Restriction map for
pFN21AE1795
(Linear) (Six-base) MAPSORT of: KIEE1795.seq Check: 5057 from: 1 to: 939
KIEE1795.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 186 939
Size: 45 141 753
Fragments arranged by size:
753 141 45
AflIII A'CryG_T
Cuts at: 0 183 939
Size: 183 756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 330 939
Size: 330 609
AlwNI CAG_nnn'CTG
Cuts at: 0 280 328 939
Size: 280 48 611
Fragments arranged by size:
611 280 48
ApoI r'AATT_y
Cuts at: 0 559 571 657 939
Size: 559 12 86 282
Fragments arranged by size:
559 282 86 12
BanI G'GyrC_C
Cuts at: 0 14 939
Size: 14 925
BbsI GAAGACnn'nnnn_
Cuts at: 0 139 939
Size: 139 800
BglII A'GATC_T
Cuts at: 0 166 939
Size: 166 773
BlpI GC'TnA_GC
Cuts at: 0 86 939
Size: 86 853
Bme1580I G_kGCm'C
Cuts at: 0 191 939
Size: 191 748
BmrI ACTGGGnnnn_n'
Cuts at: 0 142 939
Size: 142 797
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 677 939
Size: 677 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 438 572 939
Size: 58 380 134 367
Fragments arranged by size:
380 367 134 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 939
Size: 672 267
BsaAI yAC'GTr
Cuts at: 0 186 939
Size: 186 753
BsaWI w'CCGG_w
Cuts at: 0 859 939
Size: 859 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 939
Size: 213 726
BsmI GAATG_Cn'
Cuts at: 0 552 645 939
Size: 552 93 294
Fragments arranged by size:
552 294 93
Bsp1286I G_dGCh'C
Cuts at: 0 191 939
Size: 191 748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 235 465 939
Size: 235 230 474
Fragments arranged by size:
474 235 230
BsrDI GCAATG_nn'
Cuts at: 0 882 939
Size: 882 57
BstAPI GCAn_nnn'nTGC
Cuts at: 0 280 939
Size: 280 659
BstYI r'GATC_y
Cuts at: 0 166 939
Size: 166 773
DraI TTT'AAA
Cuts at: 0 917 939
Size: 917 22
DraIII CAC_nnn'GTG
Cuts at: 0 886 939
Size: 886 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 58 186 438 572 939
Size: 45 13 128 252 134 367
Fragments arranged by size:
367 252 134 128 45 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 442 939
Size: 442 497
EcoO109I rG'GnC_Cy
Cuts at: 0 27 56 454 939
Size: 27 29 398 485
Fragments arranged by size:
485 398 29 27
EcoRI G'AATT_C
Cuts at: 0 657 939
Size: 657 282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 696 939
Size: 696 243
HaeII r_GCGC'y
Cuts at: 0 797 939
Size: 797 142
MspA1I CmG'CkG
Cuts at: 0 36 729 939
Size: 36 693 210
Fragments arranged by size:
693 210 36
PmlI CAC'GTG
Cuts at: 0 186 939
Size: 186 753
PpuMI rG'GwC_Cy
Cuts at: 0 56 454 939
Size: 56 398 485
Fragments arranged by size:
485 398 56
PstI C_TGCA'G
Cuts at: 0 276 939
Size: 276 663
PvuII CAG'CTG
Cuts at: 0 729 939
Size: 729 210
SfcI C'TryA_G
Cuts at: 0 272 939
Size: 272 667
SmlI C'TyrA_G
Cuts at: 0 651 939
Size: 651 288
SspI AAT'ATT
Cuts at: 0 582 939
Size: 582 357
StyI C'CwwG_G
Cuts at: 0 746 939
Size: 746 193
TatI w'GTAC_w
Cuts at: 0 637 939
Size: 637 302
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI BanI BbsI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaWI
BsgI BsmI Bsp1286I BspMI BsrDI BstAPI BstYI DraI
DraIII Eco57MI EcoNI EcoO109I EcoRI FalI HaeII MspA1I
PmlI PpuMI PstI PvuII SfcI SmlI SspI StyI
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BmgBI BmtI Bpu10I BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DrdI EaeI EagI EarI EciI EcoICRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI