Restriction map for
pFN21AE1795
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 183 ACACGT A^C[GA][CT]GT
BanI 14 GGCACC G^G[CT][GA]CC
BbrPI 184 CACGTG CAC^GTG
BbsI 132 GAAGAC GAAGAC(2/6)
BfaI 493 CTAG C^TAG
BglII 166 AGATCT A^GATCT
BlpI 85 GCTAAGC GC^T[ACGT]AGC
BmyI 187 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 85 GCTAAGC GC^T[ACGT]AGC
BpuAI 132 GAAGAC GAAGAC(2/6)
BsaAI 184 CACGTG [CT]AC^GT[GA]
BsaWI 859 TCCGGT [AT]^CCGG[AT]
BsgI 228 CTGCAC GTGCAG(16/14)
BsmAI 420 GAGAC GTCTC(1/5)
Bsp1286I 187 GTGCCC G[AGT]GC[ACT]^C
BsrDI 875 GCAATG GCAATG(2/0)
BstAPI 274 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 166 AGATCT [GA]^GATC[CT]
CelII 85 GCTAAGC GC^T[ACGT]AGC
DraI 915 TTTAAA TTT^AAA
DraIII 881 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 438 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 657 GAATTC G^AATTC
EcoT14I 746 CCAAGG C^C[AT][AT]GG
FauI 30 CCCGC CCCGC(4/6)
HaeII 793 AGCGCC [GA]GCGC^[CT]
HaeIII 27 GGCC GG^CC
HapII 860 CCGG C^CGG
HpaII 860 CCGG C^CGG
MaeI 493 CTAG C^TAG
MaeII 185 ACGT A^CGT
MflI 166 AGATCT [GA]^GATC[CT]
MspI 860 CCGG C^CGG
PleI 496 GAGTC GAGTC(4/5)
PmaCI 184 CACGTG CAC^GTG
PmlI 184 CACGTG CAC^GTG
PstI 272 CTGCAG CTGCA^G
PvuII 727 CAGCTG CAG^CTG
SfcI 272 CTGCAG C^T[GA][CT]AG
SmlI 651 CTTGAG C^T[CT][GA]AG
SspI 580 AATATT AAT^ATT
StyI 746 CCAAGG C^C[AT][AT]GG
TfiI 105 GATTC G^A[AT]TC
Tsp45I 218 GTGAC ^GT[GC]AC
TspRI 51 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 166 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 19 CGCG CG^CG
43 CGCG
AfaI 242 GTAC GT^AC
638 GTAC
AlwNI 275 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
323 CAGTTCCTG
AvaII 56 GGACC G^G[AT]CC
454 GGACC
BsmFI 55 GGGAC GGGAC(10/14)
3 GTCCC
BsmI 546 GAATGC GAATGC(1/-1)
645 GCATTC
BspMI 226 ACCTGC ACCTGC(4/8)
456 ACCTGC
BsrI 511 ACTGG ACTGG(1/-1)
148 CCAGT
BstUI 19 CGCG CG^CG
43 CGCG
DpnII 167 GATC ^GATC
675 GATC
HinfI 105 GATTC G^A[ACGT]TC
496 GAGTC
MaeIII 208 GTAAC ^GT[ACGT]AC
218 GTGAC
MboI 167 GATC ^GATC
675 GATC
MboII 72 GAAGA GAAGA(8/7)
132 GAAGA
MspA1I 34 CCGCTG C[AC]G^C[GT]G
727 CAGCTG
MvnI 19 CGCG CG^CG
43 CGCG
NdeII 167 GATC ^GATC
675 GATC
PpuMI 55 GGGACCT [GA]G^G[AT]CC[CT]
453 AGGACCT
RsaI 242 GTAC GT^AC
638 GTAC
Sau3AI 167 GATC ^GATC
675 GATC
SfaNI 889 GATGC GCATC(5/9)
523 GCATC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BpmI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
FokI HhaI HinP1I HphI ItaI
MnlI MseI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI Tru9I TseI
Tsp509I