(Circular) (Six-base) MAPSORT of: pFN21AE3227.seq Check: 8522 from: 1 to: 6265 pFN21AE3227 6265 bp With 182 enzymes: SgfI * March 28, 2012 14:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3444 3608 1937 Size: 1507 164 4594 Fragments arranged by size: 4594 1507 164 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 2643 4392 4765 1563 Size: 1080 1749 373 3063 Fragments arranged by size: 3063 1749 1080 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3168 3363 3481 4328 5712 5980 1632 Size: 1536 195 118 847 1384 268 1917 Fragments arranged by size: 1917 1536 1384 847 268 195 118 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 2028 2557 3073 5164 6141 1203 Size: 825 529 516 2091 977 1327 Fragments arranged by size: 2091 1327 977 825 529 516 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2467 1184 Size: 1283 4982 AlwNI CAG_nnn'CTG Cuts at: 3215 3561 4008 5580 3215 Size: 346 447 1572 3900 Fragments arranged by size: 3900 1572 447 346 ApaI G_GGCC'C Cuts at: 2012 2012 Size: 6265 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 2141 2456 3574 3800 3952 6061 1493 Size: 648 315 1118 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 648 315 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3590 1870 Size: 73 1647 4545 Fragments arranged by size: 4545 1647 73 BamHI G'GATC_C Cuts at: 2997 3595 3962 2997 Size: 598 367 5300 Fragments arranged by size: 5300 598 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3586 5041 618 Size: 325 217 627 1799 1455 1842 Fragments arranged by size: 1842 1799 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 3584 1391 Size: 621 1572 4072 Fragments arranged by size: 4072 1572 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3487 928 Size: 971 1588 3706 Fragments arranged by size: 3706 1588 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2540 4876 136 Size: 107 122 71 631 605 868 2336 1525 Fragments arranged by size: 2336 1525 868 631 605 122 107 71 BglII A'GATC_T Cuts at: 3141 6260 3141 Size: 3119 3146 BlpI GC'TnA_GC Cuts at: 2524 3682 2524 Size: 1158 5107 Bme1580I G_kGCm'C Cuts at: 1790 2012 2968 4326 5482 1790 Size: 222 956 1358 1156 2573 Fragments arranged by size: 2573 1358 1156 956 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2315 3540 3996 Size: 416 53 83 399 24 898 1225 456 Cuts at: 3996 4954 442 Size: 958 1753 Fragments arranged by size: 1753 1225 958 898 456 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2897 4925 1239 Size: 1658 2028 2579 Fragments arranged by size: 2579 2028 1658 Bpu10I CC'TnA_GC Cuts at: 2692 2692 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2447 2469 2488 4390 5255 5553 2447 Size: 22 19 1902 865 298 3159 Fragments arranged by size: 3159 1902 865 298 22 19 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6040 Fragments arranged by size: 6040 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2472 1186 Size: 1286 4979 BseYI C'CCAG_C Cuts at: 2056 2472 5468 5964 2056 Size: 416 2996 496 2357 Fragments arranged by size: 2996 2357 496 416 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2053 2108 1163 Size: 573 317 55 5320 Fragments arranged by size: 5320 573 317 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4326 4411 5482 4326 Size: 85 1071 5109 Fragments arranged by size: 5109 1071 85 BsmI GAATG_Cn' Cuts at: 2321 2547 2817 2887 3399 3776 3869 2321 Size: 226 270 70 512 377 93 4717 Fragments arranged by size: 4717 512 377 270 226 93 70 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2968 3584 4326 4411 5482 1391 Size: 399 222 956 616 742 85 1071 2174 Fragments arranged by size: 2174 1071 956 742 616 399 222 85 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3620 844 Size: 2776 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4152 1394 Size: 429 2329 3507 Fragments arranged by size: 3507 2329 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2493 4760 4934 65 Size: 1183 1245 2267 174 1396 Fragments arranged by size: 2267 1396 1245 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 4909 5096 1700 Size: 110 227 2872 187 2869 Fragments arranged by size: 2872 2869 227 187 110 BsrGI T'GTAC_A Cuts at: 96 2685 96 Size: 2589 3676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 2378 794 Size: 1584 4681 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 2172 1184 Size: 988 5277 BstYI r'GATC_y Cuts at: 1972 2997 3036 3141 3595 3962 4347 4364 5816 Size: 1025 39 105 454 367 385 17 1452 Cuts at: 5816 5902 5914 6260 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1025 454 385 367 346 105 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4774 Fragments arranged by size: 4774 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2710 487 Size: 683 56 1484 4042 Fragments arranged by size: 4042 1484 683 56 BtsI GCAGTG_nn' Cuts at: 2239 2535 2583 2852 3781 4575 4595 2239 Size: 296 48 269 929 794 20 3909 Fragments arranged by size: 3909 929 794 296 269 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3165 3712 4601 Size: 54 1425 50 275 18 1335 547 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1335 889 547 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4195 1353 Size: 2842 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2897 3168 3363 3481 4328 4925 5712 Size: 393 1265 271 195 118 847 597 787 Cuts at: 5712 5980 1239 Size: 268 1524 Fragments arranged by size: 1524 1265 847 787 597 393 271 268 195 118 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2057 2120 2224 5412 1678 Size: 379 63 104 3188 2531 Fragments arranged by size: 3188 2531 379 104 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 2855 2887 4906 Size: 32 135 32 770 32 481 32 2019 Cuts at: 4906 4938 4980 5012 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2019 770 481 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5379 5563 1305 Size: 4074 184 2007 Fragments arranged by size: 4074 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3167 10 Size: 54 1425 343 1335 3108 Fragments arranged by size: 3108 1425 1335 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2773 2843 2961 3051 3186 3372 4223 Size: 648 1607 70 118 90 135 186 851 Cuts at: 4223 4582 4741 518 Size: 359 159 2042 Fragments arranged by size: 2042 1607 851 648 359 186 159 135 118 90 70 MspA1I CmG'CkG Cuts at: 1606 3501 3681 4358 5506 5751 1606 Size: 1895 180 677 1148 245 2120 Fragments arranged by size: 2120 1895 1148 677 245 180 NaeI GCC'GGC Cuts at: 2039 2039 Size: 6265 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4774 Fragments arranged by size: 4774 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NgoMIV G'CCGG_C Cuts at: 2037 2037 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2561 2772 2848 3077 3623 5168 1132 Size: 97 1332 211 76 229 546 1545 2229 Fragments arranged by size: 2229 1545 1332 546 229 211 97 76 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 2557 3073 5164 2557 Size: 516 2091 3658 Fragments arranged by size: 3658 2091 516 PflMI CCAn_nnn'nTGG Cuts at: 1108 3007 1108 Size: 1899 4366 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2507 2539 4334 4366 5847 5879 2507 Size: 32 1795 32 1481 32 2893 Fragments arranged by size: 2893 1795 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 2355 3835 2355 Size: 1480 4785 PspOMI G'GGCC_C Cuts at: 2008 2008 Size: 6265 PstI C_TGCA'G Cuts at: 830 3617 830 Size: 2787 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 3501 3501 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 2137 4513 6225 2137 Size: 2376 1712 2177 Fragments arranged by size: 2376 2177 1712 SfcI C'TryA_G Cuts at: 826 1045 2178 2710 2796 3613 4748 5429 5620 Size: 219 1133 532 86 817 1135 681 191 Cuts at: 5620 826 Size: 1471 Fragments arranged by size: 1471 1135 1133 817 681 532 219 191 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2426 2467 2484 4369 5270 5532 Size: 197 926 483 41 17 1885 901 262 Cuts at: 5532 820 Size: 1553 Fragments arranged by size: 1885 1553 926 901 483 262 197 41 17 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 3406 152 Size: 3254 3011 SphI G_CATG'C Cuts at: 1132 2772 3623 1132 Size: 1640 851 3774 Fragments arranged by size: 3774 1640 851 SspI AAT'ATT Cuts at: 5 52 2830 4189 5 Size: 47 2778 1359 2081 Fragments arranged by size: 2778 2081 1359 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 2004 2906 3704 513 Size: 552 939 902 798 3074 Fragments arranged by size: 3074 939 902 798 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4292 4309 4462 4647 5092 325 Size: 1255 2712 17 153 185 445 1498 Fragments arranged by size: 2712 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2135 2685 3196 Size: 274 80 33 51 1443 158 550 511 Cuts at: 3196 4511 6223 96 Size: 1315 1712 138 Fragments arranged by size: 1712 1443 1315 550 511 274 158 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 2614 3022 3601 2614 Size: 408 579 5278 Fragments arranged by size: 5278 579 408 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI