Restriction map for
pFN21AE2546
(Linear) (Six-base) MAPSORT of: KIEE2546.seq Check: 4600 from: 1 to: 3630
KIEE2546.orf
With 182 enzymes: SgfI *
October 24, 2014 10:26 ..
AccI GT'mk_AC
Cuts at: 0 1396 2319 3155 3630
Size: 1396 923 836 475
Fragments arranged by size:
1396 923 836 475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 572 931 1232 1294 2327 2390 3361
Size: 178 394 359 301 62 1033 63 971
Cuts at: 3361 3630
Size: 269
Fragments arranged by size:
1033 971 394 359 301 269 178 63
62
AflII C'TTAA_G
Cuts at: 0 1998 3630
Size: 1998 1632
AflIII A'CryG_T
Cuts at: 0 1091 3033 3630
Size: 1091 1942 597
Fragments arranged by size:
1942 1091 597
AhdI GACnn_n'nnGTC
Cuts at: 0 1392 3630
Size: 1392 2238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1129 3259 3338 3630
Size: 1129 2130 79 292
Fragments arranged by size:
2130 1129 292 79
AlwNI CAG_nnn'CTG
Cuts at: 0 3331 3630
Size: 3331 299
ApaI G_GGCC'C
Cuts at: 0 82 3630
Size: 82 3548
ApoI r'AATT_y
Cuts at: 0 165 280 449 978 1245 2383 2849 3130
Size: 165 115 169 529 267 1138 466 281
Cuts at: 3130 3199 3630
Size: 69 431
Fragments arranged by size:
1138 529 466 431 281 267 169 165
115 69
AseI AT'TA_AT
Cuts at: 0 227 1025 3630
Size: 227 798 2605
Fragments arranged by size:
2605 798 227
AvaI C'yCGr_G
Cuts at: 0 1186 3630
Size: 1186 2444
BanI G'GyrC_C
Cuts at: 0 2862 3630
Size: 2862 768
BanII G_rGCy'C
Cuts at: 0 82 3630
Size: 82 3548
BbsI GAAGACnn'nnnn_
Cuts at: 0 1037 1475 2561 3630
Size: 1037 438 1086 1069
Fragments arranged by size:
1086 1069 1037 438
BbvCI CC'TCA_GC
Cuts at: 0 684 2930 3434 3630
Size: 684 2246 504 196
Fragments arranged by size:
2246 684 504 196
BfrBI ATG'CAT
Cuts at: 0 913 3630
Size: 913 2717
BglII A'GATC_T
Cuts at: 0 153 1297 1443 3630
Size: 153 1144 146 2187
Fragments arranged by size:
2187 1144 153 146
BlpI GC'TnA_GC
Cuts at: 0 362 3062 3630
Size: 362 2700 568
Fragments arranged by size:
2700 568 362
Bme1580I G_kGCm'C
Cuts at: 0 82 2772 3630
Size: 82 2690 858
Fragments arranged by size:
2690 858 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 755 3630
Size: 755 2875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1581 3197 3630
Size: 1581 1616 433
Fragments arranged by size:
1616 1581 433
Bpu10I CC'TnA_GC
Cuts at: 0 684 2930 3434 3630
Size: 684 2246 504 196
Fragments arranged by size:
2246 684 504 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 863 3630
Size: 244 619 2767
Fragments arranged by size:
2767 619 244
BsaI GGTCTCn'nnnn_
Cuts at: 0 1744 2698 3630
Size: 1744 954 932
Fragments arranged by size:
1744 954 932
BsaBI GATnn'nnATC
Cuts at: 0 3511 3630
Size: 3511 119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3195 3225 3630
Size: 3195 30 405
Fragments arranged by size:
3195 405 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 829 1562 3488 3491 3630
Size: 829 733 1926 3 139
Fragments arranged by size:
1926 829 733 139 3
BseYI C'CCAG_C
Cuts at: 0 1888 3408 3630
Size: 1888 1520 222
Fragments arranged by size:
1888 1520 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 873 3630
Size: 334 539 2757
Fragments arranged by size:
2757 539 334
BsiHKAI G_wGCw'C
Cuts at: 0 390 3630
Size: 390 3240
Bsp1286I G_dGCh'C
Cuts at: 0 82 390 2772 3630
Size: 82 308 2382 858
Fragments arranged by size:
2382 858 308 82
BspHI T'CATG_A
Cuts at: 0 2285 3630
Size: 2285 1345
BsrDI GCAATG_nn'
Cuts at: 0 854 3630
Size: 854 2776
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3168 3252 3331 3630
Size: 3168 84 79 299
Fragments arranged by size:
3168 299 84 79
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2972 3630
Size: 2972 658
BstYI r'GATC_y
Cuts at: 0 153 555 1297 1443 3630
Size: 153 402 742 146 2187
Fragments arranged by size:
2187 742 402 153 146
BtsI GCAGTG_nn'
Cuts at: 0 3583 3630
Size: 3583 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1068 1103 3630
Size: 1068 35 2527
Fragments arranged by size:
2527 1068 35
DraI TTT'AAA
Cuts at: 0 2102 2357 3369 3630
Size: 2102 255 1012 261
Fragments arranged by size:
2102 1012 261 255
EaeI y'GGCC_r
Cuts at: 0 1315 3185 3630
Size: 1315 1870 445
Fragments arranged by size:
1870 1315 445
EarI CTCTTCn'nnn_
Cuts at: 0 515 1208 3630
Size: 515 693 2422
Fragments arranged by size:
2422 693 515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 572 931 1232 1294 1581 2327 2390
Size: 178 394 359 301 62 287 746 63
Cuts at: 2390 3197 3361 3630
Size: 807 164 269
Fragments arranged by size:
807 746 394 359 301 287 269 178
164 63 62
EcoO109I rG'GnC_Cy
Cuts at: 0 78 79 2934 3630
Size: 78 1 2855 696
Fragments arranged by size:
2855 696 78 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1452 3630
Size: 1452 2178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 617 649 703 735 1093 1125 1378 1410
Size: 617 32 54 32 358 32 253 32
Cuts at: 1410 1579 1611 2238 2270 3453 3485 3630
Size: 169 32 627 32 1183 32 145
Fragments arranged by size:
1183 627 617 358 253 169 145 54
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1221 3630
Size: 1221 2409
HindIII A'AGCT_T
Cuts at: 0 2214 3630
Size: 2214 1416
HpaI GTT'AAC
Cuts at: 0 1221 3630
Size: 1221 2409
MfeI C'AATT_G
Cuts at: 0 2786 3630
Size: 2786 844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 2155 3630
Size: 366 1789 1475
Fragments arranged by size:
1789 1475 366
MscI TGG'CCA
Cuts at: 0 1317 3187 3630
Size: 1317 1870 443
Fragments arranged by size:
1870 1317 443
MslI CAynn'nnrTG
Cuts at: 0 1111 1477 3038 3630
Size: 1111 366 1561 592
Fragments arranged by size:
1561 1111 592 366
MspA1I CmG'CkG
Cuts at: 0 688 3061 3217 3328 3337 3343 3355 3630
Size: 688 2373 156 111 9 6 12 275
Fragments arranged by size:
2373 688 275 156 111 12 9 6
NdeI CA'TA_TG
Cuts at: 0 234 1820 3630
Size: 234 1586 1810
Fragments arranged by size:
1810 1586 234
NsiI A_TGCA'T
Cuts at: 0 915 3630
Size: 915 2715
NspI r_CATG'y
Cuts at: 0 1095 3037 3183 3630
Size: 1095 1942 146 447
Fragments arranged by size:
1942 1095 447 146
PasI CC'CwG_GG
Cuts at: 0 612 3630
Size: 612 3018
PciI A'CATG_T
Cuts at: 0 1091 3033 3630
Size: 1091 1942 597
Fragments arranged by size:
1942 1091 597
PfoI T'CCnGG_A
Cuts at: 0 307 390 3630
Size: 307 83 3240
Fragments arranged by size:
3240 307 83
PsiI TTA'TAA
Cuts at: 0 2984 3630
Size: 2984 646
PspOMI G'GGCC_C
Cuts at: 0 78 3630
Size: 78 3552
PstI C_TGCA'G
Cuts at: 0 693 1938 3173 3248 3327 3336 3342 3348
Size: 693 1245 1235 75 79 9 6 6
Cuts at: 3348 3360 3630
Size: 12 270
Fragments arranged by size:
1245 1235 693 270 79 75 12 9
6 6
PvuII CAG'CTG
Cuts at: 0 688 3061 3217 3328 3337 3343 3355 3630
Size: 688 2373 156 111 9 6 12 275
Fragments arranged by size:
2373 688 275 156 111 12 9 6
ScaI AGT'ACT
Cuts at: 0 196 2403 3630
Size: 196 2207 1227
Fragments arranged by size:
2207 1227 196
SfcI C'TryA_G
Cuts at: 0 689 1301 1362 1511 1934 2196 3169 3244
Size: 689 612 61 149 423 262 973 75
Cuts at: 3244 3323 3332 3338 3344 3356 3630
Size: 79 9 6 6 12 274
Fragments arranged by size:
973 689 612 423 274 262 149 79
75 61 12 9 6 6
SmlI C'TyrA_G
Cuts at: 0 259 878 1998 3630
Size: 259 619 1120 1632
Fragments arranged by size:
1632 1120 619 259
SspI AAT'ATT
Cuts at: 0 2871 3630
Size: 2871 759
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3474 3630
Size: 3474 156
TatI w'GTAC_w
Cuts at: 0 194 2401 3630
Size: 194 2207 1229
Fragments arranged by size:
2207 1229 194
XbaI T'CTAG_A
Cuts at: 0 1416 1716 2158 3630
Size: 1416 300 442 1472
Fragments arranged by size:
1472 1416 442 300
XmnI GAAnn'nnTTC
Cuts at: 0 869 3630
Size: 869 2761
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AlfI AlwNI ApaI
ApoI AseI AvaI BanI BanII BbsI BbvCI BfrBI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BsrDI BstAPI BstXI BstYI BtsI CspCI DraI EaeI
EarI Eco57MI EcoO109I FalI Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NdeI NsiI NspI
PasI PciI PfoI PsiI PspOMI PstI PvuII ScaI
SfcI SmlI SspI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AloI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BclI BglI BmgBI BmtI BplI BsaAI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII KasI
KpnI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PflMI PmeI PmlI PpiI PpuMI PshAI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI StyI SwaI TstI
Tth111I XcmI XhoI XmaI ZraI