(Circular) (Six-base) MAPSORT of: pFN21AE5429.seq Check: 3145 from: 1 to: 5710 pFN21AE5429 5710 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2037 2037 Size: 5710 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2503 3053 1937 Size: 566 550 4594 Fragments arranged by size: 4594 566 550 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2166 2388 2495 3773 5157 5425 1632 Size: 534 222 107 1278 1384 268 1917 Fragments arranged by size: 1917 1384 1278 534 268 222 107 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 2986 3453 5025 2986 Size: 467 1572 3671 Fragments arranged by size: 3671 1572 467 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 2055 2119 2793 2882 3019 3245 3397 5506 Size: 562 64 674 89 137 226 152 2109 Cuts at: 5506 1493 Size: 1697 Fragments arranged by size: 2109 1697 674 562 226 152 137 89 64 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 3035 1870 Size: 73 1092 4545 Fragments arranged by size: 4545 1092 73 BamHI G'GATC_C Cuts at: 2873 3040 3407 2873 Size: 167 367 5176 Fragments arranged by size: 5176 367 167 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 3031 4486 618 Size: 325 217 627 224 1020 1455 1842 Fragments arranged by size: 1842 1455 1020 627 325 224 217 BanII G_rGCy'C Cuts at: 1391 2680 3029 1391 Size: 1289 349 4072 Fragments arranged by size: 4072 1289 349 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 5710 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 2961 1548 Size: 1413 4297 BfrBI ATG'CAT Cuts at: 2903 2903 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4321 136 Size: 107 122 71 631 605 337 2312 1525 Fragments arranged by size: 2312 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 3771 4927 1790 Size: 1981 1156 2573 Fragments arranged by size: 2573 1981 1156 BmgBI CAC'GTC Cuts at: 1318 2323 2967 1318 Size: 1005 644 4061 Fragments arranged by size: 4061 1005 644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2010 2257 3441 Size: 416 53 83 399 24 593 247 1184 Cuts at: 3441 4399 442 Size: 958 1753 Fragments arranged by size: 1753 1184 958 593 416 399 247 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2915 4370 1239 Size: 1676 1455 2579 Fragments arranged by size: 2579 1676 1455 Bpu10I CC'TnA_GC Cuts at: 2199 2199 Size: 5710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2920 4373 882 Size: 617 1421 1453 2219 Fragments arranged by size: 2219 1453 1421 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2022 3517 3899 Size: 53 83 186 936 479 10 1495 382 Cuts at: 3899 4534 275 Size: 635 1451 Fragments arranged by size: 1495 1451 936 635 479 382 186 83 53 10 BsaWI w'CCGG_w Cuts at: 2191 4143 4541 4815 4962 5373 2191 Size: 1952 398 274 147 411 2528 Fragments arranged by size: 2528 1952 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 2846 1186 Size: 1132 528 4050 Fragments arranged by size: 4050 1132 528 BseYI C'CCAG_C Cuts at: 2336 4913 5409 2336 Size: 2577 496 2637 Fragments arranged by size: 2637 2577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2481 2952 3771 3856 4927 2481 Size: 471 819 85 1071 3264 Fragments arranged by size: 3264 1071 819 471 85 BsmI GAATG_Cn' Cuts at: 2843 3221 3314 2843 Size: 378 93 5239 Fragments arranged by size: 5239 378 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2481 2680 2952 3029 3771 3856 4927 Size: 399 691 199 272 77 742 85 1071 Cuts at: 4927 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 691 399 272 199 85 77 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2037 3065 844 Size: 1193 1028 3489 Fragments arranged by size: 3489 1193 1028 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2602 4205 4379 65 Size: 1183 1354 1603 174 1396 Fragments arranged by size: 1603 1396 1354 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2167 4354 4541 1700 Size: 110 357 2187 187 2869 Fragments arranged by size: 2869 2187 357 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 2976 1925 Size: 1051 4659 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstXI CCAn_nnnn'nTGG Cuts at: 2796 2796 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2768 2873 2982 3040 3407 3792 3809 5261 Size: 796 105 109 58 367 385 17 1452 Cuts at: 5261 5347 5359 5705 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 796 385 367 346 109 105 86 58 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2563 2826 513 Size: 552 939 559 263 3397 Fragments arranged by size: 3397 939 559 552 263 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 2349 2887 3014 2349 Size: 538 127 5045 Fragments arranged by size: 5045 538 127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675 Size: 54 1425 50 275 18 1327 889 1629 Cuts at: 5675 8 Size: 43 Fragments arranged by size: 1629 1425 1327 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2068 2336 2364 2514 3640 1353 Size: 715 268 28 150 1126 3423 Fragments arranged by size: 3423 1126 715 268 150 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2388 2495 2915 3773 4370 5157 Size: 393 534 222 107 420 858 597 787 Cuts at: 5157 5425 1239 Size: 268 1524 Fragments arranged by size: 1524 858 787 597 534 420 393 268 222 107 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2015 4857 1678 Size: 337 2842 2531 Fragments arranged by size: 2842 2531 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2365 2397 2511 2543 4351 Size: 32 135 32 793 32 114 32 1808 Cuts at: 4351 4383 4425 4457 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1808 793 135 114 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2108 2356 2504 3054 3300 669 Size: 988 281 170 248 148 550 246 3079 Fragments arranged by size: 3079 988 550 281 248 246 170 148 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KasI G'GCGC_C Cuts at: 2011 2011 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 2655 3309 2655 Size: 654 5056 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2724 3668 4027 4186 518 Size: 648 1558 944 359 159 2042 Fragments arranged by size: 2042 1558 944 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2311 3126 3803 4951 5196 1606 Size: 705 815 677 1148 245 2120 Fragments arranged by size: 2120 1148 815 705 677 245 NaeI GCC'GGC Cuts at: 2169 2169 Size: 5710 NarI GG'CG_CC Cuts at: 2012 2012 Size: 5710 NcoI C'CATG_G Cuts at: 513 1065 2004 2563 2826 513 Size: 552 939 559 263 3397 Fragments arranged by size: 3397 939 559 552 263 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NgoMIV G'CCGG_C Cuts at: 2167 2167 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NsiI A_TGCA'T Cuts at: 2905 2905 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 3068 4613 1132 Size: 97 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PfoI T'CCnGG_A Cuts at: 2892 2892 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2912 1613 Size: 1299 4411 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 2176 3062 830 Size: 1346 886 3478 Fragments arranged by size: 3478 1346 886 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2311 2311 Size: 5710 SalI G'TCGA_C Cuts at: 1936 2502 3052 1936 Size: 566 550 4594 Fragments arranged by size: 4594 566 550 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2172 2850 3058 4193 4874 5065 826 Size: 219 1127 678 208 1135 681 191 1471 Fragments arranged by size: 1471 1135 1127 681 678 219 208 191 SfoI GGC'GCC Cuts at: 2013 2013 Size: 5710 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3814 4715 4977 820 Size: 197 926 1871 901 262 1553 Fragments arranged by size: 1871 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2563 2826 3149 513 Size: 552 939 559 263 323 3074 Fragments arranged by size: 3074 939 559 552 323 263 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3737 3754 3907 4092 4537 325 Size: 1255 2157 17 153 185 445 1498 Fragments arranged by size: 2157 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI