Restriction map for
pFN21AE6636
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 202 GTCTAC GT^[AC][GT]AC
AcsI 690 AAATTC [GA]^AATT[CT]
AflIII 474 ACATGT A^C[GA][CT]GT
AlwNI 272 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 679 CCTAAGG CC^T[ACGT]AGG
ApaI 635 GGGCCC GGGCC^C
ApoI 690 AAATTC [GA]^AATT[CT]
AspHI 154 GTGCTC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BanI 146 GGCACC G^G[CT][GA]CC
BbsI 175 GTCTTC GAAGAC(2/6)
BfaI 133 CTAG C^TAG
BglII 847 AGATCT A^GATCT
BpuAI 175 GTCTTC GAAGAC(2/6)
BsgI 355 CTGCAC GTGCAG(16/14)
BsiHKAI 154 GTGCTC G[AT]GC[AT]^C
Bsp1407I 347 TGTACA T^GTACA
BspLU11I 474 ACATGT A^CATGT
BsrDI 666 GCAATG GCAATG(2/0)
BsrFI 166 ACCGGC [GA]^CCGG[CT]
BsrGI 347 TGTACA T^GTACA
BstEII 162 GGTCACC G^GT[ACGT]ACC
BstPI 162 GGTCACC G^GT[ACGT]ACC
Bsu36I 679 CCTAAGG CC^T[ACGT]AGG
Cfr10I 166 ACCGGC [GA]^CCGG[CT]
DraIII 148 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 679 CCTAAGG CC^T[ACGT]AGG
EcoO65I 162 GGTCACC G^GT[ACGT]ACC
FauI 582 CCCGC CCCGC(4/6)
HapII 167 CCGG C^CGG
HincII 328 GTTAAC GT[CT]^[GA]AC
HindII 328 GTTAAC GT[CT]^[GA]AC
HinfI 781 GATTC G^A[ACGT]TC
HpaI 328 GTTAAC GTT^AAC
HpaII 167 CCGG C^CGG
MaeI 133 CTAG C^TAG
MaeII 675 ACGT A^CGT
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 706 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 360 CAGCTG C[AC]G^C[GT]G
MspI 167 CCGG C^CGG
NdeI 384 CATATG CA^TATG
NspI 474 ACATGT [GA]CATG^[CT]
PpuMI 89 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 360 CAGCTG CAG^CTG
SfaNI 188 GCATC GCATC(5/9)
SfcI 204 CTACAG C^T[GA][CT]AG
SspBI 347 TGTACA T^GTACA
TfiI 781 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 348 GTAC GT^AC
575 GTAC
AlwI 562 GATCC GGATC(4/5)
621 GATCC
AvaII 90 GGTCC G^G[AT]CC
218 GGACC
BanII 437 GGGCTC G[GA]GC[CT]^C
635 GGGCCC
BstAPI 147 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
271 GCAGTTGCTGC
BstYI 561 AGATCC [GA]^GATC[CT]
847 AGATCT
Cac8I 233 GCAAGC GC[ACGT]^[ACGT]GC
747 GCCAGC
EaeI 2 TGGCCA [CT]^GGCC[GA]
305 TGGCCG
EcoT14I 5 CCATGG C^C[AT][AT]GG
433 CCATGG
HaeII 20 GGCGCT [GA]GCGC^[CT]
30 GGCGCT
MflI 561 AGATCC [GA]^GATC[CT]
847 AGATCT
NcoI 5 CCATGG C^CATGG
433 CCATGG
RsaI 348 GTAC GT^AC
575 GTAC
StyI 5 CCATGG C^C[AT][AT]GG
433 CCATGG
Tsp45I 163 GTCAC ^GT[GC]AC
655 GTCAC
XhoII 561 AGATCC [GA]^GATC[CT]
847 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HphI ItaI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
Tru9I TseI Tsp509I TspRI