAATC G^A[ACGT]TC HphI 280 GGTGA GGTGA(8/7) ItaI 155 GCAGC GC^[ACGT]GC MaeI 548 CTAG C^TAG MaeIII 97 GTGAC ^GT[ACGT]AC MboII 393 GAAGA GAAGA(8/7) MluI 93 ACGCGT A^CGCGT MslI 236 CATCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 94 CGCG CG^CG NsiI 233 ATGCAT ATGCA^T NspI 492 ACATGC [GA]CATG^[CT] PpuMI 587 GGGACCC [GA]G^G[AT]CC[CT] TaqI 357 TCGA T^CGA TfiI 469 GAATC G^A[AT]TC TseI 155 GCAGC G^C[AT]GC Tsp45I 97 GTGAC ^GT[GC]AC TthHB8I 357 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 301 GAATTT [GA]^AATT[CT] 330 AAATTT AfaI 422 GTAC GT^AC 428 GTAC ApoI 301 GAATTT [GA]^AATT[CT] 330 AAATTT AvaII 436 GGACC G^G[AT]CC 588 GGACC BcnI 46 CCCGG CC^[GC]GG 513 CCGGG BsmFI 516 GGGAC GGGAC(10/14) 587 GGGAC BstNI 81 CCTGG CC^[AT]GG 202 CCAGG DpnII 128 GATC ^GATC 183 GATC EaeI 114 CGGCCA [CT]^GGCC[GA] 510 TGGCCG EcoRII 81 CCTGG ^CC[AT]GG 202 CCAGG HapII 47 CCGG C^CGG 513 CCGG HincII 10 GTCAAC GT[CT]^[GA]AC 219 GTTGAC HindII 10 GTCAAC GT[CT]^[GA]AC 219 GTTGAC HpaII 47 CCGG C^CGG 513 CCGG MboI 128 GATC ^GATC 183 GATC MspI 47 CCGG C^CGG 513 CCGG MvaI 81 CCTGG CC^[AT]GG 202 CCAGG NciI 46 CCCGG CC^[GC]GG 513 CCGGG NdeII 128 GATC ^GATC 183 GATC NlaIV 587 GGGACC GG[ACGT]^[ACGT]CC 588 GGACCC RsaI 422 GTAC GT^AC 428 GTAC Sau3AI 128 GATC ^GATC 183 GATC SfaNI 158 GCATC GCATC(5/9) 235 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### BsaJI BsrI BssKI BstF5I Cfr13I FokI HaeIII MnlI MseI NlaIII Sau96I ScrFI Tru9I Tsp509I TspRI