(Circular) (Six-base) MAPSORT of: pFN21AE2156.seq Check: 8146 from: 1 to: 5962 pFN21AE2156 5962 bp With 182 enzymes: SgfI * July 11, 2011 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2168 3305 1937 Size: 231 1137 4594 Fragments arranged by size: 4594 1137 231 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2148 2522 4025 5409 5677 1632 Size: 516 374 1503 1384 268 1917 Fragments arranged by size: 1917 1503 1384 516 374 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 2504 2713 4861 5838 1203 Size: 1301 209 2148 977 1327 Fragments arranged by size: 2148 1327 1301 977 209 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2143 2175 2143 Size: 32 5930 AlwNI CAG_nnn'CTG Cuts at: 2728 3705 5277 2728 Size: 977 1572 3413 Fragments arranged by size: 3413 1572 977 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2030 2801 3003 3271 3497 3649 5758 1493 Size: 537 771 202 268 226 152 2109 1697 Fragments arranged by size: 2109 1697 771 537 268 226 202 152 AseI AT'TA_AT Cuts at: 160 1032 2918 4516 160 Size: 872 1886 1598 1606 Fragments arranged by size: 1886 1606 1598 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2907 3287 1870 Size: 73 964 380 4545 Fragments arranged by size: 4545 964 380 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3283 4738 618 Size: 325 217 627 1496 1455 1842 Fragments arranged by size: 1842 1496 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3281 1391 Size: 1890 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3082 928 Size: 971 1183 3808 Fragments arranged by size: 3808 1183 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BfrBI ATG'CAT Cuts at: 2279 2279 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2131 3109 5957 2131 Size: 978 2848 2136 Fragments arranged by size: 2848 2136 978 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 4023 5179 1790 Size: 2233 1156 2573 Fragments arranged by size: 2573 2233 1156 BmgBI CAC'GTC Cuts at: 1318 2584 1318 Size: 1266 4696 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2574 1056 Size: 1518 4444 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2663 2663 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4622 1239 Size: 3383 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3240 4087 4952 5250 3240 Size: 847 865 298 3952 Fragments arranged by size: 3952 865 847 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4625 882 Size: 617 3126 2219 Fragments arranged by size: 3126 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2714 3753 493 Size: 711 1510 1039 2702 Fragments arranged by size: 2702 1510 1039 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5737 Fragments arranged by size: 5737 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2478 1186 Size: 1292 4670 BseYI C'CCAG_C Cuts at: 5165 5661 5165 Size: 496 5466 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2073 1163 Size: 573 337 5052 Fragments arranged by size: 5052 573 337 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2658 3412 4173 5201 1540 Size: 275 186 657 754 761 1028 2301 Fragments arranged by size: 2301 1028 761 754 657 275 186 BsiHKAI G_wGCw'C Cuts at: 2767 4023 4108 5179 2767 Size: 1256 85 1071 3550 Fragments arranged by size: 3550 1256 1071 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2767 3281 4023 4108 5179 1391 Size: 399 977 514 742 85 1071 2174 Fragments arranged by size: 2174 1071 977 742 514 399 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2527 3851 5581 2527 Size: 1324 1730 2908 Fragments arranged by size: 2908 1730 1324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3317 844 Size: 2473 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3231 4606 4793 1700 Size: 110 1421 1375 187 2869 Fragments arranged by size: 2869 1421 1375 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2131 2223 2833 3109 3292 3659 4044 4061 Size: 159 92 610 276 183 367 385 17 Cuts at: 4061 5513 5599 5611 5957 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 610 385 367 346 276 183 159 92 86 17 12 BstZ17I GTA'TAC Cuts at: 2169 2169 Size: 5962 BtgI C'CryG_G Cuts at: 513 1065 2004 2758 513 Size: 552 939 754 3717 Fragments arranged by size: 3717 939 754 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2061 3478 4272 4292 2061 Size: 1417 794 20 3731 Fragments arranged by size: 3731 1417 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2708 3409 4298 Size: 54 1425 50 275 18 878 701 889 Cuts at: 4298 5927 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 878 701 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3195 3892 1353 Size: 1842 697 3423 Fragments arranged by size: 3423 1842 697 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2522 4025 4622 5409 5677 1239 Size: 393 516 374 1503 597 787 268 1524 Fragments arranged by size: 1524 1503 787 597 516 393 374 268 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2722 2754 3115 3147 4603 Size: 32 135 32 1150 32 361 32 1456 Cuts at: 4603 4635 4677 4709 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1456 1150 361 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2256 3306 3552 669 Size: 988 281 318 1050 246 3079 Fragments arranged by size: 3079 1050 988 318 281 246 HindIII A'AGCT_T Cuts at: 748 2879 748 Size: 2131 3831 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 2100 3561 2100 Size: 1461 4501 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2480 5076 5260 1305 Size: 1175 2596 184 2007 Fragments arranged by size: 2596 2007 1175 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3920 4279 4438 518 Size: 648 2754 359 159 2042 Fragments arranged by size: 2754 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2828 3196 3378 4055 5203 5448 1606 Size: 1222 368 182 677 1148 245 2120 Fragments arranged by size: 2120 1222 1148 677 368 245 182 NcoI C'CATG_G Cuts at: 513 1065 2004 2758 513 Size: 552 939 754 3717 Fragments arranged by size: 3717 939 754 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 2570 1052 Size: 1518 4444 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NsiI A_TGCA'T Cuts at: 2281 2281 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2508 3184 3320 4865 1132 Size: 97 1279 676 136 1545 2229 Fragments arranged by size: 2229 1545 1279 676 136 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 2504 4861 2504 Size: 2357 3605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PmlI CAC'GTG Cuts at: 2714 2714 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 3314 830 Size: 2484 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 3196 3196 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2808 3055 3310 4445 5126 5317 826 Size: 219 1763 247 255 1135 681 191 1471 Fragments arranged by size: 1763 1471 1135 681 255 247 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 2909 3289 1872 Size: 1037 380 4545 Fragments arranged by size: 4545 1037 380 SmlI C'TyrA_G Cuts at: 820 1017 1943 3255 4066 4967 5229 820 Size: 197 926 1312 811 901 262 1553 Fragments arranged by size: 1553 1312 926 901 811 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 3217 152 Size: 3065 2897 SphI G_CATG'C Cuts at: 1132 3184 3320 1132 Size: 2052 136 3774 Fragments arranged by size: 3774 2052 136 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2004 2758 3401 513 Size: 552 939 754 643 3074 Fragments arranged by size: 3074 939 754 643 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2076 4208 5920 Size: 274 80 33 51 1443 99 2132 1712 Cuts at: 5920 96 Size: 138 Fragments arranged by size: 2132 1712 1443 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2788 2820 5922 5954 2788 Size: 32 3102 32 2796 Fragments arranged by size: 3102 2796 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2180 2180 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 2907 3287 1870 Size: 1037 380 4545 Fragments arranged by size: 4545 1037 380 XmnI GAAnn'nnTTC Cuts at: 2378 2535 2781 3006 4091 2378 Size: 157 246 225 1085 4249 Fragments arranged by size: 4249 1085 246 225 157 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI