Restriction map for
pFN21AE4141
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbsI BbvCI BclI BcnI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 699 GTAC GT^AC
AflIII 946 ACATGT A^C[GA][CT]GT
AocI 713 CCTTAGG CC^T[ACGT]AGG
AspHI 1377 GTGCTC G[AT]GC[AT]^C
BalI 1266 TGGCCA TGG^CCA
BamHI 876 GGATCC G^GATCC
BanI 1504 GGTGCC G^G[CT][GA]CC
BanII 1139 GGGCTC G[GA]GC[CT]^C
BcgI 643 CGAATTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1543 GGATAC GTATCC(6/5)
BglI 1268 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1596 AGATCT A^GATCT
BsaI 1643 GGTCTC GGTCTC(1/5)
BsiHKAI 1377 GTGCTC G[AT]GC[AT]^C
BsmAI 1644 GTCTC GTCTC(1/5)
BspLU11I 946 ACATGT A^CATGT
BsrDI 190 GCAATG GCAATG(2/0)
Bsu36I 713 CCTTAGG CC^T[ACGT]AGG
DraII 954 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1011 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1266 TGGCCA [CT]^GGCC[GA]
Eco81I 713 CCTTAGG CC^T[ACGT]AGG
EcoNI 327 CCTGCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 954 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 479 GAATTC G^AATTC
EcoRV 1324 GATATC GAT^ATC
EcoT14I 1097 CCTTGG C^C[AT][AT]GG
MluNI 1266 TGGCCA TGG^CCA
MscI 1266 TGGCCA TGG^CCA
MslI 1648 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 870 CATATG CA^TATG
NspI 946 ACATGT [GA]CATG^[CT]
PflMI 1363 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 954 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 200 CAGCTG CAG^CTG
RsaI 699 GTAC GT^AC
SmlI 331 CTTGAG C^T[CT][GA]AG
StyI 1097 CCTTGG C^C[AT][AT]GG
Van91I 1363 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 607 CCAGTTATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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Asp700I 517 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
541 GAAAAGTTTC
AvaII 809 GGTCC G^G[AT]CC
955 GGACC
BfaI 460 CTAG C^TAG
1408 CTAG
BmyI 1139 GGGCTC G[AGT]GC[ACT]^C
1377 GTGCTC
BsaBI 685 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1591 GATCCAGATC
BsaJI 926 CCAGGG C^C[ACGT][ACGT]GG
1097 CCTTGG
BsgI 1290 CTGCAC GTGCAG(16/14)
1088 GTGCAG
BsiYI 327 CCTGCTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1363 CCAGAATATGG
BslI 327 CCTGCTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1363 CCAGAATATGG
Bsp1286I 1139 GGGCTC G[AGT]GC[ACT]^C
1377 GTGCTC
BspHI 627 TCATGA T^CATGA
1514 TCATGA
BsrBI 756 CCGCTC CCGCTC(-3/-3)
379 GAGCGG
BssKI 926 CCAGG ^CC[ACGT]GG
1073 CCAGG
BstNI 926 CCAGG CC^[AT]GG
1073 CCAGG
Cfr13I 809 GGTCC G^G[ACGT]CC
955 GGACC
DraI 220 TTTAAA TTT^AAA
1173 TTTAAA
EarI 116 CTCTTC CTCTTC(1/4)
1150 GAAGAG
EcoRII 926 CCAGG ^CC[AT]GG
1073 CCAGG
Ksp632I 116 CTCTTC CTCTTC(1/4)
1150 GAAGAG
MaeI 460 CTAG C^TAG
1408 CTAG
MaeII 1256 ACGT A^CGT
1564 ACGT
MamI 685 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1591 GATCCAGATC
MspA1I 200 CAGCTG C[AC]G^C[GT]G
338 CAGCGG
MvaI 926 CCAGG CC^[AT]GG
1073 CCAGG
RcaI 627 TCATGA T^CATGA
1514 TCATGA
Sau96I 809 GGTCC G^G[ACGT]CC
955 GGACC
ScrFI 926 CCAGG CC^[ACGT]GG
1073 CCAGG
StuI 267 AGGCCT AGG^CCT
717 AGGCCT
TaqI 474 TCGA T^CGA
642 TCGA
TthHB8I 474 TCGA T^CGA
642 TCGA
XmnI 517 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
541 GAAAAGTTTC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BpmI BsmI BsrI BstF5I
BstYI Cac8I DdeI DpnII EcoT22I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MwoI NdeII NlaIII
NlaIV NsiI Sau3AI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII