################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccI 807 GTCTAC GT^[AC][GT]AC AccII 1621 CGCG CG^CG BalI 335 TGGCCA TGG^CCA BcnI 149 CCGGG CC^[GC]GG BglII 982 AGATCT A^GATCT BsmBI 963 GAGACG CGTCTC(1/5) BspMI 57 ACCTGC ACCTGC(4/8) BsrDI 39 CATTGC GCAATG(2/0) BstAPI 1089 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1621 CGCG CG^CG CfoI 1622 GCGC GCG^C EaeI 335 TGGCCA [CT]^GGCC[GA] EcoT14I 1012 CCAAGG C^C[AT][AT]GG HapII 149 CCGG C^CGG HgaI 1619 GACGC GACGC(5/10) HhaI 1622 GCGC GCG^C HinP1I 1622 GCGC G^CGC HpaII 149 CCGG C^CGG MluNI 335 TGGCCA TGG^CCA MscI 335 TGGCCA TGG^CCA MspI 149 CCGG C^CGG MvnI 1621 CGCG CG^CG NciI 149 CCGGG CC^[GC]GG NheI 484 GCTAGC G^CTAGC NlaIV 145 GGGACC GG[ACGT]^[ACGT]CC NspI 106 ACATGC [GA]CATG^[CT] SmlI 618 CTCAAG C^T[CT][GA]AG StyI 1012 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 499 GCGG CCGC(-3/-1) 2258 GCGG Asp700I 1025 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1316 GAAGAAATTC AvaII 146 GGACC G^G[AT]CC 1009 GGACC BbvI 481 GCAGC GCAGC(8/12) 1751 GCAGC BciVI 351 GGATAC GTATCC(6/5) 509 GGATAC BsaJI 1012 CCAAGG C^C[ACGT][ACGT]GG 2235 CCCTGG BsiYI 338 CCAACAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1040 CCAGATCAAGG BslI 338 CCAACAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1040 CCAGATCAAGG Bsp1407I 130 TGTACA T^GTACA 2196 TGTACA BspHI 1479 TCATGA T^CATGA 2223 TCATGA BsrGI 130 TGTACA T^GTACA 2196 TGTACA BsrI 804 CCAGT ACTGG(1/-1) 1192 CCAGT BstF5I 1451 GGATG GGATG(2/0) 2130 GGATG Cac8I 484 GCTAGC GC[ACGT]^[ACGT]GC 948 GCCTGC Fnu4HI 481 GCAGC GC^[ACGT]GC 1751 GCAGC FokI 1451 GGATG GGATG(9/13) 2130 GGATG HaeIII 336 GGCC GG^CC 1365 GGCC ItaI 481 GCAGC GC^[ACGT]GC 1751 GCAGC MaeII 18 ACGT A^CGT 1062 ACGT MwoI 1089 GCAAGAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1751 GCAGCAGAAGC RcaI 1479 TCATGA T^CATGA 2223 TCATGA SspBI 130 TGTACA T^GTACA 2196 TGTACA TaqI 24 TCGA T^CGA 1023 TCGA TseI 481 GCAGC G^C[AT]GC 1751 GCAGC TspRI 237 CAGTG CA[GC]TG[ACGT][ACGT]^ 2150 CAGTG TthHB8I 24 TCGA T^CGA 1023 TCGA XmnI 1025 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1316 GAAGAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AspHI BanII BbsI BclI BfaI BmyI BpuAI BsaBI BseRI BsiHKAI BsmAI BsmFI Bsp1286I BssKI BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII FbaI HinfI HphI Ksp632I MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I Tsp45I Tsp509I XhoII