################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BcnI BglI BlnI BlpI Bpu1102I BsaHI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 153 GTCTAC GT^[AC][GT]AC AciI 828 GCGG CCGC(-3/-1) AflII 57 CTTAAG C^TTAAG Alw44I 1713 GTGCAC G^TGCAC ApaLI 1713 GTGCAC G^TGCAC AspI 1492 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1147 GGACC G^G[AT]CC BanII 1874 GAGCTC G[GA]GC[CT]^C BbrPI 1997 CACGTG CAC^GTG BbsI 2077 GAAGAC GAAGAC(2/6) BbvCI 302 CCTCAGC CCTCAGC(-5/-2) BcgI 836 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1232 GGATAC GTATCC(6/5) BclI 1502 TGATCA T^GATCA BfrI 57 CTTAAG C^TTAAG BpmI 1843 CTGGAG CTGGAG(16/14) BpuAI 2077 GAAGAC GAAGAC(2/6) BsaAI 1997 CACGTG [CT]AC^GT[GA] BsaJI 1150 CCATGG C^C[ACGT][ACGT]GG BsaWI 1451 TCCGGT [AT]^CCGG[AT] Bsp1407I 286 TGTACA T^GTACA BsrGI 286 TGTACA T^GTACA Cac8I 226 GCACGC GC[ACGT]^[ACGT]GC CfoI 1570 GCGC GCG^C Cfr13I 1147 GGACC G^G[ACGT]CC DraI 96 TTTAAA TTT^AAA DsaI 1150 CCATGG C^C[GA][CT]GG EcoNI 1617 CCTTTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1150 CCATGG C^C[AT][AT]GG FbaI 1502 TGATCA T^GATCA HaeIII 1767 GGCC GG^CC HapII 1452 CCGG C^CGG HgaI 175 GCGTC GACGC(5/10) HhaI 1570 GCGC GCG^C HinP1I 1570 GCGC G^CGC HpaII 1452 CCGG C^CGG MfeI 610 CAATTG C^AATTG MspI 1452 CCGG C^CGG MunI 610 CAATTG C^AATTG NcoI 1150 CCATGG C^CATGG NlaIV 1441 GGTTCC GG[ACGT]^[ACGT]CC NspI 866 GCATGT [GA]CATG^[CT] PflFI 1492 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1468 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1997 CACGTG CAC^GTG PmlI 1997 CACGTG CAC^GTG PstI 1352 CTGCAG CTGCA^G SacI 1874 GAGCTC GAGCT^C Sau96I 1147 GGACC G^G[ACGT]CC SfcI 1352 CTGCAG C^T[GA][CT]AG SspBI 286 TGTACA T^GTACA StyI 1150 CCATGG C^C[AT][AT]GG TspRI 1365 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 1492 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1468 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 1996 ACACGT A^C[GA][CT]GT 1998 ACGTGT AlwNI 167 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 995 CAGCTTCTG BsaBI 103 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 377 GATCTTCATC BsaI 2084 GAGACC GGTCTC(1/5) 1918 GGTCTC BseRI 426 GAGGAG GAGGAG(10/8) 837 GAGGAG BsmFI 975 GTCCC GGGAC(10/14) 1462 GTCCC EcoRI 927 GAATTC G^AATTC 1533 GAATTC HindIII 1028 AAGCTT A^AGCTT 1956 AAGCTT MaeII 944 ACGT A^CGT 1998 ACGT MamI 103 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 377 GATCTTCATC NdeI 512 CATATG CA^TATG 2038 CATATG TaqI 1044 TCGA T^CGA 2051 TCGA TthHB8I 1044 TCGA T^CGA 2051 TCGA XbaI 1195 TCTAGA T^CTAGA 1255 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I AspHI BbvI BfaI BglII BmyI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspHI BssKI BstF5I BstNI BstYI DdeI DpnII EarI EcoRII Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII PleI PvuII RcaI RsaI Sau3AI ScrFI SfaNI SmlI TfiI Tru9I TseI Tsp45I Tsp509I XhoII XmnI