################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MboII MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 220 AAATTT [GA]^AATT[CT] ApaI 140 GGGCCC GGGCC^C ApoI 220 AAATTT [GA]^AATT[CT] Asp700I 314 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 49 GGATCC G^GATCC BfaI 104 CTAG C^TAG BpmI 113 CTGGAG CTGGAG(16/14) BsaI 382 GGTCTC GGTCTC(1/5) BsaJI 354 CCTGGG C^C[ACGT][ACGT]GG BspMI 232 GCAGGT ACCTGC(4/8) BssKI 354 CCTGG ^CC[ACGT]GG BstAPI 232 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 354 CCTGG CC^[AT]GG BstYI 49 GGATCC [GA]^GATC[CT] CfoI 11 GCGC GCG^C DdeI 158 CTCAG C^T[ACGT]AG DraII 139 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoO109I 139 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRII 354 CCTGG ^CC[AT]GG HaeIII 141 GGCC GG^CC HhaI 11 GCGC GCG^C HinP1I 11 GCGC G^CGC MaeI 104 CTAG C^TAG MflI 49 GGATCC [GA]^GATC[CT] MseI 134 TTAA T^TAA MspA1I 334 CAGCTG C[AC]G^C[GT]G MvaI 354 CCTGG CC^[AT]GG NdeI 344 CATATG CA^TATG NlaIII 423 CATG CATG^ PvuII 334 CAGCTG CAG^CTG ScrFI 354 CCTGG CC^[ACGT]GG SfaNI 79 GCATC GCATC(5/9) TaqI 93 TCGA T^CGA Tru9I 134 TTAA T^TAA TthHB8I 93 TCGA T^CGA XhoII 49 GGATCC [GA]^GATC[CT] XmnI 314 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwNI 233 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 331 CAGCAGCTG BanII 120 GGGCTC G[GA]GC[CT]^C 140 GGGCCC BmyI 120 GGGCTC G[AGT]GC[ACT]^C 140 GGGCCC BsgI 24 CTGCAC GTGCAG(16/14) 230 GTGCAG Bsp1286I 120 GGGCTC G[AGT]GC[ACT]^C 140 GGGCCC BsrI 112 ACTGG ACTGG(1/-1) 185 CCAGT Cfr13I 140 GGGCC G^G[ACGT]CC 141 GGCCC DpnII 39 GATC ^GATC 50 GATC MboI 39 GATC ^GATC 50 GATC NdeII 39 GATC ^GATC 50 GATC Sau3AI 39 GATC ^GATC 50 GATC Sau96I 140 GGGCC G^G[ACGT]CC 141 GGCCC Tsp509I 72 AATT ^AATT 221 AATT TspRI 83 CAGTG CA[GC]TG[ACGT][ACGT]^ 186 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI AlwI BbvI BsmAI Cac8I Fnu4HI ItaI MnlI MwoI NlaIV TseI