(Circular) (Six-base) MAPSORT of: pFN21AE2081.seq Check: 8489 from: 1 to: 6967 pFN21AE2081 6967 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4777 278 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2813 4310 1937 Size: 876 1497 4594 Fragments arranged by size: 4594 1497 876 Acc65I G'GTAC_C Cuts at: 4288 4288 Size: 6967 AclI AA'CG_TT Cuts at: 1563 5094 5467 1563 Size: 3531 373 3063 Fragments arranged by size: 3531 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2224 2694 5030 6414 6682 1632 Size: 592 470 2336 1384 268 1917 Fragments arranged by size: 2336 1917 1384 592 470 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6770 AflIII A'CryG_T Cuts at: 1203 5866 6843 1203 Size: 4663 977 1327 Fragments arranged by size: 4663 1327 977 AgeI A'CCGG_T Cuts at: 5798 5798 Size: 6967 AhdI GACnn_n'nnGTC Cuts at: 5696 5696 Size: 6967 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6967 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3743 1184 Size: 2559 4408 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2001 2033 2001 Size: 32 6935 AlwNI CAG_nnn'CTG Cuts at: 4710 6282 4710 Size: 1572 5395 ApaLI G'TGCA_C Cuts at: 5024 6180 5024 Size: 1156 5811 ApoI r'AATT_y Cuts at: 1493 2324 2713 3325 3973 4220 4276 4502 4654 Size: 831 389 612 648 247 56 226 152 Cuts at: 4654 6763 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 831 648 612 389 247 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 5521 160 Size: 872 4489 1606 Fragments arranged by size: 4489 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 AvaI C'yCGr_G Cuts at: 1870 1943 4292 1870 Size: 73 2349 4545 Fragments arranged by size: 4545 2349 73 BamHI G'GATC_C Cuts at: 4297 4664 4297 Size: 367 6600 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4288 5743 618 Size: 325 217 627 2501 1455 1842 Fragments arranged by size: 2501 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3112 3896 3959 4286 1391 Size: 1721 784 63 327 4072 Fragments arranged by size: 4072 1721 784 327 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2298 3345 4260 928 Size: 971 399 1047 915 3635 Fragments arranged by size: 3635 1047 971 915 399 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5158 5192 1903 Size: 34 3221 34 3678 Fragments arranged by size: 3678 3221 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2350 2508 4859 6069 2350 Size: 158 2351 1210 3248 Fragments arranged by size: 3248 2351 1210 158 BclI T'GATC_A Cuts at: 1548 3595 4012 4225 1548 Size: 2047 417 213 4290 Fragments arranged by size: 4290 2047 417 213 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5578 136 Size: 107 122 71 631 605 3906 1525 Fragments arranged by size: 3906 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2987 6962 2987 Size: 3975 2992 BlpI GC'TnA_GC Cuts at: 4384 4384 Size: 6967 Bme1580I G_kGCm'C Cuts at: 1790 2105 5028 6184 1790 Size: 315 2923 1156 2573 Fragments arranged by size: 2923 2573 1156 315 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6967 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2803 4698 5656 Size: 416 53 83 399 24 1386 1895 958 Cuts at: 5656 442 Size: 1753 Fragments arranged by size: 1895 1753 1386 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2493 1056 Size: 1437 5530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5627 1239 Size: 4388 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2608 5092 5957 6255 2608 Size: 2484 865 298 3320 Fragments arranged by size: 3320 2484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5630 882 Size: 617 4131 2219 Fragments arranged by size: 4131 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4758 493 Size: 711 3554 2702 Fragments arranged by size: 3554 2702 711 BsaBI GATnn'nnATC Cuts at: 2412 3046 4056 6961 2412 Size: 634 1010 2905 2418 Fragments arranged by size: 2905 2418 1010 634 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4774 5156 5791 275 Size: 53 83 186 936 3241 382 635 1451 Fragments arranged by size: 3241 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5400 5798 6072 6219 6630 5400 Size: 398 274 147 411 5737 Fragments arranged by size: 5737 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3535 3565 1178 Size: 30 165 30 2132 30 4580 Fragments arranged by size: 4580 2132 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2274 2388 2587 2590 3069 3883 3892 1186 Size: 1088 114 199 3 479 814 9 4261 Fragments arranged by size: 4261 1088 814 479 199 114 9 3 BseYI C'CCAG_C Cuts at: 2949 6170 6666 2949 Size: 3221 496 3250 Fragments arranged by size: 3250 3221 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6394 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4417 5178 6206 1540 Size: 275 186 2416 761 1028 2301 Fragments arranged by size: 2416 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3112 3896 3959 5028 5113 6184 3112 Size: 784 63 1069 85 1071 3895 Fragments arranged by size: 3895 1071 1069 784 85 63 BsmI GAATG_Cn' Cuts at: 4478 4571 4478 Size: 93 6874 BsmBI CGTCTCn'nnnn_ Cuts at: 2962 2962 Size: 6967 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 3112 3896 3959 4286 5028 5113 Size: 399 315 1007 784 63 327 742 85 Cuts at: 5113 6184 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1007 784 742 399 327 315 85 63 BspEI T'CCGG_A Cuts at: 6630 6630 Size: 6967 BspHI T'CATG_A Cuts at: 3484 4228 4856 6586 3484 Size: 744 628 1730 3865 Fragments arranged by size: 3865 1730 744 628 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2071 4322 844 Size: 1227 2251 3489 Fragments arranged by size: 3489 2251 1227 BsrBI CCG'CTC Cuts at: 1394 1823 4854 1394 Size: 429 3031 3507 Fragments arranged by size: 3507 3031 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2043 5462 5636 65 Size: 1183 795 3419 174 1396 Fragments arranged by size: 3419 1396 1183 795 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5611 5798 1700 Size: 110 3801 187 2869 Fragments arranged by size: 3801 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2135 4201 96 Size: 2039 2066 2862 Fragments arranged by size: 2862 2066 2039 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6967 BssSI C'ACGA_G Cuts at: 5027 6039 5027 Size: 1012 5955 BstAPI GCAn_nnn'nTGC Cuts at: 794 3100 794 Size: 2306 4661 BstBI TT'CG_AA Cuts at: 5771 5771 Size: 6967 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6967 BstYI r'GATC_y Cuts at: 1972 2832 2987 3041 4148 4297 4664 5049 5066 Size: 860 155 54 1107 149 367 385 17 Cuts at: 5066 6518 6604 6616 6962 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1107 860 385 367 346 155 149 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5476 Fragments arranged by size: 5476 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6228 Fragments arranged by size: 6228 683 56 BtsI GCAGTG_nn' Cuts at: 4483 5277 5297 4483 Size: 794 20 6153 Fragments arranged by size: 6153 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6967 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6932 DraI TTT'AAA Cuts at: 4271 4271 Size: 6967 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5974 809 Size: 507 4658 1802 Fragments arranged by size: 4658 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2340 4414 5303 Size: 54 1425 50 275 18 510 2074 889 Cuts at: 5303 6932 8 Size: 1629 43 Fragments arranged by size: 2074 1629 1425 889 510 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4414 1537 Size: 275 2602 4090 Fragments arranged by size: 4090 2602 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2065 2647 2670 2756 2823 3004 3718 3799 Size: 712 582 23 86 67 181 714 81 Cuts at: 3799 4897 1353 Size: 1098 3423 Fragments arranged by size: 3423 1098 714 712 582 181 86 81 67 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5554 5928 6074 387 Size: 1064 138 3965 374 146 1280 Fragments arranged by size: 3965 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3110 3894 3957 3110 Size: 784 63 6120 Fragments arranged by size: 6120 784 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2224 2694 5030 5627 6414 6682 1239 Size: 393 592 470 2336 597 787 268 1524 Fragments arranged by size: 2336 1524 787 597 592 470 393 268 EcoRI G'AATT_C Cuts at: 4276 4654 4276 Size: 378 6589 FspI TGC'GCA Cuts at: 5473 5473 Size: 6967 HaeII r_GCGC'y Cuts at: 1678 6114 1678 Size: 4436 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2421 2453 2621 2653 2857 Size: 32 135 32 849 32 168 32 204 Cuts at: 2857 2889 3278 3310 4217 4249 5608 5640 5682 Size: 32 389 32 907 32 1359 32 42 Cuts at: 5682 5714 1373 Size: 32 2626 Fragments arranged by size: 2626 1359 907 849 389 204 168 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4311 4557 669 Size: 988 281 2373 246 3079 Fragments arranged by size: 3079 2373 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6967 HpaI GTT'AAC Cuts at: 4557 4557 Size: 6967 KpnI G_GTAC'C Cuts at: 4292 4292 Size: 6967 MfeI C'AATT_G Cuts at: 4566 4566 Size: 6967 MluI A'CGCG_T Cuts at: 6843 6843 Size: 6967 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6081 6265 1305 Size: 4776 184 2007 Fragments arranged by size: 4776 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2342 10 Size: 54 1425 343 510 4635 Fragments arranged by size: 4635 1425 510 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2406 3234 3489 4203 4925 5284 5443 Size: 648 1240 828 255 714 722 359 159 Cuts at: 5443 518 Size: 2042 Fragments arranged by size: 2042 1240 828 722 714 648 359 255 159 MspA1I CmG'CkG Cuts at: 1606 4383 5060 6208 6453 1606 Size: 2777 677 1148 245 2120 Fragments arranged by size: 2777 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5476 Fragments arranged by size: 5476 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6967 NheI G'CTAG_C Cuts at: 1052 2489 1052 Size: 1437 5530 NotI GC'GGCC_GC Cuts at: 4414 4414 Size: 6967 NspI r_CATG'y Cuts at: 1132 1229 2115 4325 5870 1132 Size: 97 886 2210 1545 2229 Fragments arranged by size: 2229 2210 1545 886 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6967 PciI A'CATG_T Cuts at: 5866 5866 Size: 6967 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6967 PfoI T'CCnGG_A Cuts at: 3635 3635 Size: 6967 PmeI GTTT'AAAC Cuts at: 4271 4271 Size: 6967 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5036 5068 6549 6581 5036 Size: 32 1481 32 5422 Fragments arranged by size: 5422 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6967 PsiI TTA'TAA Cuts at: 2184 3678 4537 2184 Size: 1494 859 4614 Fragments arranged by size: 4614 1494 859 PstI C_TGCA'G Cuts at: 830 4319 830 Size: 3489 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6967 SacI G_AGCT'C Cuts at: 3112 3896 3959 3112 Size: 784 63 6120 Fragments arranged by size: 6120 784 63 SalI G'TCGA_C Cuts at: 1936 4309 1936 Size: 2373 4594 SbfI CC_TGCA'GG Cuts at: 4319 4319 Size: 6967 ScaI AGT'ACT Cuts at: 5215 6927 5215 Size: 1712 5255 SfcI C'TryA_G Cuts at: 826 1045 4315 5450 6131 6322 826 Size: 219 3270 1135 681 191 1471 Fragments arranged by size: 3270 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6967 SmaI CCC'GGG Cuts at: 1872 4294 1872 Size: 2422 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2623 5071 5972 6234 820 Size: 197 926 680 2448 901 262 1553 Fragments arranged by size: 2448 1553 926 901 680 262 197 SnaBI TAC'GTA Cuts at: 493 4758 493 Size: 4265 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6967 SphI G_CATG'C Cuts at: 1132 4325 1132 Size: 3193 3774 SspI AAT'ATT Cuts at: 5 52 4891 5 Size: 47 4839 2081 Fragments arranged by size: 4839 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6967 StyI C'CwwG_G Cuts at: 513 1065 2004 3017 4406 513 Size: 552 939 1013 1389 3074 Fragments arranged by size: 3074 1389 1013 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4994 5011 5164 5349 5794 325 Size: 1255 3414 17 153 185 445 1498 Fragments arranged by size: 3414 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2135 3996 4201 Size: 274 80 33 51 1443 158 1861 205 Cuts at: 4201 5213 6925 96 Size: 1012 1712 138 Fragments arranged by size: 1861 1712 1443 1012 274 205 158 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2915 2947 6927 6959 2915 Size: 32 3980 32 2923 Fragments arranged by size: 3980 2923 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6967 XbaI T'CTAG_A Cuts at: 4303 4303 Size: 6967 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6967 XmaI C'CCGG_G Cuts at: 1870 4292 1870 Size: 2422 4545 XmnI GAAnn'nnTTC Cuts at: 3034 3325 5096 3034 Size: 291 1771 4905 Fragments arranged by size: 4905 1771 291 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4775 276 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI