Restriction map for
pFN21AB6594
(Linear) (Six-base) MAPSORT of: KIBB6594.seq Check: 84 from: 1 to: 1218
KIBB6594.orf
With 182 enzymes: SgfI *
January 29, 2014 11:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1152 1218
Size: 1152 66
AatII G_ACGT'C
Cuts at: 0 1180 1218
Size: 1180 38
AccI GT'mk_AC
Cuts at: 0 323 1218
Size: 323 895
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1218
Size: 388 830
AleI CACnn'nnGTG
Cuts at: 0 950 1218
Size: 950 268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 968 1218
Size: 968 250
AlwNI CAG_nnn'CTG
Cuts at: 0 63 81 231 1218
Size: 63 18 150 987
Fragments arranged by size:
987 150 63 18
ApoI r'AATT_y
Cuts at: 0 610 1218
Size: 610 608
BanI G'GyrC_C
Cuts at: 0 952 1218
Size: 952 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 729 1218
Size: 729 489
BciVI GTATCCnnnnn_n'
Cuts at: 0 506 1218
Size: 506 712
BclI T'GATC_A
Cuts at: 0 845 1007 1218
Size: 845 162 211
Fragments arranged by size:
845 211 162
BglI GCCn_nnn'nGGC
Cuts at: 0 370 1218
Size: 370 848
BlpI GC'TnA_GC
Cuts at: 0 175 1218
Size: 175 1043
BmrI ACTGGGnnnn_n'
Cuts at: 0 51 104 1218
Size: 51 53 1114
Fragments arranged by size:
1114 53 51
BsaAI yAC'GTr
Cuts at: 0 520 1218
Size: 520 698
BsaHI Gr'CG_yC
Cuts at: 0 1177 1218
Size: 1177 41
BsaWI w'CCGG_w
Cuts at: 0 1054 1218
Size: 1054 164
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1156 1218
Size: 1156 62
BseYI C'CCAG_C
Cuts at: 0 87 115 1022 1218
Size: 87 28 907 196
Fragments arranged by size:
907 196 87 28
BsiHKAI G_wGCw'C
Cuts at: 0 980 1218
Size: 980 238
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1168 1218
Size: 1168 50
Bsp1286I G_dGCh'C
Cuts at: 0 980 1218
Size: 980 238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1152 1218
Size: 1152 66
BstYI r'GATC_y
Cuts at: 0 414 1218
Size: 414 804
BtgI C'CryG_G
Cuts at: 0 98 452 727 1218
Size: 98 354 275 491
Fragments arranged by size:
491 354 275 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1218
Size: 388 830
EcoO109I rG'GnC_Cy
Cuts at: 0 228 1218
Size: 228 990
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 909 941 999 1020 1031 1052
Size: 15 32 862 32 58 21 11 21
Cuts at: 1052 1218
Size: 166
Fragments arranged by size:
862 166 58 32 32 21 21 15
11
HincII GTy'rAC
Cuts at: 0 247 324 962 1156 1218
Size: 247 77 638 194 62
Fragments arranged by size:
638 247 194 77 62
HpaI GTT'AAC
Cuts at: 0 962 1218
Size: 962 256
MslI CAynn'nnrTG
Cuts at: 0 602 950 1218
Size: 602 348 268
Fragments arranged by size:
602 348 268
MspA1I CmG'CkG
Cuts at: 0 790 1129 1218
Size: 790 339 89
Fragments arranged by size:
790 339 89
NcoI C'CATG_G
Cuts at: 0 98 452 727 1218
Size: 98 354 275 491
Fragments arranged by size:
491 354 275 98
NdeI CA'TA_TG
Cuts at: 0 252 1115 1218
Size: 252 863 103
Fragments arranged by size:
863 252 103
NspI r_CATG'y
Cuts at: 0 1068 1218
Size: 1068 150
PflMI CCAn_nnn'nTGG
Cuts at: 0 99 231 1218
Size: 99 132 987
Fragments arranged by size:
987 132 99
PpuMI rG'GwC_Cy
Cuts at: 0 228 1218
Size: 228 990
PstI C_TGCA'G
Cuts at: 0 342 1218
Size: 342 876
PvuII CAG'CTG
Cuts at: 0 790 1218
Size: 790 428
SalI G'TCGA_C
Cuts at: 0 322 1218
Size: 322 896
SexAI A'CCwGG_T
Cuts at: 0 230 896 1218
Size: 230 666 322
Fragments arranged by size:
666 322 230
SfcI C'TryA_G
Cuts at: 0 338 785 940 991 1218
Size: 338 447 155 51 227
Fragments arranged by size:
447 338 227 155 51
StyI C'CwwG_G
Cuts at: 0 98 452 727 1218
Size: 98 354 275 491
Fragments arranged by size:
491 354 275 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 504 536 1218
Size: 504 32 682
Fragments arranged by size:
682 504 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 78 1218
Size: 78 1140
ZraI GAC'GTC
Cuts at: 0 1178 1218
Size: 1178 40
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlfI AlwNI ApoI
BanI BbsI BciVI BclI BglI BlpI BmrI BsaAI
BsaHI BsaWI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI
BstYI BtgI Eco57MI EcoO109I Hin4I HincII HpaI MslI
MspA1I NcoI NdeI NspI PflMI PpuMI PstI PvuII
SalI SexAI SfcI StyI TstI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BfrBI BglII Bme1580I
BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XhoI XmaI XmnI