################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FseI FspI HindIII HpaI KpnI KspI MamI MluI MluNI MroI MscI NciI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 497 GTCGAC GT^[AC][GT]AC AccII 972 CGCG CG^CG AlwNI 976 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG BamHI 868 GGATCC G^GATCC BanI 6 GGCGCC G^G[CT][GA]CC BanII 671 GGGCTC G[GA]GC[CT]^C BbeI 6 GGCGCC GGCGC^C BclI 956 TGATCA T^GATCA BpmI 889 CTGGAG CTGGAG(16/14) BsaI 909 GGTCTC GGTCTC(1/5) BsaWI 186 TCCGGT [AT]^CCGG[AT] BsmFI 750 GGGAC GGGAC(10/14) BsmI 838 GCATTC GAATGC(1/-1) BspMI 23 ACCTGC ACCTGC(4/8) BsrDI 590 GCAATG GCAATG(2/0) BsrFI 162 GCCGGC [GA]^CCGG[CT] BssHII 971 GCGCGC G^CGCGC BstUI 972 CGCG CG^CG BstXI 784 CCAGAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 162 GCCGGC [GA]^CCGG[CT] Cfr13I 309 GGCCC G^G[ACGT]CC DdeI 194 CTAAG C^T[ACGT]AG EcoT22I 896 ATGCAT ATGCA^T FauI 311 CCCGC CCCGC(4/6) FbaI 956 TGATCA T^GATCA HaeII 6 GGCGCC [GA]GCGC^[CT] HgaI 17 GCGTC GACGC(5/10) HinfI 279 GATTC G^A[ACGT]TC KasI 6 GGCGCC G^GCGCC MfeI 650 CAATTG C^AATTG MslI 715 CATCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 304 CAGCTG C[AC]G^C[GT]G MunI 650 CAATTG C^AATTG MvnI 972 CGCG CG^CG NaeI 162 GCCGGC GCC^GGC NarI 6 GGCGCC GG^CGCC NgoMIV 162 GCCGGC G^CCGGC NsiI 896 ATGCAT ATGCA^T PshAI 903 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 167 CTGCAG CTGCA^G PvuII 304 CAGCTG CAG^CTG SalI 497 GTCGAC G^TCGAC Sau96I 309 GGCCC G^G[ACGT]CC SfoI 6 GGCGCC GGC^GCC TfiI 279 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 6 GGCGCC G[GA]^CG[CT]C 16 GGCGTC AfaI 235 GTAC GT^AC 455 GTAC AspHI 472 GTGCTC G[AT]GC[AT]^C 943 GAGCAC BbvI 655 GCAGC GCAGC(8/12) 166 GCTGC BfaI 424 CTAG C^TAG 916 CTAG BsaHI 6 GGCGCC G[GA]^CG[CT]C 16 GGCGTC BsaJI 558 CCATGG C^C[ACGT][ACGT]GG 821 CCATGG BseRI 322 CTCCTC GAGGAG(10/8) 826 GAGGAG BsiHKAI 472 GTGCTC G[AT]GC[AT]^C 943 GAGCAC BsiYI 238 CCAGACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 321 CCTCCTCTTGG BslI 238 CCAGACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 321 CCTCCTCTTGG BsrI 242 ACTGG ACTGG(1/-1) 11 CCAGT BssKI 629 CCAGG ^CC[ACGT]GG 888 CCTGG BstNI 629 CCAGG CC^[AT]GG 888 CCTGG DraI 342 TTTAAA TTT^AAA 880 TTTAAA DsaI 558 CCATGG C^C[GA][CT]GG 821 CCATGG EcoRII 629 CCAGG ^CC[AT]GG 888 CCTGG EcoT14I 558 CCATGG C^C[AT][AT]GG 821 CCATGG HaeIII 309 GGCC GG^CC 1002 GGCC HapII 163 CCGG C^CGG 187 CCGG Hin1I 6 GGCGCC G[GA]^CG[CT]C 16 GGCGTC HpaII 163 CCGG C^CGG 187 CCGG HphI 61 TCACC GGTGA(8/7) 418 TCACC MaeI 424 CTAG C^TAG 916 CTAG MaeIII 220 GTGAC ^GT[ACGT]AC 535 GTCAC MspI 163 CCGG C^CGG 187 CCGG MvaI 629 CCAGG CC^[AT]GG 888 CCTGG NcoI 558 CCATGG C^CATGG 821 CCATGG RsaI 235 GTAC GT^AC 455 GTAC ScrFI 629 CCAGG CC^[ACGT]GG 888 CCTGG SfcI 167 CTGCAG C^T[GA][CT]AG 845 CTATAG StyI 558 CCATGG C^C[AT][AT]GG 821 CCATGG TseI 166 GCTGC G^C[AT]GC 655 GCAGC Tsp45I 220 GTGAC ^GT[GC]AC 535 GTCAC TspRI 12 CAGTG CA[GC]TG[ACGT][ACGT]^ 810 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BmyI BsmAI Bsp1286I BstF5I BstYI Cac8I CfoI DpnII EarI Fnu4HI FokI HhaI HinP1I HincII HindII ItaI Ksp632I MaeII MboI MboII MflI MnlI MseI MwoI NdeII NlaIII NlaIV Sau3AI SfaNI TaqI Tru9I Tsp509I TthHB8I XhoII