Restriction map for
pFN21AE6636
(Circular) (Six-base) MAPSORT of: pFN21AE6636.seq Check: 3227 from: 1 to: 5563
pFN21AE6636 5563 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 278
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2208 2906 1937
Size: 271 698 4594
Fragments arranged by size:
4594 698 271
Acc65I G'GTAC_C
Cuts at: 2884 2884
Size: 5563
AclI AA'CG_TT
Cuts at: 1563 3690 4063 1563
Size: 2127 373 3063
Fragments arranged by size:
3063 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3626 5010 5278 1632
Size: 1994 1384 268 1917
Fragments arranged by size:
1994 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5366
AflIII A'CryG_T
Cuts at: 1203 2479 4462 5439 1203
Size: 1276 1983 977 1327
Fragments arranged by size:
1983 1327 1276 977
AgeI A'CCGG_T
Cuts at: 4394 4394
Size: 5563
AhdI GACnn_n'nnGTC
Cuts at: 4292 4292
Size: 5563
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5563
AlwNI CAG_nnn'CTG
Cuts at: 2282 3306 4878 2282
Size: 1024 1572 2967
Fragments arranged by size:
2967 1572 1024
ApaI G_GGCC'C
Cuts at: 2644 2644
Size: 5563
ApaLI G'TGCA_C
Cuts at: 3620 4776 3620
Size: 1156 4407
ApoI r'AATT_y
Cuts at: 1493 2695 2872 3098 3250 5359 1493
Size: 1202 177 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 226 177 152
AseI AT'TA_AT
Cuts at: 160 1032 4117 160
Size: 872 3085 1606
Fragments arranged by size:
3085 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
AvaI C'yCGr_G
Cuts at: 1870 1943 2888 1870
Size: 73 945 4545
Fragments arranged by size:
4545 945 73
BamHI G'GATC_C
Cuts at: 2893 3260 2893
Size: 367 5196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2151 2884 4339 618
Size: 325 217 627 364 733 1455 1842
Fragments arranged by size:
1842 1455 733 627 364 325 217
BanII G_rGCy'C
Cuts at: 1391 2446 2644 2882 1391
Size: 1055 198 238 4072
Fragments arranged by size:
4072 1055 238 198
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2173 928
Size: 971 274 4318
Fragments arranged by size:
4318 971 274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3754 3788 1903
Size: 34 1817 34 3678
Fragments arranged by size:
3678 1817 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 4665 3455
Size: 1210 4353
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4174 136
Size: 107 122 71 631 605 2502 1525
Fragments arranged by size:
2502 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2852 5558 2852
Size: 2706 2857
BlpI GC'TnA_GC
Cuts at: 2980 2980
Size: 5563
Bme1580I G_kGCm'C
Cuts at: 1790 2644 3624 4780 1790
Size: 854 980 1156 2573
Fragments arranged by size:
2573 1156 980 854
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2150 3294 4252
Size: 416 53 83 399 24 733 1144 958
Cuts at: 4252 442
Size: 1753
Fragments arranged by size:
1753 1144 958 733 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5563
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2483 2483
Size: 5563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4223 1239
Size: 2984 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3688 4553 4851 3688
Size: 865 298 4400
Fragments arranged by size:
4400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4226 882
Size: 617 2727 2219
Fragments arranged by size:
2727 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3354 493
Size: 711 2150 2702
Fragments arranged by size:
2702 2150 711
BsaBI GATnn'nnATC
Cuts at: 5557 5557
Size: 5563
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3370 3752 4387 275
Size: 53 83 186 936 1837 382 635 1451
Fragments arranged by size:
1837 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3996 4394 4668 4815 5226 3996
Size: 398 274 147 411 4333
Fragments arranged by size:
4333 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5338
Fragments arranged by size:
5338 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5563
BseYI C'CCAG_C
Cuts at: 2041 2121 2367 2843 4766 5262 2041
Size: 80 246 476 1923 496 2342
Fragments arranged by size:
2342 1923 496 476 246 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 1163
Size: 573 609 4381
Fragments arranged by size:
4381 609 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3774 4802 1540
Size: 275 186 1012 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 3624 3709 4780 2163
Size: 1461 85 1071 2946
Fragments arranged by size:
2946 1461 1071 85
BsmI GAATG_Cn'
Cuts at: 3074 3167 3074
Size: 93 5470
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2446 2644 2882 3624 3709 4780
Size: 399 373 283 198 238 742 85 1071
Cuts at: 4780 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 373 283 238 198
85
BspEI T'CCGG_A
Cuts at: 5226 5226
Size: 5563
BspHI T'CATG_A
Cuts at: 3452 5182 3452
Size: 1730 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2918 844
Size: 2074 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3450 1394
Size: 429 1627 3507
Fragments arranged by size:
3507 1627 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2678 4058 4232 65
Size: 1183 1430 1380 174 1396
Fragments arranged by size:
1430 1396 1380 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2171 4207 4394 1700
Size: 110 361 2036 187 2869
Fragments arranged by size:
2869 2036 361 187 110
BsrGI T'GTAC_A
Cuts at: 96 2352 96
Size: 2256 3307
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5563
BssSI C'ACGA_G
Cuts at: 3623 4635 3623
Size: 1012 4551
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2158 2282 794
Size: 1364 124 4075
Fragments arranged by size:
4075 1364 124
BstBI TT'CG_AA
Cuts at: 4367 4367
Size: 5563
BstEII G'GTnAC_C
Cuts at: 1184 2167 1184
Size: 983 4580
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5563
BstYI r'GATC_y
Cuts at: 1972 2566 2852 2893 3260 3645 3662 5114 5200
Size: 594 286 41 367 385 17 1452 86
Cuts at: 5200 5212 5558 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 594 385 367 346 286 86
41 17 12
Bsu36I CC'TnA_GG
Cuts at: 2685 2685
Size: 5563
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2219 2438 2859 513
Size: 552 939 6 209 219 421 3217
Fragments arranged by size:
3217 939 552 421 219 209 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2179 487
Size: 683 56 953 3871
Fragments arranged by size:
3871 953 683 56
BtsI GCAGTG_nn'
Cuts at: 2051 3079 3873 3893 2051
Size: 1028 794 20 3721
Fragments arranged by size:
3721 1028 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5528
DraI TTT'AAA
Cuts at: 2867 2867
Size: 5563
DraIII CAC_nnn'GTG
Cuts at: 2158 2158
Size: 5563
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4570 809
Size: 507 3254 1802
Fragments arranged by size:
3254 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2310 3010
Size: 54 1425 50 275 18 177 303 700
Cuts at: 3010 3899 5528 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 700 303 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 1537
Size: 275 1198 4090
Fragments arranged by size:
4090 1198 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3493 1353
Size: 2140 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4150 4524 4670 387
Size: 1064 138 2561 374 146 1280
Fragments arranged by size:
2561 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3626 4223 5010 5278 1239
Size: 393 1994 597 787 268 1524
Fragments arranged by size:
1994 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2095 2640 2641 2095
Size: 545 1 5017
Fragments arranged by size:
5017 545 1
EcoRI G'AATT_C
Cuts at: 2872 3250 2872
Size: 378 5185
FspI TGC'GCA
Cuts at: 4069 4069
Size: 5563
HaeII r_GCGC'y
Cuts at: 1678 2029 2039 4710 1678
Size: 351 10 2671 2531
Fragments arranged by size:
2671 2531 351 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4204 4236 4278 4310 1373
Size: 32 135 32 2632 32 42 32 2626
Fragments arranged by size:
2632 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 2907 3153 669
Size: 988 281 397 572 246 3079
Fragments arranged by size:
3079 988 572 397 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5563
HpaI GTT'AAC
Cuts at: 2335 3153 2335
Size: 818 4745
KpnI G_GTAC'C
Cuts at: 2888 2888
Size: 5563
MfeI C'AATT_G
Cuts at: 3162 3162
Size: 5563
MluI A'CGCG_T
Cuts at: 5439 5439
Size: 5563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2353 4677 4861 1305
Size: 1048 2324 184 2007
Fragments arranged by size:
2324 2007 1048 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3564
Fragments arranged by size:
3564 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2715 3521 3880 4039 518
Size: 648 843 706 806 359 159 2042
Fragments arranged by size:
2042 843 806 706 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2367 2979 3656 4804 5049 1606
Size: 761 612 677 1148 245 2120
Fragments arranged by size:
2120 1148 761 677 612 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2010 2438 2859 513
Size: 552 939 6 428 421 3217
Fragments arranged by size:
3217 939 552 428 421 6
NdeI CA'TA_TG
Cuts at: 387 2390 387
Size: 2003 3560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5563
NotI GC'GGCC_GC
Cuts at: 3010 3010
Size: 5563
NspI r_CATG'y
Cuts at: 1132 1229 2483 2921 4466 1132
Size: 97 1254 438 1545 2229
Fragments arranged by size:
2229 1545 1254 438 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5563
PciI A'CATG_T
Cuts at: 2479 4462 2479
Size: 1983 3580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5563
PmeI GTTT'AAAC
Cuts at: 2867 2867
Size: 5563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 5145 5177 3632
Size: 32 1481 32 4018
Fragments arranged by size:
4018 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2095
Size: 5563
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5563
PsiI TTA'TAA
Cuts at: 3133 3133
Size: 5563
PspOMI G'GGCC_C
Cuts at: 2640 2640
Size: 5563
PstI C_TGCA'G
Cuts at: 830 2915 830
Size: 2085 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5563
PvuII CAG'CTG
Cuts at: 2367 2367
Size: 5563
SalI G'TCGA_C
Cuts at: 1936 2905 1936
Size: 969 4594
SbfI CC_TGCA'GG
Cuts at: 2915 2915
Size: 5563
ScaI AGT'ACT
Cuts at: 3811 5523 3811
Size: 1712 3851
SfcI C'TryA_G
Cuts at: 826 1045 2209 2911 4046 4727 4918 826
Size: 219 1164 702 1135 681 191 1471
Fragments arranged by size:
1471 1164 1135 702 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5563
SmaI CCC'GGG
Cuts at: 1872 2890 1872
Size: 1018 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3667 4568 4830 820
Size: 197 926 1724 901 262 1553
Fragments arranged by size:
1724 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3354 493
Size: 2861 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5563
SphI G_CATG'C
Cuts at: 1132 2921 1132
Size: 1789 3774
SspI AAT'ATT
Cuts at: 5 52 3487 5
Size: 47 3435 2081
Fragments arranged by size:
3435 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5563
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2438 2859 3002 513
Size: 552 939 6 428 421 143 3074
Fragments arranged by size:
3074 939 552 428 421 143 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2169 3590 3607 3760 3945 4390 325
Size: 1255 589 1421 17 153 185 445 1498
Fragments arranged by size:
1498 1421 1255 589 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 2579 3809
Size: 274 80 33 51 1443 375 227 1230
Cuts at: 3809 5521 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1230 375 274 227 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5523 5555 5523
Size: 32 5531
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5563
XbaI T'CTAG_A
Cuts at: 2899 2899
Size: 5563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5563
XmaI C'CCGG_G
Cuts at: 1870 2888 1870
Size: 1018 4545
XmnI GAAnn'nnTTC
Cuts at: 3692 3692
Size: 5563
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 276
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI