(Circular) (Six-base) MAPSORT of: pFN21AB5778.seq Check: 8906 from: 1 to: 7003 pFN21AB5778 7003 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4813 278 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4208 4346 1937 Size: 2271 138 4594 Fragments arranged by size: 4594 2271 138 Acc65I G'GTAC_C Cuts at: 4324 4324 Size: 7003 AclI AA'CG_TT Cuts at: 1563 4009 4134 5130 5503 1563 Size: 2446 125 996 373 3063 Fragments arranged by size: 3063 2446 996 373 125 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2611 3066 3664 5066 6450 6718 1632 Size: 557 422 455 598 1402 1384 268 1917 Fragments arranged by size: 1917 1402 1384 598 557 455 422 268 AflII C'TTAA_G Cuts at: 820 1017 2182 820 Size: 197 1165 5641 Fragments arranged by size: 5641 1165 197 AflIII A'CryG_T Cuts at: 1203 5902 6879 1203 Size: 4699 977 1327 Fragments arranged by size: 4699 1327 977 AgeI A'CCGG_T Cuts at: 5834 5834 Size: 7003 AhdI GACnn_n'nnGTC Cuts at: 5732 5732 Size: 7003 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7003 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7003 AlwNI CAG_nnn'CTG Cuts at: 2786 3674 4746 6318 2786 Size: 888 1072 1572 3471 Fragments arranged by size: 3471 1572 1072 888 ApaLI G'TGCA_C Cuts at: 5060 6216 5060 Size: 1156 5847 ApoI r'AATT_y Cuts at: 1493 2539 2934 3572 4057 4084 4312 4538 4690 Size: 1046 395 638 485 27 228 226 152 Cuts at: 4690 6799 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1046 638 485 395 228 226 152 27 AseI AT'TA_AT Cuts at: 160 1032 5557 160 Size: 872 4525 1606 Fragments arranged by size: 4525 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 AvaI C'yCGr_G Cuts at: 1870 1943 4328 1870 Size: 73 2385 4545 Fragments arranged by size: 4545 2385 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2729 2762 2729 Size: 33 6970 BamHI G'GATC_C Cuts at: 4333 4700 4333 Size: 367 6636 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3203 3964 4324 5779 618 Size: 325 217 627 1416 761 360 1455 1842 Fragments arranged by size: 1842 1455 1416 761 627 360 325 217 BanII G_rGCy'C Cuts at: 1391 4322 1391 Size: 2931 4072 BbeI G_GCGC'C Cuts at: 3968 3968 Size: 7003 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 2617 3495 3670 3799 4041 4224 Size: 971 116 602 878 175 129 242 183 Cuts at: 4224 928 Size: 3707 Fragments arranged by size: 3707 971 878 602 242 183 175 129 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5194 5228 1903 Size: 34 3257 34 3678 Fragments arranged by size: 3678 3257 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3982 4895 6105 3982 Size: 913 1210 4880 Fragments arranged by size: 4880 1210 913 BclI T'GATC_A Cuts at: 1548 1548 Size: 7003 BfrBI ATG'CAT Cuts at: 3778 3778 Size: 7003 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5614 136 Size: 107 122 71 631 605 3942 1525 Fragments arranged by size: 3942 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4158 6998 4158 Size: 2840 4163 BlpI GC'TnA_GC Cuts at: 4420 4420 Size: 7003 Bme1580I G_kGCm'C Cuts at: 1790 2076 5064 6220 1790 Size: 286 2988 1156 2573 Fragments arranged by size: 2988 2573 1156 286 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7003 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2614 3700 3806 Size: 416 53 83 399 24 1197 1086 106 Cuts at: 3806 4734 5692 442 Size: 928 958 1753 Fragments arranged by size: 1753 1197 1086 958 928 416 399 106 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3735 4236 1056 Size: 2679 501 3823 Fragments arranged by size: 3823 2679 501 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4014 5663 1239 Size: 2775 1649 2579 Fragments arranged by size: 2775 2579 1649 Bpu10I CC'TnA_GC Cuts at: 2951 2951 Size: 7003 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5128 5993 6291 5128 Size: 865 298 5840 Fragments arranged by size: 5840 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2658 4025 5666 882 Size: 617 1159 1367 1641 2219 Fragments arranged by size: 2219 1641 1367 1159 617 BsaAI yAC'GTr Cuts at: 493 1204 2365 3907 4794 493 Size: 711 1161 1542 887 2702 Fragments arranged by size: 2702 1542 1161 887 711 BsaBI GATnn'nnATC Cuts at: 6997 6997 Size: 7003 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3965 4810 5192 5827 Size: 53 83 186 936 2432 845 382 635 Cuts at: 5827 275 Size: 1451 Fragments arranged by size: 2432 1451 936 845 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3355 3422 5436 5834 6108 6255 6666 3355 Size: 67 2014 398 274 147 411 3692 Fragments arranged by size: 3692 2014 411 398 274 147 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3891 3921 1178 Size: 30 165 30 2488 30 4260 Fragments arranged by size: 4260 2488 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3588 1186 Size: 2402 4601 BseYI C'CCAG_C Cuts at: 2038 3060 6206 6702 2038 Size: 1022 3146 496 2339 Fragments arranged by size: 3146 2339 1022 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3169 1163 Size: 573 1433 4997 Fragments arranged by size: 4997 1433 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4453 5214 6242 1540 Size: 275 186 2452 761 1028 2301 Fragments arranged by size: 2452 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5064 5149 6220 5064 Size: 85 1071 5847 Fragments arranged by size: 5847 1071 85 BsmI GAATG_Cn' Cuts at: 2380 3282 4514 4607 2380 Size: 902 1232 93 4776 Fragments arranged by size: 4776 1232 902 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 4322 5064 5149 6220 1391 Size: 399 286 2246 742 85 1071 2174 Fragments arranged by size: 2246 2174 1071 742 399 286 85 BspEI T'CCGG_A Cuts at: 3355 6666 3355 Size: 3311 3692 BspHI T'CATG_A Cuts at: 2200 4068 4892 6622 2200 Size: 1868 824 1730 2581 Fragments arranged by size: 2581 1868 1730 824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4358 844 Size: 3514 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4890 1394 Size: 429 3067 3507 Fragments arranged by size: 3507 3067 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2378 3139 4206 5498 5672 65 Size: 1183 1130 761 1067 1292 174 1396 Fragments arranged by size: 1396 1292 1183 1130 1067 761 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5647 5834 1700 Size: 110 3837 187 2869 Fragments arranged by size: 3837 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3999 96 Size: 3903 3100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7003 BssSI C'ACGA_G Cuts at: 5063 6075 5063 Size: 1012 5991 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7003 BstBI TT'CG_AA Cuts at: 5807 5807 Size: 7003 BstEII G'GTnAC_C Cuts at: 1184 2475 3755 1184 Size: 1291 1280 4432 Fragments arranged by size: 4432 1291 1280 BstXI CCAn_nnnn'nTGG Cuts at: 3544 4171 3544 Size: 627 6376 BstYI r'GATC_y Cuts at: 1972 4158 4333 4700 5085 5102 6554 6640 6652 Size: 2186 175 367 385 17 1452 86 12 Cuts at: 6652 6998 1972 Size: 346 1977 Fragments arranged by size: 2186 1977 1452 385 367 346 175 86 17 12 BstZ17I GTA'TAC Cuts at: 4209 4209 Size: 7003 BtgI C'CryG_G Cuts at: 513 1065 2015 2076 3960 4222 513 Size: 552 950 61 1884 262 3294 Fragments arranged by size: 3294 1884 950 552 262 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6264 Fragments arranged by size: 6264 683 56 BtsI GCAGTG_nn' Cuts at: 2738 2865 2951 4519 5313 5333 2738 Size: 127 86 1568 794 20 4408 Fragments arranged by size: 4408 1568 794 127 86 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7003 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3685 3720 529 Size: 35 3121 35 3812 Fragments arranged by size: 3812 3121 35 35 DraI TTT'AAA Cuts at: 2415 4090 4307 2415 Size: 1675 217 5111 Fragments arranged by size: 5111 1675 217 DraIII CAC_nnn'GTG Cuts at: 2463 2463 Size: 7003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2699 6010 809 Size: 507 1383 3311 1802 Fragments arranged by size: 3311 1802 1383 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4450 5339 6968 Size: 54 1425 50 275 18 2620 889 1629 Cuts at: 6968 8 Size: 43 Fragments arranged by size: 2620 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4450 1537 Size: 275 2638 4090 Fragments arranged by size: 4090 2638 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2433 2610 4933 1353 Size: 1080 177 2323 3423 Fragments arranged by size: 3423 2323 1080 177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5590 5964 6110 387 Size: 1064 138 4001 374 146 1280 Fragments arranged by size: 4001 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2611 3066 3664 4014 5066 5663 Size: 393 557 422 455 598 350 1052 597 Cuts at: 5663 6450 6718 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1052 787 598 597 557 455 422 393 350 268 EcoRI G'AATT_C Cuts at: 4312 4690 4312 Size: 378 6625 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2723 2955 4058 2723 Size: 232 1103 5668 Fragments arranged by size: 5668 1103 232 FspI TGC'GCA Cuts at: 5509 5509 Size: 7003 HaeII r_GCGC'y Cuts at: 1678 3968 6150 1678 Size: 2290 2182 2531 Fragments arranged by size: 2531 2290 2182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2182 2214 2292 2324 2878 Size: 32 135 32 610 32 78 32 554 Cuts at: 2878 2910 3214 3246 3580 3612 3756 3788 5644 Size: 32 304 32 334 32 144 32 1856 Cuts at: 5644 5676 5718 5750 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1856 610 554 334 304 144 135 78 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4347 4593 669 Size: 988 281 2409 246 3079 Fragments arranged by size: 3079 2409 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7003 HpaI GTT'AAC Cuts at: 4593 4593 Size: 7003 KasI G'GCGC_C Cuts at: 3964 3964 Size: 7003 KpnI G_GTAC'C Cuts at: 4328 4328 Size: 7003 MfeI C'AATT_G Cuts at: 4602 4602 Size: 7003 MluI A'CGCG_T Cuts at: 6879 6879 Size: 7003 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2819 3046 3331 4162 6117 6301 1305 Size: 1514 227 285 831 1955 184 2007 Fragments arranged by size: 2007 1955 1514 831 285 227 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5181 Fragments arranged by size: 5181 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 3044 4961 5320 5479 518 Size: 648 903 975 1917 359 159 2042 Fragments arranged by size: 2042 1917 975 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2114 2428 3412 4419 5096 6244 6489 1606 Size: 508 314 984 1007 677 1148 245 2120 Fragments arranged by size: 2120 1148 1007 984 677 508 314 245 NarI GG'CG_CC Cuts at: 3965 3965 Size: 7003 NcoI C'CATG_G Cuts at: 513 1065 4222 513 Size: 552 3157 3294 Fragments arranged by size: 3294 3157 552 NdeI CA'TA_TG Cuts at: 387 2398 2500 2677 3895 387 Size: 2011 102 177 1218 3495 Fragments arranged by size: 3495 2011 1218 177 102 NheI G'CTAG_C Cuts at: 1052 3731 4232 1052 Size: 2679 501 3823 Fragments arranged by size: 3823 2679 501 NotI GC'GGCC_GC Cuts at: 4450 4450 Size: 7003 NsiI A_TGCA'T Cuts at: 3780 3780 Size: 7003 NspI r_CATG'y Cuts at: 1132 1229 3336 3782 4361 5906 1132 Size: 97 2107 446 579 1545 2229 Fragments arranged by size: 2229 2107 1545 579 446 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7003 PciI A'CATG_T Cuts at: 5902 5902 Size: 7003 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7003 PfoI T'CCnGG_A Cuts at: 3483 3483 Size: 7003 PmeI GTTT'AAAC Cuts at: 4307 4307 Size: 7003 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5072 5104 6585 6617 5072 Size: 32 1481 32 5458 Fragments arranged by size: 5458 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7003 PsiI TTA'TAA Cuts at: 3300 4573 3300 Size: 1273 5730 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2526 2558 2526 Size: 32 6971 PstI C_TGCA'G Cuts at: 830 2745 3519 4355 830 Size: 1915 774 836 3478 Fragments arranged by size: 3478 1915 836 774 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7003 PvuII CAG'CTG Cuts at: 2114 2428 3412 2114 Size: 314 984 5705 Fragments arranged by size: 5705 984 314 SalI G'TCGA_C Cuts at: 1936 4345 1936 Size: 2409 4594 SbfI CC_TGCA'GG Cuts at: 4355 4355 Size: 7003 ScaI AGT'ACT Cuts at: 3052 3371 3986 5251 6963 3052 Size: 319 615 1265 1712 3092 Fragments arranged by size: 3092 1712 1265 615 319 SfcI C'TryA_G Cuts at: 826 1045 2109 2741 3515 4351 5486 6167 6358 Size: 219 1064 632 774 836 1135 681 191 Cuts at: 6358 826 Size: 1471 Fragments arranged by size: 1471 1135 1064 836 774 681 632 219 191 SfoI GGC'GCC Cuts at: 3966 3966 Size: 7003 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7003 SmaI CCC'GGG Cuts at: 1872 4330 1872 Size: 2458 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2182 5107 6008 6270 820 Size: 197 926 239 2925 901 262 1553 Fragments arranged by size: 2925 1553 926 901 262 239 197 SnaBI TAC'GTA Cuts at: 493 3907 4794 493 Size: 3414 887 2702 Fragments arranged by size: 3414 2702 887 SpeI A'CTAG_T Cuts at: 152 152 Size: 7003 SphI G_CATG'C Cuts at: 1132 3782 4361 1132 Size: 2650 579 3774 Fragments arranged by size: 3774 2650 579 SspI AAT'ATT Cuts at: 5 52 4927 5 Size: 47 4875 2081 Fragments arranged by size: 4875 2081 47 StuI AGG'CCT Cuts at: 1517 3079 1517 Size: 1562 5441 StyI C'CwwG_G Cuts at: 513 1065 3760 4222 4442 513 Size: 552 2695 462 220 3074 Fragments arranged by size: 3074 2695 552 462 220 SwaI ATTT'AAAT Cuts at: 2415 2415 Size: 7003 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 2962 5030 5047 5200 5385 5830 Size: 1255 450 932 2068 17 153 185 445 Cuts at: 5830 325 Size: 1498 Fragments arranged by size: 2068 1498 1255 932 450 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2282 3050 3369 Size: 274 80 33 51 1443 305 768 319 Cuts at: 3369 3984 3999 5249 6961 96 Size: 615 15 1250 1712 138 Fragments arranged by size: 1712 1443 1250 768 615 319 305 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3924 3945 3956 3977 6963 6995 3924 Size: 21 11 21 2986 32 3932 Fragments arranged by size: 3932 2986 32 21 21 11 Tth111I GACn'n_nGTC Cuts at: 1316 3674 1316 Size: 2358 4645 XbaI T'CTAG_A Cuts at: 4339 4339 Size: 7003 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7003 XmaI C'CCGG_G Cuts at: 1870 4328 1870 Size: 2458 4545 XmnI GAAnn'nnTTC Cuts at: 5132 5132 Size: 7003 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4811 276 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BplI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI XcmI