Restriction map for
pFN21AB9706
(Linear) (Six-base) MAPSORT of: KIBB9706.seq Check: 8130 from: 1 to: 1014
KIBB9706.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AfeI AGC'GCT
Cuts at: 0 808 1014
Size: 808 206
AflIII A'CryG_T
Cuts at: 0 863 1014
Size: 863 151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 1014
Size: 61 953
AlwNI CAG_nnn'CTG
Cuts at: 0 671 1014
Size: 671 343
ApaI G_GGCC'C
Cuts at: 0 563 742 1014
Size: 563 179 272
Fragments arranged by size:
563 272 179
AvaI C'yCGr_G
Cuts at: 0 556 610 744 1014
Size: 556 54 134 270
Fragments arranged by size:
556 270 134 54
BanI G'GyrC_C
Cuts at: 0 426 475 1014
Size: 426 49 539
Fragments arranged by size:
539 426 49
BanII G_rGCy'C
Cuts at: 0 563 701 742 1014
Size: 563 138 41 272
Fragments arranged by size:
563 272 138 41
BbeI G_GCGC'C
Cuts at: 0 430 479 1014
Size: 430 49 535
Fragments arranged by size:
535 430 49
BglI GCCn_nnn'nGGC
Cuts at: 0 355 364 473 512 1014
Size: 355 9 109 39 502
Fragments arranged by size:
502 355 109 39 9
Bme1580I G_kGCm'C
Cuts at: 0 563 742 1014
Size: 563 179 272
Fragments arranged by size:
563 272 179
BmgBI CAC'GTC
Cuts at: 0 162 414 1014
Size: 162 252 600
Fragments arranged by size:
600 252 162
BmtI G_CTAG'C
Cuts at: 0 89 1014
Size: 89 925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1014
Size: 223 791
Bpu10I CC'TnA_GC
Cuts at: 0 431 1014
Size: 431 583
BsaI GGTCTCn'nnnn_
Cuts at: 0 971 1014
Size: 971 43
BsaHI Gr'CG_yC
Cuts at: 0 427 476 1014
Size: 427 49 538
Fragments arranged by size:
538 427 49
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 376 1014
Size: 376 638
BseYI C'CCAG_C
Cuts at: 0 788 1014
Size: 788 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 657 1014
Size: 657 357
BsiEI CG_ry'CG
Cuts at: 0 350 359 409 1014
Size: 350 9 50 605
Fragments arranged by size:
605 350 50 9
BsiHKAI G_wGCw'C
Cuts at: 0 419 1014
Size: 419 595
Bsp1286I G_dGCh'C
Cuts at: 0 419 563 701 742 1014
Size: 419 144 138 41 272
Fragments arranged by size:
419 272 144 138 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 1014
Size: 53 961
BsrDI GCAATG_nn'
Cuts at: 0 516 1014
Size: 516 498
BsrFI r'CCGG_y
Cuts at: 0 404 435 920 1014
Size: 404 31 485 94
Fragments arranged by size:
485 404 94 31
BsrGI T'GTAC_A
Cuts at: 0 866 1014
Size: 866 148
BssHII G'CGCG_C
Cuts at: 0 207 1014
Size: 207 807
BstAPI GCAn_nnn'nTGC
Cuts at: 0 671 1014
Size: 671 343
BstYI r'GATC_y
Cuts at: 0 830 1014
Size: 830 184
BtgI C'CryG_G
Cuts at: 0 225 350 395 470 1014
Size: 225 125 45 75 544
Fragments arranged by size:
544 225 125 75 45
DrdI GACnn_nn'nnGTC
Cuts at: 0 925 1014
Size: 925 89
EaeI y'GGCC_r
Cuts at: 0 21 217 347 356 368 406 1014
Size: 21 196 130 9 12 38 608
Fragments arranged by size:
608 196 130 38 21 12 9
EagI C'GGCC_G
Cuts at: 0 347 356 406 1014
Size: 347 9 50 608
Fragments arranged by size:
608 347 50 9
EarI CTCTTCn'nnn_
Cuts at: 0 147 424 760 1014
Size: 147 277 336 254
Fragments arranged by size:
336 277 254 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1014
Size: 223 791
EcoO109I rG'GnC_Cy
Cuts at: 0 560 738 739 1014
Size: 560 178 1 275
Fragments arranged by size:
560 275 178 1
HaeII r_GCGC'y
Cuts at: 0 430 479 556 810 1014
Size: 430 49 77 254 204
Fragments arranged by size:
430 254 204 77 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 878 910 1014
Size: 878 32 104
Fragments arranged by size:
878 104 32
KasI G'GCGC_C
Cuts at: 0 426 475 1014
Size: 426 49 539
Fragments arranged by size:
539 426 49
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 509 1014
Size: 170 339 505
Fragments arranged by size:
505 339 170
MspA1I CmG'CkG
Cuts at: 0 227 266 352 397 472 722 792 1014
Size: 227 39 86 45 75 250 70 222
Fragments arranged by size:
250 227 222 86 75 70 45 39
NaeI GCC'GGC
Cuts at: 0 406 1014
Size: 406 608
NarI GG'CG_CC
Cuts at: 0 427 476 1014
Size: 427 49 538
Fragments arranged by size:
538 427 49
NgoMIV G'CCGG_C
Cuts at: 0 404 1014
Size: 404 610
NheI G'CTAG_C
Cuts at: 0 85 1014
Size: 85 929
NotI GC'GGCC_GC
Cuts at: 0 347 1014
Size: 347 667
NspI r_CATG'y
Cuts at: 0 42 1014
Size: 42 972
PfoI T'CCnGG_A
Cuts at: 0 796 1014
Size: 796 218
PspOMI G'GGCC_C
Cuts at: 0 559 738 1014
Size: 559 179 276
Fragments arranged by size:
559 276 179
PstI C_TGCA'G
Cuts at: 0 989 1014
Size: 989 25
SacII CC_GC'GG
Cuts at: 0 228 353 398 473 1014
Size: 228 125 45 75 541
Fragments arranged by size:
541 228 125 75 45
SapI GCTCTTCn'nnn_
Cuts at: 0 424 760 1014
Size: 424 336 254
Fragments arranged by size:
424 336 254
SfcI C'TryA_G
Cuts at: 0 492 985 1014
Size: 492 493 29
Fragments arranged by size:
493 492 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 355 1014
Size: 355 659
SfoI GGC'GCC
Cuts at: 0 428 477 1014
Size: 428 49 537
Fragments arranged by size:
537 428 49
SmaI CCC'GGG
Cuts at: 0 558 746 1014
Size: 558 188 268
Fragments arranged by size:
558 268 188
SrfI GCCC'GGGC
Cuts at: 0 558 1014
Size: 558 456
StyI C'CwwG_G
Cuts at: 0 677 701 1014
Size: 677 24 313
Fragments arranged by size:
677 313 24
TatI w'GTAC_w
Cuts at: 0 866 1014
Size: 866 148
XmaI C'CCGG_G
Cuts at: 0 556 744 1014
Size: 556 188 270
Fragments arranged by size:
556 270 188
Enzymes that do cut:
AfeI AflIII AlfI AlwNI ApaI AvaI BanI BanII
BbeI BglI Bme1580I BmgBI BmtI BpmI Bpu10I BsaI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BsrGI BssHII BstAPI BstYI BtgI DrdI
EaeI EagI EarI Eco57MI EcoO109I HaeII Hin4I KasI
MmeI MspA1I NaeI NarI NgoMIV NheI NotI NspI
PfoI PspOMI PstI SacII SapI SfcI SfiI SfoI
SmaI SrfI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BplI BpuEI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NdeI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SbfI ScaI SexAI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmnI ZraI