(Circular) (Six-base) MAPSORT of: pFN21AE4141.seq Check: 3994 from: 1 to: 6379 pFN21AE4141 6379 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3722 1937 Size: 1785 4594 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 2118 3560 4442 5826 6094 1632 Size: 476 10 1442 882 1384 268 1917 Fragments arranged by size: 1917 1442 1384 882 476 268 10 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6182 AflIII A'CryG_T Cuts at: 1203 2951 5278 6255 1203 Size: 1748 2327 977 1327 Fragments arranged by size: 2327 1748 1327 977 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 5108 5108 Size: 6379 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2454 1184 Size: 1270 5109 AlwNI CAG_nnn'CTG Cuts at: 4122 5694 4122 Size: 1572 4807 ApaLI G'TGCA_C Cuts at: 4436 5592 4436 Size: 1156 5223 ApoI r'AATT_y Cuts at: 1493 2222 2444 2484 2525 3688 3914 4066 6175 Size: 729 222 40 41 1163 226 152 2109 Cuts at: 6175 1493 Size: 1697 Fragments arranged by size: 2109 1697 1163 729 226 222 152 41 40 AseI AT'TA_AT Cuts at: 160 1032 4933 160 Size: 872 3901 1606 Fragments arranged by size: 3901 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 3704 1870 Size: 73 1761 4545 Fragments arranged by size: 4545 1761 73 BamHI G'GATC_C Cuts at: 2881 3709 4076 2881 Size: 828 367 5184 Fragments arranged by size: 5184 828 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3509 3700 5155 618 Size: 325 217 627 1722 191 1455 1842 Fragments arranged by size: 1842 1722 1455 627 325 217 191 BanII G_rGCy'C Cuts at: 1391 3148 3698 1391 Size: 1757 550 4072 Fragments arranged by size: 4072 1757 550 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5408 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2637 2671 4570 4604 1903 Size: 34 700 34 1899 34 3678 Fragments arranged by size: 3678 1899 700 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4271 5481 3542 Size: 729 1210 4440 Fragments arranged by size: 4440 1210 729 BclI T'GATC_A Cuts at: 1548 1548 Size: 6379 BfrBI ATG'CAT Cuts at: 2078 2288 2794 2078 Size: 210 506 5663 Fragments arranged by size: 5663 506 210 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3279 4990 136 Size: 107 122 71 631 605 1607 1711 1525 Fragments arranged by size: 1711 1607 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3601 6374 3601 Size: 2773 3606 BlpI GC'TnA_GC Cuts at: 3796 3796 Size: 6379 Bme1580I G_kGCm'C Cuts at: 1790 4440 5596 1790 Size: 2650 1156 2573 Fragments arranged by size: 2650 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2988 3630 4110 Size: 416 53 83 399 24 1571 642 480 Cuts at: 4110 5068 442 Size: 958 1753 Fragments arranged by size: 1753 1571 958 642 480 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3132 3340 3564 5039 1239 Size: 1893 208 224 1475 2579 Fragments arranged by size: 2579 1893 1475 224 208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2357 4504 5369 5667 2357 Size: 2147 865 298 3069 Fragments arranged by size: 3069 2147 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3654 5042 882 Size: 617 2155 1388 2219 Fragments arranged by size: 2219 2155 1388 617 BsaAI yAC'GTr Cuts at: 493 1204 4170 493 Size: 711 2966 2702 Fragments arranged by size: 2966 2702 711 BsaBI GATnn'nnATC Cuts at: 2694 3600 6373 2694 Size: 906 2773 2700 Fragments arranged by size: 2773 2700 906 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4186 4568 5203 275 Size: 53 83 186 936 2653 382 635 1451 Fragments arranged by size: 2653 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4812 5210 5484 5631 6042 4812 Size: 398 274 147 411 5149 Fragments arranged by size: 5149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6154 Fragments arranged by size: 6154 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6379 BseYI C'CCAG_C Cuts at: 5582 6078 5582 Size: 496 5883 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3114 3280 1163 Size: 573 1378 166 4262 Fragments arranged by size: 4262 1378 573 166 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4590 5618 1540 Size: 275 186 1828 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3386 4440 4525 5596 3386 Size: 1054 85 1071 4169 Fragments arranged by size: 4169 1071 1054 85 BsmI GAATG_Cn' Cuts at: 2078 2433 2660 3446 3890 3983 2078 Size: 355 227 786 444 93 4474 Fragments arranged by size: 4474 786 444 355 227 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3148 3386 3698 4440 4525 5596 1391 Size: 399 1358 238 312 742 85 1071 2174 Fragments arranged by size: 2174 1358 1071 742 399 312 238 85 BspEI T'CCGG_A Cuts at: 6042 6042 Size: 6379 BspHI T'CATG_A Cuts at: 2632 3519 4268 5998 2632 Size: 887 749 1730 3013 Fragments arranged by size: 3013 1730 887 749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3734 844 Size: 2890 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2386 2763 4266 1394 Size: 429 563 377 1503 3507 Fragments arranged by size: 3507 1503 563 429 377 BsrDI GCAATG_nn' Cuts at: 65 1248 2202 4874 5048 65 Size: 1183 954 2672 174 1396 Fragments arranged by size: 2672 1396 1183 954 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5023 5210 1700 Size: 110 3213 187 2869 Fragments arranged by size: 3213 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6379 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6379 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6379 BstYI r'GATC_y Cuts at: 1972 2347 2695 2757 2881 3024 3601 3709 4076 Size: 375 348 62 124 143 577 108 367 Cuts at: 4076 4461 4478 5930 6016 6028 6374 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 577 385 375 367 348 346 143 124 108 86 62 17 12 Bsu36I CC'TnA_GG Cuts at: 2719 2719 Size: 6379 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4888 Fragments arranged by size: 4888 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 3895 4689 4709 3895 Size: 794 20 5565 Fragments arranged by size: 5565 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6344 DraI TTT'AAA Cuts at: 2227 3180 3683 2227 Size: 953 503 4923 Fragments arranged by size: 4923 953 503 DraIII CAC_nnn'GTG Cuts at: 3021 3021 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5386 809 Size: 507 4070 1802 Fragments arranged by size: 4070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3271 3826 4715 Size: 54 1425 50 275 18 1441 555 889 Cuts at: 4715 6344 8 Size: 1629 43 Fragments arranged by size: 1629 1441 1425 889 555 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 1537 Size: 275 2014 4090 Fragments arranged by size: 4090 2014 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 3150 4309 1353 Size: 774 1023 1159 3423 Fragments arranged by size: 3423 1159 1023 774 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2118 3132 3340 3560 3564 4442 Size: 393 476 10 1014 208 220 4 878 Cuts at: 4442 5039 5826 6094 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1014 878 787 597 476 393 268 220 208 10 4 EcoNI CCTnn'n_nnAGG Cuts at: 2336 2336 Size: 6379 EcoO109I rG'GnC_Cy Cuts at: 2960 2960 Size: 6379 EcoRI G'AATT_C Cuts at: 2484 3688 4066 2484 Size: 1204 378 4797 Fragments arranged by size: 4797 1204 378 EcoRV GAT'ATC Cuts at: 3331 3331 Size: 6379 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2572 2572 Size: 6379 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 5526 1678 Size: 3848 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 5020 5052 5094 Size: 32 135 32 887 32 2529 32 42 Cuts at: 5094 5126 1373 Size: 32 2626 Fragments arranged by size: 2626 2529 887 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3723 3969 669 Size: 988 281 1785 246 3079 Fragments arranged by size: 3079 1785 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6379 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5493 5677 1305 Size: 4188 184 2007 Fragments arranged by size: 4188 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3273 10 Size: 54 1425 343 1441 3116 Fragments arranged by size: 3116 1441 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3657 4337 4696 4855 518 Size: 648 2491 680 359 159 2042 Fragments arranged by size: 2491 2042 680 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2207 2345 3795 4472 5620 5865 1606 Size: 601 138 1450 677 1148 245 2120 Fragments arranged by size: 2120 1450 1148 677 601 245 138 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4888 Fragments arranged by size: 4888 939 552 NdeI CA'TA_TG Cuts at: 387 2876 387 Size: 2489 3890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6379 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NsiI A_TGCA'T Cuts at: 2080 2290 2796 2080 Size: 210 506 5663 Fragments arranged by size: 5663 506 210 NspI r_CATG'y Cuts at: 1132 1229 2955 3737 5282 1132 Size: 97 1726 782 1545 2229 Fragments arranged by size: 2229 1726 1545 782 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6379 PciI A'CATG_T Cuts at: 2951 5278 2951 Size: 2327 4052 PflMI CCAn_nnn'nTGG Cuts at: 1108 3374 1108 Size: 2266 4113 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3149 3181 4448 4480 5961 5993 3149 Size: 32 1267 32 1481 32 3535 Fragments arranged by size: 3535 1481 1267 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2960 2960 Size: 6379 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6379 PsiI TTA'TAA Cuts at: 3949 3949 Size: 6379 PstI C_TGCA'G Cuts at: 830 3731 830 Size: 2901 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 PvuII CAG'CTG Cuts at: 2207 2207 Size: 6379 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 4627 6339 4627 Size: 1712 4667 SfcI C'TryA_G Cuts at: 826 1045 2354 2986 3283 3727 4862 5543 5734 Size: 219 1309 632 297 444 1135 681 191 Cuts at: 5734 826 Size: 1471 Fragments arranged by size: 1471 1309 1135 681 632 444 297 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 3706 1872 Size: 1834 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2336 4483 5384 5646 820 Size: 197 926 393 2147 901 262 1553 Fragments arranged by size: 2147 1553 926 901 393 262 197 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 2274 2724 1517 Size: 757 450 5172 Fragments arranged by size: 5172 757 450 StyI C'CwwG_G Cuts at: 513 1065 2004 3102 3818 513 Size: 552 939 1098 716 3074 Fragments arranged by size: 3074 1098 939 716 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3095 3645 4406 4423 4576 4761 5206 Size: 1255 1515 550 761 17 153 185 445 Cuts at: 5206 325 Size: 1498 Fragments arranged by size: 1515 1498 1255 761 550 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2703 4625 6337 Size: 274 80 33 51 1443 726 1922 1712 Cuts at: 6337 96 Size: 138 Fragments arranged by size: 1922 1712 1443 726 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3343 3375 6339 6371 3343 Size: 32 2964 32 3351 Fragments arranged by size: 3351 2964 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2619 2619 Size: 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 3704 1870 Size: 1834 4545 XmnI GAAnn'nnTTC Cuts at: 2526 2550 4508 2526 Size: 24 1958 4397 Fragments arranged by size: 4397 1958 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI