Restriction map for
pFN21AB6594
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BcnI BfrI BglII BlnI BpmI
BsaBI BsaI BseAI BsgI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI HinP1I HindIII
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1176 GACGTC GACGT^C
AccI 322 GTCGAC GT^[AC][GT]AC
AcsI 610 AAATTT [GA]^AATT[CT]
AcyI 1176 GACGTC G[GA]^CG[CT]C
ApoI 610 AAATTT [GA]^AATT[CT]
AspHI 976 GAGCAC G[AT]GC[AT]^C
BanI 952 GGTGCC G^G[CT][GA]CC
BbsI 722 GAAGAC GAAGAC(2/6)
BciVI 512 GGATAC GTATCC(6/5)
BglI 364 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 174 GCTCAGC GC^T[ACGT]AGC
BmyI 976 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 174 GCTCAGC GC^T[ACGT]AGC
BpuAI 722 GAAGAC GAAGAC(2/6)
BsaAI 518 TACGTG [CT]AC^GT[GA]
BsaHI 1176 GACGTC G[GA]^CG[CT]C
BsaWI 1054 ACCGGA [AT]^CCGG[AT]
BseRI 1141 GAGGAG GAGGAG(10/8)
BsiHKAI 976 GAGCAC G[AT]GC[AT]^C
BsmBI 1174 GAGACG CGTCTC(1/5)
Bsp1286I 976 GAGCAC G[AGT]GC[ACT]^C
BspMI 1161 GCAGGT ACCTGC(4/8)
BstYI 414 AGATCC [GA]^GATC[CT]
CelII 174 GCTCAGC GC^T[ACGT]AGC
DraII 227 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 227 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 997 CCCGC CCCGC(4/6)
HapII 1055 CCGG C^CGG
Hin1I 1176 GACGTC G[GA]^CG[CT]C
HpaI 960 GTTAAC GTT^AAC
HpaII 1055 CCGG C^CGG
MboII 722 GAAGA GAAGA(8/7)
MflI 414 AGATCC [GA]^GATC[CT]
MspI 1055 CCGG C^CGG
NspI 1064 ACATGC [GA]CATG^[CT]
PleI 1032 GAGTC GAGTC(4/5)
PpuMI 227 AGGACCT [GA]G^G[AT]CC[CT]
PstI 338 CTGCAG CTGCA^G
PvuII 788 CAGCTG CAG^CTG
SalI 322 GTCGAC G^TCGAC
XcmI 71 CCAAGCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 414 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 998 CCGC CCGC(-3/-1)
1129 GCGG
AlwI 415 GATCC GGATC(4/5)
168 GGATC
AvaII 84 GGTCC G^G[AT]CC
228 GGACC
BclI 845 TGATCA T^GATCA
1007 TGATCA
BsiYI 93 CCACCCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
225 CCAGGACCTGG
BslI 93 CCACCCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
225 CCAGGACCTGG
BsmFI 85 GTCCC GGGAC(10/14)
113 GTCCC
FbaI 845 TGATCA T^GATCA
1007 TGATCA
MaeIII 987 GTCAC ^GT[ACGT]AC
1041 GTCAC
MslI 598 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
946 CACCCTGGTG
MspA1I 788 CAGCTG C[AC]G^C[GT]G
1127 CAGCGG
NdeI 251 CATATG CA^TATG
1114 CATATG
PflMI 93 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
225 CCAGGACCTGG
SexAI 230 ACCTGGT A^CC[AT]GGT
896 ACCAGGT
TaqI 323 TCGA T^CGA
1172 TCGA
TfiI 523 GATTC G^A[AT]TC
565 GAATC
Tsp45I 987 GTCAC ^GT[GC]AC
1041 GTCAC
TspRI 375 CAGTG CA[GC]TG[ACGT][ACGT]^
528 CAGTG
TthHB8I 323 TCGA T^CGA
1172 TCGA
Van91I 93 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
225 CCAGGACCTGG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AfaI AluI AlwNI BbvI BfaI
BsaJI BsmAI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI MaeI MaeII MboI MnlI
MseI MvaI MwoI NcoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI Tru9I
TseI Tsp509I