(Linear) (Six-base) MAPSORT of: KIBB5778.seq Check: 6966 from: 1 to: 2298 KIBB5778.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AccI GT'mk_AC Cuts at: 0 2203 2298 Size: 2203 95 AclI AA'CG_TT Cuts at: 0 2004 2129 2298 Size: 2004 125 169 Fragments arranged by size: 2004 169 125 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 606 1061 1659 2298 Size: 184 422 455 598 639 Fragments arranged by size: 639 598 455 422 184 AflII C'TTAA_G Cuts at: 0 177 2298 Size: 177 2121 AlwNI CAG_nnn'CTG Cuts at: 0 781 1669 2298 Size: 781 888 629 Fragments arranged by size: 888 781 629 ApoI r'AATT_y Cuts at: 0 534 929 1567 2052 2079 2298 Size: 534 395 638 485 27 219 Fragments arranged by size: 638 534 485 395 219 27 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 724 757 2298 Size: 724 33 1541 Fragments arranged by size: 1541 724 33 BanI G'GyrC_C Cuts at: 0 1198 1959 2298 Size: 1198 761 339 Fragments arranged by size: 1198 761 339 BbeI G_GCGC'C Cuts at: 0 1963 2298 Size: 1963 335 BbsI GAAGACnn'nnnn_ Cuts at: 0 10 612 1490 1665 1794 2036 2219 2298 Size: 10 602 878 175 129 242 183 79 Fragments arranged by size: 878 602 242 183 175 129 79 10 BciVI GTATCCnnnnn_n' Cuts at: 0 1977 2298 Size: 1977 321 BfrBI ATG'CAT Cuts at: 0 1773 2298 Size: 1773 525 BglII A'GATC_T Cuts at: 0 2153 2298 Size: 2153 145 Bme1580I G_kGCm'C Cuts at: 0 71 2298 Size: 71 2227 BmrI ACTGGGnnnn_n' Cuts at: 0 609 1695 1801 2298 Size: 609 1086 106 497 Fragments arranged by size: 1086 609 497 106 BmtI G_CTAG'C Cuts at: 0 1730 2231 2298 Size: 1730 501 67 Fragments arranged by size: 1730 501 67 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2009 2298 Size: 2009 289 Bpu10I CC'TnA_GC Cuts at: 0 946 2298 Size: 946 1352 BsaI GGTCTCn'nnnn_ Cuts at: 0 653 2020 2298 Size: 653 1367 278 Fragments arranged by size: 1367 653 278 BsaAI yAC'GTr Cuts at: 0 360 1902 2298 Size: 360 1542 396 Fragments arranged by size: 1542 396 360 BsaHI Gr'CG_yC Cuts at: 0 1960 2298 Size: 1960 338 BsaWI w'CCGG_w Cuts at: 0 1350 1417 2298 Size: 1350 67 881 Fragments arranged by size: 1350 881 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1886 1916 2298 Size: 1886 30 382 Fragments arranged by size: 1886 382 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1583 2298 Size: 1583 715 BseYI C'CCAG_C Cuts at: 0 33 1055 2298 Size: 33 1022 1243 Fragments arranged by size: 1243 1022 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1164 2298 Size: 1164 1134 BsmI GAATG_Cn' Cuts at: 0 375 1277 2298 Size: 375 902 1021 Fragments arranged by size: 1021 902 375 Bsp1286I G_dGCh'C Cuts at: 0 71 2298 Size: 71 2227 BspEI T'CCGG_A Cuts at: 0 1350 2298 Size: 1350 948 BspHI T'CATG_A Cuts at: 0 195 2063 2298 Size: 195 1868 235 Fragments arranged by size: 1868 235 195 BsrDI GCAATG_nn' Cuts at: 0 373 1134 2201 2298 Size: 373 761 1067 97 Fragments arranged by size: 1067 761 373 97 BsrGI T'GTAC_A Cuts at: 0 1994 2298 Size: 1994 304 BstEII G'GTnAC_C Cuts at: 0 470 1750 2298 Size: 470 1280 548 Fragments arranged by size: 1280 548 470 BstXI CCAn_nnnn'nTGG Cuts at: 0 1539 2166 2298 Size: 1539 627 132 Fragments arranged by size: 1539 627 132 BstYI r'GATC_y Cuts at: 0 2153 2298 Size: 2153 145 BstZ17I GTA'TAC Cuts at: 0 2204 2298 Size: 2204 94 BtgI C'CryG_G Cuts at: 0 10 71 1955 2217 2298 Size: 10 61 1884 262 81 Fragments arranged by size: 1884 262 81 61 10 BtsI GCAGTG_nn' Cuts at: 0 733 860 946 2298 Size: 733 127 86 1352 Fragments arranged by size: 1352 733 127 86 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1680 1715 2298 Size: 1680 35 583 Fragments arranged by size: 1680 583 35 DraI TTT'AAA Cuts at: 0 410 2085 2298 Size: 410 1675 213 Fragments arranged by size: 1675 410 213 DraIII CAC_nnn'GTG Cuts at: 0 458 2298 Size: 458 1840 DrdI GACnn_nn'nnGTC Cuts at: 0 694 2298 Size: 694 1604 EarI CTCTTCn'nnn_ Cuts at: 0 428 605 2298 Size: 428 177 1693 Fragments arranged by size: 1693 428 177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 606 1061 1659 2009 2298 Size: 184 422 455 598 350 289 Fragments arranged by size: 598 455 422 350 289 184 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 718 950 2053 2298 Size: 718 232 1103 245 Fragments arranged by size: 1103 718 245 232 HaeII r_GCGC'y Cuts at: 0 1963 2298 Size: 1963 335 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 177 209 287 319 873 905 1209 1241 Size: 177 32 78 32 554 32 304 32 Cuts at: 1241 1575 1607 1751 1783 2298 Size: 334 32 144 32 515 Fragments arranged by size: 554 515 334 304 177 144 78 32 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1959 2298 Size: 1959 339 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 814 1041 1326 2157 2298 Size: 814 227 285 831 141 Fragments arranged by size: 831 814 285 227 141 MslI CAynn'nnrTG Cuts at: 0 64 1039 2298 Size: 64 975 1259 Fragments arranged by size: 1259 975 64 MspA1I CmG'CkG Cuts at: 0 109 423 1407 2298 Size: 109 314 984 891 Fragments arranged by size: 984 891 314 109 NarI GG'CG_CC Cuts at: 0 1960 2298 Size: 1960 338 NcoI C'CATG_G Cuts at: 0 2217 2298 Size: 2217 81 NdeI CA'TA_TG Cuts at: 0 393 495 672 1890 2298 Size: 393 102 177 1218 408 Fragments arranged by size: 1218 408 393 177 102 NheI G'CTAG_C Cuts at: 0 1726 2227 2298 Size: 1726 501 71 Fragments arranged by size: 1726 501 71 NsiI A_TGCA'T Cuts at: 0 1775 2298 Size: 1775 523 NspI r_CATG'y Cuts at: 0 1331 1777 2298 Size: 1331 446 521 Fragments arranged by size: 1331 521 446 PfoI T'CCnGG_A Cuts at: 0 1478 2298 Size: 1478 820 PsiI TTA'TAA Cuts at: 0 1295 2298 Size: 1295 1003 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 521 553 2298 Size: 521 32 1745 Fragments arranged by size: 1745 521 32 PstI C_TGCA'G Cuts at: 0 740 1514 2298 Size: 740 774 784 Fragments arranged by size: 784 774 740 PvuII CAG'CTG Cuts at: 0 109 423 1407 2298 Size: 109 314 984 891 Fragments arranged by size: 984 891 314 109 ScaI AGT'ACT Cuts at: 0 1047 1366 1981 2298 Size: 1047 319 615 317 Fragments arranged by size: 1047 615 319 317 SfcI C'TryA_G Cuts at: 0 104 736 1510 2298 Size: 104 632 774 788 Fragments arranged by size: 788 774 632 104 SfoI GGC'GCC Cuts at: 0 1961 2298 Size: 1961 337 SmlI C'TyrA_G Cuts at: 0 177 2298 Size: 177 2121 SnaBI TAC'GTA Cuts at: 0 1902 2298 Size: 1902 396 SphI G_CATG'C Cuts at: 0 1777 2298 Size: 1777 521 StuI AGG'CCT Cuts at: 0 1074 2298 Size: 1074 1224 StyI C'CwwG_G Cuts at: 0 1755 2217 2298 Size: 1755 462 81 Fragments arranged by size: 1755 462 81 SwaI ATTT'AAAT Cuts at: 0 410 2298 Size: 410 1888 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 25 957 2298 Size: 25 932 1341 Fragments arranged by size: 1341 932 25 TatI w'GTAC_w Cuts at: 0 277 1045 1364 1979 1994 2298 Size: 277 768 319 615 15 304 Fragments arranged by size: 768 615 319 304 277 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1919 1940 1951 1972 2298 Size: 1919 21 11 21 326 Fragments arranged by size: 1919 326 21 21 11 Tth111I GACn'n_nGTC Cuts at: 0 1669 2298 Size: 1669 629 Enzymes that do cut: AccI AclI AcuI AflII AlwNI ApoI BaeI BanI BbeI BbsI BciVI BfrBI BglII Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspEI BspHI BsrDI BsrGI BstEII BstXI BstYI BstZ17I BtgI BtsI CspCI DraI DraIII DrdI EarI Eco57MI FalI HaeII Hin4I KasI MmeI MslI MspA1I NarI NcoI NdeI NheI NsiI NspI PfoI PsiI PsrI PstI PvuII ScaI SfcI SfoI SmlI SnaBI SphI StuI StyI SwaI TaqII TatI TstI Tth111I Enzymes that do not cut: AarI AatII Acc65I AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BanII BbvCI BcgI BclI BglI BlpI BmgBI BplI BpuEI BsaBI BsiEI BsiHKAI BsiWI BsmBI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI Bsu36I BtgZI ClaI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NgoMIV NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SpeI SrfI SspI XbaI XcmI XhoI XmaI XmnI ZraI