(Linear) (Six-base) MAPSORT of: KIEE3227.seq Check: 2560 from: 1 to: 1560 KIEE3227.orf With 182 enzymes: SgfI * March 22, 2012 16:23 .. AccI GT'mk_AC Cuts at: 0 1439 1560 Size: 1439 121 AclI AA'CG_TT Cuts at: 0 638 1560 Size: 638 922 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1163 1358 1476 1560 Size: 1163 195 118 84 Fragments arranged by size: 1163 195 118 84 AflIII A'CryG_T Cuts at: 0 23 552 1068 1560 Size: 23 529 516 492 Fragments arranged by size: 529 516 492 23 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 462 1560 Size: 462 1098 AlwNI CAG_nnn'CTG Cuts at: 0 1210 1556 1560 Size: 1210 346 4 Fragments arranged by size: 1210 346 4 ApaI G_GGCC'C Cuts at: 0 7 1560 Size: 7 1553 ApoI r'AATT_y Cuts at: 0 136 451 1560 Size: 136 315 1109 Fragments arranged by size: 1109 315 136 BamHI G'GATC_C Cuts at: 0 992 1560 Size: 992 568 BanII G_rGCy'C Cuts at: 0 7 1560 Size: 7 1553 BbsI GAAGACnn'nnnn_ Cuts at: 0 1482 1560 Size: 1482 78 BglI GCCn_nnn'nGGC Cuts at: 0 535 1560 Size: 535 1025 BglII A'GATC_T Cuts at: 0 1136 1560 Size: 1136 424 BlpI GC'TnA_GC Cuts at: 0 519 1560 Size: 519 1041 Bme1580I G_kGCm'C Cuts at: 0 7 963 1560 Size: 7 956 597 Fragments arranged by size: 956 597 7 BmrI ACTGGGnnnn_n' Cuts at: 0 310 1535 1560 Size: 310 1225 25 Fragments arranged by size: 1225 310 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 892 1560 Size: 892 668 Bpu10I CC'TnA_GC Cuts at: 0 687 1560 Size: 687 873 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 464 483 1560 Size: 442 22 19 1077 Fragments arranged by size: 1077 442 22 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 467 1560 Size: 467 1093 BseYI C'CCAG_C Cuts at: 0 51 467 1560 Size: 51 416 1093 Fragments arranged by size: 1093 416 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 48 103 1560 Size: 48 55 1457 Fragments arranged by size: 1457 55 48 BsmI GAATG_Cn' Cuts at: 0 316 542 812 882 1394 1560 Size: 316 226 270 70 512 166 Fragments arranged by size: 512 316 270 226 166 70 Bsp1286I G_dGCh'C Cuts at: 0 7 963 1560 Size: 7 956 597 Fragments arranged by size: 956 597 7 BsrDI GCAATG_nn' Cuts at: 0 488 1560 Size: 488 1072 BsrFI r'CCGG_y Cuts at: 0 32 1560 Size: 32 1528 BsrGI T'GTAC_A Cuts at: 0 680 1560 Size: 680 880 BstAPI GCAn_nnn'nTGC Cuts at: 0 373 1560 Size: 373 1187 BstEII G'GTnAC_C Cuts at: 0 167 1560 Size: 167 1393 BstYI r'GATC_y Cuts at: 0 992 1031 1136 1560 Size: 992 39 105 424 Fragments arranged by size: 992 424 105 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 705 1560 Size: 705 855 BtsI GCAGTG_nn' Cuts at: 0 234 530 578 847 1560 Size: 234 296 48 269 713 Fragments arranged by size: 713 296 269 234 48 EaeI y'GGCC_r Cuts at: 0 1160 1560 Size: 1160 400 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 892 1163 1358 1476 1560 Size: 892 271 195 118 84 Fragments arranged by size: 892 271 195 118 84 HaeII r_GCGC'y Cuts at: 0 52 115 219 1560 Size: 52 63 104 1341 Fragments arranged by size: 1341 104 63 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 337 369 850 882 1560 Size: 337 32 481 32 678 Fragments arranged by size: 678 481 337 32 32 MscI TGG'CCA Cuts at: 0 1162 1560 Size: 1162 398 MslI CAynn'nnrTG Cuts at: 0 768 838 956 1046 1181 1367 1560 Size: 768 70 118 90 135 186 193 Fragments arranged by size: 768 193 186 135 118 90 70 MspA1I CmG'CkG Cuts at: 0 1496 1560 Size: 1496 64 NaeI GCC'GGC Cuts at: 0 34 1560 Size: 34 1526 NgoMIV G'CCGG_C Cuts at: 0 32 1560 Size: 32 1528 NspI r_CATG'y Cuts at: 0 556 767 843 1072 1560 Size: 556 211 76 229 488 Fragments arranged by size: 556 488 229 211 76 PciI A'CATG_T Cuts at: 0 552 1068 1560 Size: 552 516 492 Fragments arranged by size: 552 516 492 PflMI CCAn_nnn'nTGG Cuts at: 0 1002 1560 Size: 1002 558 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 502 534 1560 Size: 502 32 1026 Fragments arranged by size: 1026 502 32 PsiI TTA'TAA Cuts at: 0 350 1560 Size: 350 1210 PspOMI G'GGCC_C Cuts at: 0 3 1560 Size: 3 1557 PvuII CAG'CTG Cuts at: 0 1496 1560 Size: 1496 64 ScaI AGT'ACT Cuts at: 0 132 1560 Size: 132 1428 SfcI C'TryA_G Cuts at: 0 173 705 791 1560 Size: 173 532 86 769 Fragments arranged by size: 769 532 173 86 SmlI C'TyrA_G Cuts at: 0 421 462 479 1560 Size: 421 41 17 1081 Fragments arranged by size: 1081 421 41 17 SpeI A'CTAG_T Cuts at: 0 1401 1560 Size: 1401 159 SphI G_CATG'C Cuts at: 0 767 1560 Size: 767 793 SspI AAT'ATT Cuts at: 0 825 1560 Size: 825 735 StyI C'CwwG_G Cuts at: 0 901 1560 Size: 901 659 TatI w'GTAC_w Cuts at: 0 130 680 1191 1560 Size: 130 550 511 369 Fragments arranged by size: 550 511 369 130 XbaI T'CTAG_A Cuts at: 0 609 1017 1560 Size: 609 408 543 Fragments arranged by size: 609 543 408 Enzymes that do cut: AccI AclI AcuI AflIII AlfI AlwNI ApaI ApoI BamHI BanII BbsI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BseRI BseYI BsgI BsmI Bsp1286I BsrDI BsrFI BsrGI BstAPI BstEII BstYI BtgZI BtsI EaeI Eco57MI HaeII Hin4I MscI MslI MspA1I NaeI NgoMIV NspI PciI PflMI PpiI PsiI PspOMI PvuII ScaI SfcI SmlI SpeI SphI SspI StyI TatI XbaI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpuMI PshAI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI StuI SwaI TaqII TstI Tth111I XcmI XhoI XmaI XmnI ZraI