Restriction map for
pFN21AB9706
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI HphI KpnI MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MunI NcoI NdeI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SbfI ScaI SexAI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 863 ACGTGT A^C[GA][CT]GT
AluI 383 AGCT AG^CT
AlwI 831 GATCC GGATC(4/5)
AlwNI 666 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 806 AGCGCT AGC^GCT
AspHI 415 GTGCTC G[AT]GC[AT]^C
BfaI 86 CTAG C^TAG
BpmI 202 CTGGAG CTGGAG(16/14)
BsaI 977 GAGACC GGTCTC(1/5)
BseRI 361 GAGGAG GAGGAG(10/8)
BsgI 672 CTGCAC GTGCAG(16/14)
BsiHKAI 415 GTGCTC G[AT]GC[AT]^C
BsmAI 977 GAGAC GTCTC(1/5)
Bsp1407I 866 TGTACA T^GTACA
BspMI 44 ACCTGC ACCTGC(4/8)
BsrDI 509 GCAATG GCAATG(2/0)
BsrGI 866 TGTACA T^GTACA
BssHII 207 GCGCGC G^CGCGC
BstAPI 665 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 830 AGATCC [GA]^GATC[CT]
DdeI 431 CTGAG C^T[ACGT]AG
DpnII 831 GATC ^GATC
DrdI 919 GACCGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 806 AGCGCT AGC^GCT
HinfI 945 GAATC G^A[ACGT]TC
MaeI 86 CTAG C^TAG
MboI 831 GATC ^GATC
MflI 830 AGATCC [GA]^GATC[CT]
MseI 860 TTAA T^TAA
NaeI 404 GCCGGC GCC^GGC
NdeII 831 GATC ^GATC
NgoMIV 404 GCCGGC G^CCGGC
NheI 85 GCTAGC G^CTAGC
NotI 346 GCGGCCGC GC^GGCCGC
NspI 38 GCATGT [GA]CATG^[CT]
PstI 985 CTGCAG CTGCA^G
Sau3AI 831 GATC ^GATC
SfiI 348 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 866 TGTACA T^GTACA
TaqI 533 TCGA T^CGA
TfiI 945 GAATC G^A[AT]TC
Tru9I 860 TTAA T^TAA
TthHB8I 533 TCGA T^CGA
XhoII 830 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 426 GGCGCC G[GA]^CG[CT]C
475 GGCGCC
ApaI 559 GGGCCC GGGCC^C
738 GGGCCC
AvaII 641 GGACC G^G[AT]CC
794 GGTCC
BanI 426 GGCGCC G^G[CT][GA]CC
475 GGCGCC
BbeI 426 GGCGCC GGCGC^C
475 GGCGCC
BsaHI 426 GGCGCC G[GA]^CG[CT]C
475 GGCGCC
BstF5I 902 GGATG GGATG(2/0)
953 GGATG
EcoT14I 677 CCAAGG C^C[AT][AT]GG
701 CCAAGG
FokI 902 GGATG GGATG(9/13)
953 GGATG
Hin1I 426 GGCGCC G[GA]^CG[CT]C
475 GGCGCC
KasI 426 GGCGCC G^GCGCC
475 GGCGCC
NarI 426 GGCGCC GG^CGCC
475 GGCGCC
NlaIII 39 CATG CATG^
372 CATG
SapI 765 GAAGAGC GCTCTTC(1/4)
417 GCTCTTC
SfaNI 903 GATGC GCATC(5/9)
515 GCATC
SfcI 492 CTACAG C^T[GA][CT]AG
985 CTGCAG
SfoI 426 GGCGCC GGC^GCC
475 GGCGCC
SmaI 556 CCCGGG CCC^GGG
744 CCCGGG
StyI 677 CCAAGG C^C[AT][AT]GG
701 CCAAGG
TspRI 915 CACTG CA[GC]TG[ACGT][ACGT]^
996 CACTG
XmaCI 556 CCCGGG C^CCGGG
744 CCCGGG
XmaI 556 CCCGGG C^CCGGG
744 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AvaI BanII
BbvI BcnI BglI BmyI BsaJI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI Ksp632I KspI
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
RsaI SacII Sau96I ScrFI TseI