(Linear) (Six-base) MAPSORT of: KIEE2081.seq Check: 8616 from: 1 to: 2262 KIEE2081.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AccI GT'mk_AC Cuts at: 0 808 2262 Size: 808 1454 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 219 689 2262 Size: 219 470 1573 Fragments arranged by size: 1573 470 219 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1738 2262 Size: 1738 524 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 28 2262 Size: 28 2234 ApoI r'AATT_y Cuts at: 0 319 708 1320 1968 2215 2262 Size: 319 389 612 648 247 47 Fragments arranged by size: 648 612 389 319 247 47 BanII G_rGCy'C Cuts at: 0 1107 1891 1954 2262 Size: 1107 784 63 308 Fragments arranged by size: 1107 784 308 63 BbsI GAAGACnn'nnnn_ Cuts at: 0 293 1340 2255 2262 Size: 293 1047 915 7 Fragments arranged by size: 1047 915 293 7 BciVI GTATCCnnnnn_n' Cuts at: 0 345 503 2262 Size: 345 158 1759 Fragments arranged by size: 1759 345 158 BclI T'GATC_A Cuts at: 0 1590 2007 2220 2262 Size: 1590 417 213 42 Fragments arranged by size: 1590 417 213 42 BglII A'GATC_T Cuts at: 0 982 2262 Size: 982 1280 Bme1580I G_kGCm'C Cuts at: 0 100 2262 Size: 100 2162 BmrI ACTGGGnnnn_n' Cuts at: 0 798 2262 Size: 798 1464 BmtI G_CTAG'C Cuts at: 0 488 2262 Size: 488 1774 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 603 2262 Size: 603 1659 BsaBI GATnn'nnATC Cuts at: 0 407 1041 2051 2262 Size: 407 634 1010 211 Fragments arranged by size: 1010 634 407 211 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1530 1560 2262 Size: 1530 30 702 Fragments arranged by size: 1530 702 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 269 383 582 585 1064 1878 1887 2262 Size: 269 114 199 3 479 814 9 375 Fragments arranged by size: 814 479 375 269 199 114 9 3 BseYI C'CCAG_C Cuts at: 0 944 2262 Size: 944 1318 BsiHKAI G_wGCw'C Cuts at: 0 1107 1891 1954 2262 Size: 1107 784 63 308 Fragments arranged by size: 1107 784 308 63 BsmBI CGTCTCn'nnnn_ Cuts at: 0 957 2262 Size: 957 1305 Bsp1286I G_dGCh'C Cuts at: 0 100 1107 1891 1954 2262 Size: 100 1007 784 63 308 Fragments arranged by size: 1007 784 308 100 63 BspHI T'CATG_A Cuts at: 0 1479 2223 2262 Size: 1479 744 39 Fragments arranged by size: 1479 744 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 66 2262 Size: 66 2196 BsrDI GCAATG_nn' Cuts at: 0 38 2262 Size: 38 2224 BsrGI T'GTAC_A Cuts at: 0 130 2196 2262 Size: 130 2066 66 Fragments arranged by size: 2066 130 66 BstAPI GCAn_nnn'nTGC Cuts at: 0 1095 2262 Size: 1095 1167 BstYI r'GATC_y Cuts at: 0 827 982 1036 2143 2262 Size: 827 155 54 1107 119 Fragments arranged by size: 1107 827 155 119 54 EaeI y'GGCC_r Cuts at: 0 335 2262 Size: 335 1927 EarI CTCTTCn'nnn_ Cuts at: 0 60 642 665 751 818 999 1713 1794 Size: 60 582 23 86 67 181 714 81 Cuts at: 1794 2262 Size: 468 Fragments arranged by size: 714 582 468 181 86 81 67 60 23 EcoICRI GAG'CTC Cuts at: 0 1105 1889 1952 2262 Size: 1105 784 63 310 Fragments arranged by size: 1105 784 310 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 219 689 2262 Size: 219 470 1573 Fragments arranged by size: 1573 470 219 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 416 448 616 648 852 884 1273 1305 Size: 416 32 168 32 204 32 389 32 Cuts at: 1305 2212 2244 2262 Size: 907 32 18 Fragments arranged by size: 907 416 389 204 168 32 32 32 32 32 18 MscI TGG'CCA Cuts at: 0 337 2262 Size: 337 1925 MslI CAynn'nnrTG Cuts at: 0 401 1229 1484 2198 2262 Size: 401 828 255 714 64 Fragments arranged by size: 828 714 401 255 64 NheI G'CTAG_C Cuts at: 0 484 2262 Size: 484 1778 NspI r_CATG'y Cuts at: 0 110 2262 Size: 110 2152 PfoI T'CCnGG_A Cuts at: 0 1630 2262 Size: 1630 632 PsiI TTA'TAA Cuts at: 0 179 1673 2262 Size: 179 1494 589 Fragments arranged by size: 1494 589 179 SacI G_AGCT'C Cuts at: 0 1107 1891 1954 2262 Size: 1107 784 63 308 Fragments arranged by size: 1107 784 308 63 SmlI C'TyrA_G Cuts at: 0 618 2262 Size: 618 1644 StyI C'CwwG_G Cuts at: 0 1012 2262 Size: 1012 1250 TatI w'GTAC_w Cuts at: 0 130 1991 2196 2262 Size: 130 1861 205 66 Fragments arranged by size: 1861 205 130 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 910 942 2262 Size: 910 32 1320 Fragments arranged by size: 1320 910 32 XmnI GAAnn'nnTTC Cuts at: 0 1029 1320 2262 Size: 1029 291 942 Fragments arranged by size: 1029 942 291 Enzymes that do cut: AccI AcuI AlfI AloI ApoI BanII BbsI BciVI BclI BglII Bme1580I BmrI BmtI BpuEI BsaBI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstYI EaeI EarI EcoICRI Eco57MI Hin4I MscI MslI NheI NspI PfoI PsiI SacI SmlI StyI TatI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI