Restriction map for
pFN21AE6636
(Linear) (Six-base) MAPSORT of: KIEE6636.seq Check: 8518 from: 1 to: 858
KIEE6636.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AccI GT'mk_AC
Cuts at: 0 203 858
Size: 203 655
AflIII A'CryG_T
Cuts at: 0 474 858
Size: 474 384
AlwNI CAG_nnn'CTG
Cuts at: 0 277 858
Size: 277 581
ApaI G_GGCC'C
Cuts at: 0 639 858
Size: 639 219
ApoI r'AATT_y
Cuts at: 0 690 858
Size: 690 168
BanI G'GyrC_C
Cuts at: 0 146 858
Size: 146 712
BanII G_rGCy'C
Cuts at: 0 441 639 858
Size: 441 198 219
Fragments arranged by size:
441 219 198
BbsI GAAGACnn'nnnn_
Cuts at: 0 168 858
Size: 168 690
BglII A'GATC_T
Cuts at: 0 847 858
Size: 847 11
Bme1580I G_kGCm'C
Cuts at: 0 639 858
Size: 639 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 145 858
Size: 145 713
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 478 858
Size: 478 380
BseYI C'CCAG_C
Cuts at: 0 36 116 362 838 858
Size: 36 80 246 476 20
Fragments arranged by size:
476 246 80 36 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 858
Size: 340 518
BsiHKAI G_wGCw'C
Cuts at: 0 158 858
Size: 158 700
Bsp1286I G_dGCh'C
Cuts at: 0 158 441 639 858
Size: 158 283 198 219
Fragments arranged by size:
283 219 198 158
BsrDI GCAATG_nn'
Cuts at: 0 673 858
Size: 673 185
BsrFI r'CCGG_y
Cuts at: 0 166 858
Size: 166 692
BsrGI T'GTAC_A
Cuts at: 0 347 858
Size: 347 511
BstAPI GCAn_nnn'nTGC
Cuts at: 0 153 277 858
Size: 153 124 581
Fragments arranged by size:
581 153 124
BstEII G'GTnAC_C
Cuts at: 0 162 858
Size: 162 696
BstYI r'GATC_y
Cuts at: 0 561 847 858
Size: 561 286 11
Fragments arranged by size:
561 286 11
Bsu36I CC'TnA_GG
Cuts at: 0 680 858
Size: 680 178
BtgI C'CryG_G
Cuts at: 0 5 214 433 858
Size: 5 209 219 425
Fragments arranged by size:
425 219 209 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 174 858
Size: 174 684
BtsI GCAGTG_nn'
Cuts at: 0 46 858
Size: 46 812
DraIII CAC_nnn'GTG
Cuts at: 0 153 858
Size: 153 705
EaeI y'GGCC_r
Cuts at: 0 2 305 858
Size: 2 303 553
Fragments arranged by size:
553 303 2
EcoO109I rG'GnC_Cy
Cuts at: 0 90 635 636 858
Size: 90 545 1 222
Fragments arranged by size:
545 222 90 1
HaeII r_GCGC'y
Cuts at: 0 24 34 858
Size: 24 10 824
Fragments arranged by size:
824 24 10
HincII GTy'rAC
Cuts at: 0 330 858
Size: 330 528
HpaI GTT'AAC
Cuts at: 0 330 858
Size: 330 528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 858
Size: 348 510
MscI TGG'CCA
Cuts at: 0 4 858
Size: 4 854
MslI CAynn'nnrTG
Cuts at: 0 710 858
Size: 710 148
MspA1I CmG'CkG
Cuts at: 0 362 858
Size: 362 496
NcoI C'CATG_G
Cuts at: 0 5 433 858
Size: 5 428 425
Fragments arranged by size:
428 425 5
NdeI CA'TA_TG
Cuts at: 0 385 858
Size: 385 473
NspI r_CATG'y
Cuts at: 0 478 858
Size: 478 380
PciI A'CATG_T
Cuts at: 0 474 858
Size: 474 384
PpuMI rG'GwC_Cy
Cuts at: 0 90 858
Size: 90 768
PspOMI G'GGCC_C
Cuts at: 0 635 858
Size: 635 223
PvuII CAG'CTG
Cuts at: 0 362 858
Size: 362 496
SfcI C'TryA_G
Cuts at: 0 204 858
Size: 204 654
StyI C'CwwG_G
Cuts at: 0 5 433 858
Size: 5 428 425
Fragments arranged by size:
428 425 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 164 858
Size: 164 694
TatI w'GTAC_w
Cuts at: 0 347 574 858
Size: 347 227 284
Fragments arranged by size:
347 284 227
Enzymes that do cut:
AccI AflIII AlwNI ApaI ApoI BanI BanII BbsI
BglII Bme1580I BmrI BplI BseYI BsgI BsiHKAI Bsp1286I
BsrDI BsrFI BsrGI BstAPI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EcoO109I HaeII HincII HpaI
MmeI MscI MslI MspA1I NcoI NdeI NspI PciI
PpuMI PspOMI PvuII SfcI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BssHII BssSI BstBI BstXI BstZ17I ClaI CspCI DraI
DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI