################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccIII AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspMI BsrBI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI TaqI Tth111I TthHB8I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 1438 GTCTAC GT^[AC][GT]AC AciI 108 GCGG CCGC(-3/-1) AclI 637 AACGTT AA^CGTT ApaI 3 GGGCCC GGGCC^C BalI 1160 TGGCCA TGG^CCA BamHI 992 GGATCC G^GATCC BanII 3 GGGCCC G[GA]GC[CT]^C BbsI 1489 GTCTTC GAAGAC(2/6) BglI 529 GCCCGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1136 AGATCT A^GATCT BlpI 518 GCTCAGC GC^T[ACGT]AGC BpmI 871 CTGGAG CTGGAG(16/14) Bpu1102I 518 GCTCAGC GC^T[ACGT]AGC BpuAI 1489 GTCTTC GAAGAC(2/6) BseRI 476 CTCCTC GAGGAG(10/8) Bsp1407I 680 TGTACA T^GTACA BsrDI 489 CATTGC GCAATG(2/0) BsrFI 32 GCCGGC [GA]^CCGG[CT] BsrGI 680 TGTACA T^GTACA BstAPI 367 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 167 GGTCACC G^GT[ACGT]ACC BstPI 167 GGTCACC G^GT[ACGT]ACC CelII 518 GCTCAGC GC^T[ACGT]AGC Cfr10I 32 GCCGGC [GA]^CCGG[CT] EaeI 1160 TGGCCA [CT]^GGCC[GA] EcoO65I 167 GGTCACC G^GT[ACGT]ACC EcoT14I 901 CCTTGG C^C[AT][AT]GG FauI 108 GCGGG CCCGC(4/6) HgaI 70 GACGC GACGC(5/10) MluNI 1160 TGGCCA TGG^CCA MscI 1160 TGGCCA TGG^CCA MspA1I 1494 CAGCTG C[AC]G^C[GT]G NaeI 32 GCCGGC GCC^GGC NgoMIV 32 GCCGGC G^CCGGC PflMI 996 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 637 AACGTT AA^CGTT PvuII 1494 CAGCTG CAG^CTG ScaI 130 AGTACT AGT^ACT SpeI 1401 ACTAGT A^CTAGT SphI 763 GCATGC GCATG^C SspBI 680 TGTACA T^GTACA SspI 823 AATATT AAT^ATT StyI 901 CCTTGG C^C[AT][AT]GG Van91I 996 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 65 CGCG CG^CG 67 CGCG AcsI 136 AAATTT [GA]^AATT[CT] 451 AAATTC AlwNI 1205 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1551 CAGAAGCTG ApoI 136 AAATTT [GA]^AATT[CT] 451 AAATTC AvaII 89 GGACC G^G[AT]CC 1075 GGTCC BmyI 3 GGGCCC G[AGT]GC[ACT]^C 959 GTGCCC BsgI 27 GTGCAG GTGCAG(16/14) 82 GTGCAG BsiYI 45 CCGGGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 996 CCAAGAAATGG BslI 45 CCGGGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 996 CCAAGAAATGG BsmFI 470 GGGAC GGGAC(10/14) 778 GGGAC Bsp1286I 3 GGGCCC G[AGT]GC[ACT]^C 959 GTGCCC BspLU11I 552 ACATGT A^CATGT 1068 ACATGT BstUI 65 CGCG CG^CG 67 CGCG HphI 169 TCACC GGTGA(8/7) 1350 TCACC MaeII 23 ACGT A^CGT 638 ACGT MvnI 65 CGCG CG^CG 67 CGCG TfiI 606 GATTC G^A[AT]TC 1021 GAATC XbaI 609 TCTAGA T^CTAGA 1017 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AfaI AflIII AluI AlwI BbvI BcnI BfaI BsaJI BsmAI BsmI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI Tru9I TseI Tsp45I Tsp509I TspRI XhoII