(Circular) (Six-base) MAPSORT of: pFN21AE2546.seq Check: 7043 from: 1 to: 8335 pFN21AE2546 8335 bp With 182 enzymes: SgfI * October 24, 2014 11:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6145 278 Size: 53 83 186 936 4609 2468 Fragments arranged by size: 4609 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3401 4324 5160 5678 1937 Size: 1464 923 836 518 4594 Fragments arranged by size: 4594 1464 923 836 518 Acc65I G'GTAC_C Cuts at: 5656 5656 Size: 8335 AclI AA'CG_TT Cuts at: 1563 6462 6835 1563 Size: 4899 373 3063 Fragments arranged by size: 4899 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2183 2577 2936 3237 3299 4332 4395 5366 Size: 551 394 359 301 62 1033 63 971 Cuts at: 5366 6398 7782 8050 1632 Size: 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1033 1032 971 551 394 359 301 268 63 62 AflII C'TTAA_G Cuts at: 820 1017 4003 820 Size: 197 2986 5152 Fragments arranged by size: 5152 2986 197 AflIII A'CryG_T Cuts at: 1203 3096 5038 7234 8211 1203 Size: 1893 1942 2196 977 1327 Fragments arranged by size: 2196 1942 1893 1327 977 AgeI A'CCGG_T Cuts at: 7166 7166 Size: 8335 AhdI GACnn_n'nnGTC Cuts at: 3397 7064 3397 Size: 3667 4668 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3134 5264 5343 1184 Size: 1950 2130 79 4176 Fragments arranged by size: 4176 2130 1950 79 AlwNI CAG_nnn'CTG Cuts at: 5336 6078 7650 5336 Size: 742 1572 6021 Fragments arranged by size: 6021 1572 742 ApaI G_GGCC'C Cuts at: 2087 2087 Size: 8335 ApaLI G'TGCA_C Cuts at: 6392 7548 6392 Size: 1156 7179 ApoI r'AATT_y Cuts at: 1493 2170 2285 2454 2983 3250 4388 4854 5135 Size: 677 115 169 529 267 1138 466 281 Cuts at: 5135 5204 5644 5870 6022 8131 1493 Size: 69 440 226 152 2109 1697 Fragments arranged by size: 2109 1697 1138 677 529 466 440 281 267 226 169 152 115 69 AseI AT'TA_AT Cuts at: 160 1032 2232 3030 6889 160 Size: 872 1200 798 3859 1606 Fragments arranged by size: 3859 1606 1200 872 798 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8335 AvaI C'yCGr_G Cuts at: 1870 1943 3191 5660 1870 Size: 73 1248 2469 4545 Fragments arranged by size: 4545 2469 1248 73 BamHI G'GATC_C Cuts at: 5665 6032 5665 Size: 367 7968 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4867 5656 7111 618 Size: 325 217 627 3080 789 1455 1842 Fragments arranged by size: 3080 1842 1455 789 627 325 217 BanII G_rGCy'C Cuts at: 1391 2087 5654 1391 Size: 696 3567 4072 Fragments arranged by size: 4072 3567 696 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3042 3480 4566 928 Size: 971 1143 438 1086 4697 Fragments arranged by size: 4697 1143 1086 971 438 BbvCI CC'TCA_GC Cuts at: 2689 4935 5439 2689 Size: 2246 504 5585 Fragments arranged by size: 5585 2246 504 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6526 6560 1903 Size: 34 4589 34 3678 Fragments arranged by size: 4589 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6227 7437 6227 Size: 1210 7125 BclI T'GATC_A Cuts at: 1548 1548 Size: 8335 BfrBI ATG'CAT Cuts at: 2918 2918 Size: 8335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6946 136 Size: 107 122 71 631 605 5274 1525 Fragments arranged by size: 5274 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2158 3302 3448 8330 2158 Size: 1144 146 4882 2163 Fragments arranged by size: 4882 2163 1144 146 BlpI GC'TnA_GC Cuts at: 2367 5067 5752 2367 Size: 2700 685 4950 Fragments arranged by size: 4950 2700 685 Bme1580I G_kGCm'C Cuts at: 1790 2087 4777 6396 7552 1790 Size: 297 2690 1619 1156 2573 Fragments arranged by size: 2690 2573 1619 1156 297 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2760 6066 7024 Size: 416 53 83 399 24 1343 3306 958 Cuts at: 7024 442 Size: 1753 Fragments arranged by size: 3306 1753 1343 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3586 5202 6995 1239 Size: 2347 1616 1793 2579 Fragments arranged by size: 2579 2347 1793 1616 Bpu10I CC'TnA_GC Cuts at: 2689 4935 5439 2689 Size: 2246 504 5585 Fragments arranged by size: 5585 2246 504 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2249 2868 6460 7325 7623 2249 Size: 619 3592 865 298 2961 Fragments arranged by size: 3592 2961 865 619 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3749 4703 6998 882 Size: 617 2250 954 2295 2219 Fragments arranged by size: 2295 2250 2219 954 617 BsaAI yAC'GTr Cuts at: 493 1204 6126 493 Size: 711 4922 2702 Fragments arranged by size: 4922 2702 711 BsaBI GATnn'nnATC Cuts at: 5516 8329 5516 Size: 2813 5522 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6142 6524 7159 275 Size: 53 83 186 936 4609 382 635 1451 Fragments arranged by size: 4609 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6768 7166 7440 7587 7998 6768 Size: 398 274 147 411 7105 Fragments arranged by size: 7105 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5200 5230 1178 Size: 30 165 30 3797 30 4283 Fragments arranged by size: 4283 3797 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2834 3567 5493 5496 1186 Size: 1648 733 1926 3 4025 Fragments arranged by size: 4025 1926 1648 733 3 BseYI C'CCAG_C Cuts at: 3893 5413 7538 8034 3893 Size: 1520 2125 496 4194 Fragments arranged by size: 4194 2125 1520 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 2878 1163 Size: 573 603 539 6620 Fragments arranged by size: 6620 603 573 539 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5785 6546 7574 1540 Size: 275 186 3784 761 1028 2301 Fragments arranged by size: 3784 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2395 6396 6481 7552 2395 Size: 4001 85 1071 3178 Fragments arranged by size: 4001 3178 1071 85 BsmI GAATG_Cn' Cuts at: 5846 5939 5846 Size: 93 8242 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2395 4777 5654 6396 6481 7552 Size: 399 297 308 2382 877 742 85 1071 Cuts at: 7552 1391 Size: 2174 Fragments arranged by size: 2382 2174 1071 877 742 399 308 297 85 BspEI T'CCGG_A Cuts at: 7998 7998 Size: 8335 BspHI T'CATG_A Cuts at: 4290 6224 7954 4290 Size: 1934 1730 4671 Fragments arranged by size: 4671 1934 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5690 844 Size: 4846 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6222 1394 Size: 429 4399 3507 Fragments arranged by size: 4399 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2859 6830 7004 65 Size: 1183 1611 3971 174 1396 Fragments arranged by size: 3971 1611 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6979 7166 1700 Size: 110 5169 187 2869 Fragments arranged by size: 5169 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8335 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8335 BssSI C'ACGA_G Cuts at: 6395 7407 6395 Size: 1012 7323 BstAPI GCAn_nnn'nTGC Cuts at: 794 5173 5257 5336 794 Size: 4379 84 79 3793 Fragments arranged by size: 4379 3793 84 79 BstBI TT'CG_AA Cuts at: 7139 7139 Size: 8335 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8335 BstXI CCAn_nnnn'nTGG Cuts at: 4977 4977 Size: 8335 BstYI r'GATC_y Cuts at: 1972 2158 2560 3302 3448 5665 6032 6417 6434 Size: 186 402 742 146 2217 367 385 17 Cuts at: 6434 7886 7972 7984 8330 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2217 1977 1452 742 402 385 367 346 186 146 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6844 Fragments arranged by size: 6844 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7596 Fragments arranged by size: 7596 683 56 BtsI GCAGTG_nn' Cuts at: 5588 5851 6645 6665 5588 Size: 263 794 20 7258 Fragments arranged by size: 7258 794 263 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3073 3108 529 Size: 35 2509 35 5756 Fragments arranged by size: 5756 2509 35 35 DraI TTT'AAA Cuts at: 4107 4362 5374 5639 4107 Size: 255 1012 265 6803 Fragments arranged by size: 6803 1012 265 255 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7342 809 Size: 507 6026 1802 Fragments arranged by size: 6026 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3320 5190 5782 Size: 54 1425 50 275 18 1490 1870 592 Cuts at: 5782 6671 8300 8 Size: 889 1629 43 Fragments arranged by size: 1870 1629 1490 1425 889 592 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5782 1537 Size: 275 3970 4090 Fragments arranged by size: 4090 3970 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 3213 5636 6265 1353 Size: 1167 693 2423 629 3423 Fragments arranged by size: 3423 2423 1167 693 629 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6922 7296 7442 387 Size: 1064 138 5333 374 146 1280 Fragments arranged by size: 5333 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2577 2936 3237 3299 3586 4332 Size: 393 551 394 359 301 62 287 746 Cuts at: 4332 4395 5202 5366 6398 6995 7782 8050 1239 Size: 63 807 164 1032 597 787 268 1524 Fragments arranged by size: 1524 1032 807 787 746 597 551 394 393 359 301 287 268 164 63 62 EcoO109I rG'GnC_Cy Cuts at: 2083 2084 4939 2083 Size: 1 2855 5479 Fragments arranged by size: 5479 2855 1 EcoRI G'AATT_C Cuts at: 5644 6022 5644 Size: 378 7957 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3457 3457 Size: 8335 FspI TGC'GCA Cuts at: 6841 6841 Size: 8335 HaeII r_GCGC'y Cuts at: 1678 7482 1678 Size: 5804 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2622 2654 2708 2740 3098 Size: 32 135 32 1050 32 54 32 358 Cuts at: 3098 3130 3383 3415 3584 3616 4243 4275 5458 Size: 32 253 32 169 32 627 32 1183 Cuts at: 5458 5490 6976 7008 7050 7082 1373 Size: 32 1486 32 42 32 2626 Fragments arranged by size: 2626 1486 1183 1050 627 358 253 169 135 54 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3226 5679 5925 669 Size: 988 281 1288 2453 246 3079 Fragments arranged by size: 3079 2453 1288 988 281 246 HindIII A'AGCT_T Cuts at: 748 4219 748 Size: 3471 4864 HpaI GTT'AAC Cuts at: 3226 5925 3226 Size: 2699 5636 KpnI G_GTAC'C Cuts at: 5660 5660 Size: 8335 MfeI C'AATT_G Cuts at: 4791 5934 4791 Size: 1143 7192 MluI A'CGCG_T Cuts at: 8211 8211 Size: 8335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2371 4160 7449 7633 1305 Size: 1066 1789 3289 184 2007 Fragments arranged by size: 3289 2007 1789 1066 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3322 5192 10 Size: 54 1425 343 1490 1870 3153 Fragments arranged by size: 3153 1870 1490 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3116 3482 5043 6293 6652 6811 518 Size: 648 1950 366 1561 1250 359 159 2042 Fragments arranged by size: 2042 1950 1561 1250 648 366 359 159 MspA1I CmG'CkG Cuts at: 1606 2693 5066 5222 5333 5342 5348 5360 5751 Size: 1087 2373 156 111 9 6 12 391 Cuts at: 5751 6428 7576 7821 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2373 2120 1148 1087 677 391 245 156 111 12 9 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6844 Fragments arranged by size: 6844 939 552 NdeI CA'TA_TG Cuts at: 387 2239 3825 387 Size: 1852 1586 4897 Fragments arranged by size: 4897 1852 1586 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8335 NotI GC'GGCC_GC Cuts at: 5782 5782 Size: 8335 NsiI A_TGCA'T Cuts at: 2920 2920 Size: 8335 NspI r_CATG'y Cuts at: 1132 1229 3100 5042 5188 5693 7238 1132 Size: 97 1871 1942 146 505 1545 2229 Fragments arranged by size: 2229 1942 1871 1545 505 146 97 PasI CC'CwG_GG Cuts at: 1348 2617 1348 Size: 1269 7066 PciI A'CATG_T Cuts at: 3096 5038 7234 3096 Size: 1942 2196 4197 Fragments arranged by size: 4197 2196 1942 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8335 PfoI T'CCnGG_A Cuts at: 2312 2395 2312 Size: 83 8252 PmeI GTTT'AAAC Cuts at: 5639 5639 Size: 8335 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6404 6436 7917 7949 6404 Size: 32 1481 32 6790 Fragments arranged by size: 6790 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8335 PsiI TTA'TAA Cuts at: 4989 5905 4989 Size: 916 7419 PspOMI G'GGCC_C Cuts at: 2083 2083 Size: 8335 PstI C_TGCA'G Cuts at: 830 2698 3943 5178 5253 5332 5341 5347 5353 Size: 1868 1245 1235 75 79 9 6 6 Cuts at: 5353 5365 5687 830 Size: 12 322 3478 Fragments arranged by size: 3478 1868 1245 1235 322 79 75 12 9 6 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8335 PvuII CAG'CTG Cuts at: 2693 5066 5222 5333 5342 5348 5360 2693 Size: 2373 156 111 9 6 12 5668 Fragments arranged by size: 5668 2373 156 111 12 9 6 SalI G'TCGA_C Cuts at: 1936 5677 1936 Size: 3741 4594 SbfI CC_TGCA'GG Cuts at: 5687 5687 Size: 8335 ScaI AGT'ACT Cuts at: 2201 4408 6583 8295 2201 Size: 2207 2175 1712 2241 Fragments arranged by size: 2241 2207 2175 1712 SfcI C'TryA_G Cuts at: 826 1045 2694 3306 3367 3516 3939 4201 5174 Size: 219 1649 612 61 149 423 262 973 Cuts at: 5174 5249 5328 5337 5343 5349 5361 5683 6818 Size: 75 79 9 6 6 12 322 1135 Cuts at: 6818 7499 7690 826 Size: 681 191 1471 Fragments arranged by size: 1649 1471 1135 973 681 612 423 322 262 219 191 149 79 75 61 12 9 6 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8335 SmaI CCC'GGG Cuts at: 1872 5662 1872 Size: 3790 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 2883 4003 6439 7340 7602 Size: 197 926 321 619 1120 2436 901 262 Cuts at: 7602 820 Size: 1553 Fragments arranged by size: 2436 1553 1120 926 901 619 321 262 197 SnaBI TAC'GTA Cuts at: 493 6126 493 Size: 5633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8335 SphI G_CATG'C Cuts at: 1132 5693 1132 Size: 4561 3774 SspI AAT'ATT Cuts at: 5 52 4876 6259 5 Size: 47 4824 1383 2081 Fragments arranged by size: 4824 2081 1383 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8335 StyI C'CwwG_G Cuts at: 513 1065 2004 5774 513 Size: 552 939 3770 3074 Fragments arranged by size: 3770 3074 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5479 6362 6379 6532 6717 7162 325 Size: 1255 3899 883 17 153 185 445 1498 Fragments arranged by size: 3899 1498 1255 883 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 4406 6581 Size: 274 80 33 51 1443 222 2207 2175 Cuts at: 6581 8293 96 Size: 1712 138 Fragments arranged by size: 2207 2175 1712 1443 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8295 8327 8295 Size: 32 8303 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8335 XbaI T'CTAG_A Cuts at: 3421 3721 4163 5671 3421 Size: 300 442 1508 6085 Fragments arranged by size: 6085 1508 442 300 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8335 XmaI C'CCGG_G Cuts at: 1870 5660 1870 Size: 3790 4545 XmnI GAAnn'nnTTC Cuts at: 2874 6464 2874 Size: 3590 4745 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6143 276 Size: 53 83 186 936 4609 2468 Fragments arranged by size: 4609 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI