(Circular) (Six-base) MAPSORT of: pFN21AB6594.seq Check: 2126 from: 1 to: 5923 pFN21AB6594 5923 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3157 3157 Size: 5923 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3185 3733 278 Size: 53 83 186 936 1649 548 2468 Fragments arranged by size: 2468 1649 936 548 186 83 53 AccI GT'mk_AC Cuts at: 1937 2328 3266 1937 Size: 391 938 4594 Fragments arranged by size: 4594 938 391 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2393 3986 5370 5638 1632 Size: 761 1593 1384 268 1917 Fragments arranged by size: 1917 1593 1384 761 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 2955 1166 Size: 1789 4134 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2973 1184 Size: 1789 4134 AlwNI CAG_nnn'CTG Cuts at: 2068 2086 2236 3666 5238 2068 Size: 18 150 1430 1572 2753 Fragments arranged by size: 2753 1572 1430 150 18 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2615 3232 3458 3610 5719 1493 Size: 1122 617 226 152 2109 1697 Fragments arranged by size: 2109 1697 1122 617 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 3248 1870 Size: 73 1305 4545 Fragments arranged by size: 4545 1305 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2957 3244 4699 618 Size: 325 217 627 1170 287 1455 1842 Fragments arranged by size: 1842 1455 1170 627 325 287 217 BanII G_rGCy'C Cuts at: 1391 3242 1391 Size: 1851 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2734 928 Size: 971 835 4117 Fragments arranged by size: 4117 971 835 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2511 3815 5025 2511 Size: 1304 1210 3409 Fragments arranged by size: 3409 1304 1210 BclI T'GATC_A Cuts at: 1548 2850 3012 1548 Size: 1302 162 4459 Fragments arranged by size: 4459 1302 162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2375 4534 136 Size: 107 122 71 631 605 703 2159 1525 Fragments arranged by size: 2159 1525 703 631 605 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 2180 3340 2180 Size: 1160 4763 Bme1580I G_kGCm'C Cuts at: 1790 3984 5140 1790 Size: 2194 1156 2573 Fragments arranged by size: 2573 2194 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2056 2109 3654 Size: 416 53 83 399 24 639 53 1545 Cuts at: 3654 4612 442 Size: 958 1753 Fragments arranged by size: 1753 1545 958 639 416 399 83 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4583 1239 Size: 3344 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2525 3714 493 Size: 711 1321 1189 2702 Fragments arranged by size: 2702 1321 1189 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3182 3730 4112 4747 Size: 53 83 186 936 1649 548 382 635 Cuts at: 4747 275 Size: 1451 Fragments arranged by size: 1649 1451 936 635 548 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3059 4356 4754 5028 5175 5586 3059 Size: 1297 398 274 147 411 3396 Fragments arranged by size: 3396 1297 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5698 Fragments arranged by size: 5698 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3161 1186 Size: 1975 3948 BseYI C'CCAG_C Cuts at: 2092 2120 3027 5126 5622 2092 Size: 28 907 2099 496 2393 Fragments arranged by size: 2393 2099 907 496 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5350 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2985 3984 4069 5140 2985 Size: 999 85 1071 3768 Fragments arranged by size: 3768 1071 999 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 BsmBI CGTCTCn'nnnn_ Cuts at: 3173 3173 Size: 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2985 3242 3984 4069 5140 1391 Size: 399 1195 257 742 85 1071 2174 Fragments arranged by size: 2174 1195 1071 742 399 257 85 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3157 3278 844 Size: 2313 121 3489 Fragments arranged by size: 3489 2313 121 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2419 3253 3620 4005 4022 5474 5560 5572 Size: 447 834 367 385 17 1452 86 12 Cuts at: 5572 5918 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 834 447 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2103 2457 2732 513 Size: 552 939 99 354 275 3704 Fragments arranged by size: 3704 939 552 354 275 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 3439 4233 4253 3439 Size: 794 20 5109 Fragments arranged by size: 5109 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3370 4259 5888 Size: 54 1425 50 275 18 1540 889 1629 Cuts at: 5888 8 Size: 43 Fragments arranged by size: 1629 1540 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2393 3986 4583 5370 5638 1239 Size: 393 761 1593 597 787 268 1524 Fragments arranged by size: 1593 1524 787 761 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2233 2233 Size: 5923 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 5070 1678 Size: 3392 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2052 2914 2946 3004 Size: 32 135 32 448 32 862 32 58 Cuts at: 3004 3025 3036 3057 4564 4596 4638 4670 1373 Size: 21 11 21 1507 32 42 32 2626 Fragments arranged by size: 2626 1507 862 448 135 58 42 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2252 2329 2967 3161 3267 3513 Size: 988 281 314 77 638 194 106 246 Cuts at: 3513 669 Size: 3079 Fragments arranged by size: 3079 988 638 314 281 246 194 106 77 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 2967 3513 2967 Size: 546 5377 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2607 2955 3881 4240 4399 518 Size: 648 1441 348 926 359 159 2042 Fragments arranged by size: 2042 1441 926 648 359 348 159 MspA1I CmG'CkG Cuts at: 1606 2795 3134 3339 4016 5164 5409 1606 Size: 1189 339 205 677 1148 245 2120 Fragments arranged by size: 2120 1189 1148 677 339 245 205 NcoI C'CATG_G Cuts at: 513 1065 2004 2103 2457 2732 513 Size: 552 939 99 354 275 3704 Fragments arranged by size: 3704 939 552 354 275 99 NdeI CA'TA_TG Cuts at: 387 2257 3120 387 Size: 1870 863 3190 Fragments arranged by size: 3190 1870 863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3073 3281 4826 1132 Size: 97 1844 208 1545 2229 Fragments arranged by size: 2229 1844 1545 208 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2104 2236 1108 Size: 996 132 4795 Fragments arranged by size: 4795 996 132 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2233 2233 Size: 5923 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PstI C_TGCA'G Cuts at: 830 2347 3275 830 Size: 1517 928 3478 Fragments arranged by size: 3478 1517 928 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2795 2795 Size: 5923 SalI G'TCGA_C Cuts at: 1936 2327 3265 1936 Size: 391 938 4594 Fragments arranged by size: 4594 938 391 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SexAI A'CCwGG_T Cuts at: 2235 2901 2235 Size: 666 5257 SfcI C'TryA_G Cuts at: 826 1045 2343 2790 2945 2996 3271 4406 5087 Size: 219 1298 447 155 51 275 1135 681 Cuts at: 5087 5278 826 Size: 191 1471 Fragments arranged by size: 1471 1298 1135 681 447 275 219 191 155 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4027 4928 5190 820 Size: 197 926 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 2103 2457 2732 3362 513 Size: 552 939 99 354 275 630 3074 Fragments arranged by size: 3074 939 630 552 354 275 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3950 3967 4120 4305 4750 325 Size: 1255 2370 17 153 185 445 1498 Fragments arranged by size: 2370 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4169 5881 96 Size: 274 80 33 51 1443 2192 1712 138 Fragments arranged by size: 2192 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2509 2541 5883 5915 2509 Size: 32 3342 32 2517 Fragments arranged by size: 3342 2517 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2083 2083 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3183 3731 276 Size: 53 83 186 936 1649 548 2468 Fragments arranged by size: 2468 1649 936 548 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI