(Linear) (Six-base) MAPSORT of: KIEE4141.seq Check: 8605 from: 1 to: 1674 KIEE4141.orf With 182 enzymes: SgfI * July 3, 2012 18:38 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 113 1555 1674 Size: 103 10 1442 119 Fragments arranged by size: 1442 119 103 10 AflIII A'CryG_T Cuts at: 0 946 1674 Size: 946 728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 449 1674 Size: 449 1225 ApoI r'AATT_y Cuts at: 0 217 439 479 520 1674 Size: 217 222 40 41 1154 Fragments arranged by size: 1154 222 217 41 40 BamHI G'GATC_C Cuts at: 0 876 1674 Size: 876 798 BanI G'GyrC_C Cuts at: 0 1504 1674 Size: 1504 170 BanII G_rGCy'C Cuts at: 0 1143 1674 Size: 1143 531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 632 666 1674 Size: 632 34 1008 Fragments arranged by size: 1008 632 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1537 1674 Size: 1537 137 BfrBI ATG'CAT Cuts at: 0 73 283 789 1674 Size: 73 210 506 885 Fragments arranged by size: 885 506 210 73 BglI GCCn_nnn'nGGC Cuts at: 0 1274 1674 Size: 1274 400 BglII A'GATC_T Cuts at: 0 1596 1674 Size: 1596 78 BmrI ACTGGGnnnn_n' Cuts at: 0 983 1625 1674 Size: 983 642 49 Fragments arranged by size: 983 642 49 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1127 1335 1559 1674 Size: 1127 208 224 115 Fragments arranged by size: 1127 224 208 115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 1674 Size: 352 1322 BsaI GGTCTCn'nnnn_ Cuts at: 0 1649 1674 Size: 1649 25 BsaBI GATnn'nnATC Cuts at: 0 689 1595 1674 Size: 689 906 79 Fragments arranged by size: 906 689 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1109 1275 1674 Size: 1109 166 399 Fragments arranged by size: 1109 399 166 BsiHKAI G_wGCw'C Cuts at: 0 1381 1674 Size: 1381 293 BsmI GAATG_Cn' Cuts at: 0 73 428 655 1441 1674 Size: 73 355 227 786 233 Fragments arranged by size: 786 355 233 227 73 Bsp1286I G_dGCh'C Cuts at: 0 1143 1381 1674 Size: 1143 238 293 Fragments arranged by size: 1143 293 238 BspHI T'CATG_A Cuts at: 0 627 1514 1674 Size: 627 887 160 Fragments arranged by size: 887 627 160 BsrBI CCG'CTC Cuts at: 0 381 758 1674 Size: 381 377 916 Fragments arranged by size: 916 381 377 BsrDI GCAATG_nn' Cuts at: 0 197 1674 Size: 197 1477 BstYI r'GATC_y Cuts at: 0 342 690 752 876 1019 1596 1674 Size: 342 348 62 124 143 577 78 Fragments arranged by size: 577 348 342 143 124 78 62 Bsu36I CC'TnA_GG Cuts at: 0 714 1674 Size: 714 960 DraI TTT'AAA Cuts at: 0 222 1175 1674 Size: 222 953 499 Fragments arranged by size: 953 499 222 DraIII CAC_nnn'GTG Cuts at: 0 1016 1674 Size: 1016 658 EaeI y'GGCC_r Cuts at: 0 1266 1674 Size: 1266 408 EarI CTCTTCn'nnn_ Cuts at: 0 122 1145 1674 Size: 122 1023 529 Fragments arranged by size: 1023 529 122 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 113 1127 1335 1555 1559 1674 Size: 103 10 1014 208 220 4 115 Fragments arranged by size: 1014 220 208 115 103 10 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 331 1674 Size: 331 1343 EcoO109I rG'GnC_Cy Cuts at: 0 955 1674 Size: 955 719 EcoRI G'AATT_C Cuts at: 0 479 1674 Size: 479 1195 EcoRV GAT'ATC Cuts at: 0 1326 1674 Size: 1326 348 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 567 1674 Size: 567 1107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 454 486 1674 Size: 454 32 1188 Fragments arranged by size: 1188 454 32 MscI TGG'CCA Cuts at: 0 1268 1674 Size: 1268 406 MslI CAynn'nnrTG Cuts at: 0 1652 1674 Size: 1652 22 MspA1I CmG'CkG Cuts at: 0 202 340 1674 Size: 202 138 1334 Fragments arranged by size: 1334 202 138 NdeI CA'TA_TG Cuts at: 0 871 1674 Size: 871 803 NsiI A_TGCA'T Cuts at: 0 75 285 791 1674 Size: 75 210 506 883 Fragments arranged by size: 883 506 210 75 NspI r_CATG'y Cuts at: 0 950 1674 Size: 950 724 PciI A'CATG_T Cuts at: 0 946 1674 Size: 946 728 PflMI CCAn_nnn'nTGG Cuts at: 0 1369 1674 Size: 1369 305 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1144 1176 1674 Size: 1144 32 498 Fragments arranged by size: 1144 498 32 PpuMI rG'GwC_Cy Cuts at: 0 955 1674 Size: 955 719 PvuII CAG'CTG Cuts at: 0 202 1674 Size: 202 1472 SfcI C'TryA_G Cuts at: 0 349 981 1278 1674 Size: 349 632 297 396 Fragments arranged by size: 632 396 349 297 SmlI C'TyrA_G Cuts at: 0 331 1674 Size: 331 1343 StuI AGG'CCT Cuts at: 0 269 719 1674 Size: 269 450 955 Fragments arranged by size: 955 450 269 StyI C'CwwG_G Cuts at: 0 1097 1674 Size: 1097 577 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1090 1640 1674 Size: 1090 550 34 Fragments arranged by size: 1090 550 34 TatI w'GTAC_w Cuts at: 0 698 1674 Size: 698 976 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1338 1370 1674 Size: 1338 32 304 Fragments arranged by size: 1338 304 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 614 1674 Size: 614 1060 XmnI GAAnn'nnTTC Cuts at: 0 521 545 1674 Size: 521 24 1129 Fragments arranged by size: 1129 521 24 Enzymes that do cut: AcuI AflIII AlfI ApoI BamHI BanI BanII BcgI BciVI BfrBI BglI BglII BmrI BpmI BpuEI BsaI BsaBI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BstYI Bsu36I DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I MscI MslI MspA1I NdeI NsiI NspI PciI PflMI PpiI PpuMI PvuII SfcI SmlI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BclI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DrdI EagI EciI EcoICRI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI