(Circular) (Six-base) MAPSORT of: pFN21AE4195.seq Check: 8958 from: 1 to: 6817 pFN21AE4195 6817 bp With 182 enzymes: SgfI * September 21, 2016 11:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4627 278 Size: 53 83 186 936 3091 2468 Fragments arranged by size: 3091 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2159 4160 1937 Size: 222 2001 4594 Fragments arranged by size: 4594 2001 222 Acc65I G'GTAC_C Cuts at: 4138 4138 Size: 6817 AclI AA'CG_TT Cuts at: 1563 4944 5317 1563 Size: 3381 373 3063 Fragments arranged by size: 3381 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3027 3051 3156 3513 4019 4047 4880 6264 Size: 1395 24 105 357 506 28 833 1384 Cuts at: 6264 6532 1632 Size: 268 1917 Fragments arranged by size: 1917 1395 1384 833 506 357 268 105 28 24 AflII C'TTAA_G Cuts at: 820 1017 2062 820 Size: 197 1045 5575 Fragments arranged by size: 5575 1045 197 AflIII A'CryG_T Cuts at: 1203 4001 4003 5716 6693 1203 Size: 2798 2 1713 977 1327 Fragments arranged by size: 2798 1713 1327 977 2 AgeI A'CCGG_T Cuts at: 5648 5648 Size: 6817 AhdI GACnn_n'nnGTC Cuts at: 5546 5546 Size: 6817 AleI CACnn'nnGTG Cuts at: 1166 2493 1166 Size: 1327 5490 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6817 AlwNI CAG_nnn'CTG Cuts at: 2177 3005 4560 6132 2177 Size: 828 1555 1572 2862 Fragments arranged by size: 2862 1572 1555 828 ApaLI G'TGCA_C Cuts at: 3718 4874 6030 3718 Size: 1156 1156 4505 Fragments arranged by size: 4505 1156 1156 ApoI r'AATT_y Cuts at: 1493 2037 2662 2677 2932 2941 3118 3538 3663 Size: 544 625 15 255 9 177 420 125 Cuts at: 3663 4126 4352 4504 6613 1493 Size: 463 226 152 2109 1697 Fragments arranged by size: 2109 1697 625 544 463 420 255 226 177 152 125 15 9 AseI AT'TA_AT Cuts at: 160 1032 5371 160 Size: 872 4339 1606 Fragments arranged by size: 4339 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 AvaI C'yCGr_G Cuts at: 1870 1943 4142 1870 Size: 73 2199 4545 Fragments arranged by size: 4545 2199 73 BamHI G'GATC_C Cuts at: 4147 4514 4147 Size: 367 6450 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4138 5593 618 Size: 325 217 627 2351 1455 1842 Fragments arranged by size: 2351 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3883 4136 1391 Size: 2492 253 4072 Fragments arranged by size: 4072 2492 253 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4089 928 Size: 971 2190 3656 Fragments arranged by size: 3656 2190 971 BbvCI CC'TCA_GC Cuts at: 2308 2308 Size: 6817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2830 2864 5008 5042 1903 Size: 34 893 34 2144 34 3678 Fragments arranged by size: 3678 2144 893 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3231 4709 5919 3231 Size: 1478 1210 4129 Fragments arranged by size: 4129 1478 1210 BclI T'GATC_A Cuts at: 1548 3507 1548 Size: 1959 4858 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5428 136 Size: 107 122 71 631 605 3756 1525 Fragments arranged by size: 3756 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2381 2897 3263 4051 6812 2381 Size: 516 366 788 2761 2386 Fragments arranged by size: 2761 2386 788 516 366 BlpI GC'TnA_GC Cuts at: 4234 4234 Size: 6817 Bme1580I G_kGCm'C Cuts at: 1790 3722 4878 6034 1790 Size: 1932 1156 1156 2573 Fragments arranged by size: 2573 1932 1156 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6817 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4548 5506 442 Size: 416 53 83 399 24 3131 958 1753 Fragments arranged by size: 3131 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6817 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3869 5477 1239 Size: 2630 1608 2579 Fragments arranged by size: 2630 2579 1608 Bpu10I CC'TnA_GC Cuts at: 2308 2308 Size: 6817 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2104 2221 3390 4942 5807 6105 2104 Size: 117 1169 1552 865 298 2816 Fragments arranged by size: 2816 1552 1169 865 298 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3929 4083 5480 882 Size: 617 2430 154 1397 2219 Fragments arranged by size: 2430 2219 1397 617 154 BsaAI yAC'GTr Cuts at: 493 1204 4004 4608 493 Size: 711 2800 604 2702 Fragments arranged by size: 2800 2702 711 604 BsaBI GATnn'nnATC Cuts at: 2112 2386 6811 2112 Size: 274 4425 2118 Fragments arranged by size: 4425 2118 274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4624 5006 5641 275 Size: 53 83 186 936 3091 382 635 1451 Fragments arranged by size: 3091 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3456 5250 5648 5922 6069 6480 3456 Size: 1794 398 274 147 411 3793 Fragments arranged by size: 3793 1794 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2353 2383 1178 Size: 30 165 30 950 30 5612 Fragments arranged by size: 5612 950 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2446 2857 1186 Size: 1260 411 5146 Fragments arranged by size: 5146 1260 411 BseYI C'CCAG_C Cuts at: 2312 3472 6020 6516 2312 Size: 1160 2548 496 2613 Fragments arranged by size: 2613 2548 1160 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6244 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4267 5028 6056 1540 Size: 275 186 2266 761 1028 2301 Fragments arranged by size: 2301 2266 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2233 2582 3722 3883 4878 4963 6034 2233 Size: 349 1140 161 995 85 1071 3016 Fragments arranged by size: 3016 1140 1071 995 349 161 85 BsmI GAATG_Cn' Cuts at: 4328 4421 4328 Size: 93 6724 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2582 3722 3883 4136 4878 4963 Size: 399 443 349 1140 161 253 742 85 Cuts at: 4963 6034 1391 Size: 1071 2174 Fragments arranged by size: 2174 1140 1071 742 443 399 349 253 161 85 BspEI T'CCGG_A Cuts at: 6480 6480 Size: 6817 BspHI T'CATG_A Cuts at: 2263 3504 3865 4706 6436 2263 Size: 1241 361 841 1730 2644 Fragments arranged by size: 2644 1730 1241 841 361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4172 844 Size: 3328 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4704 1394 Size: 429 2881 3507 Fragments arranged by size: 3507 2881 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5312 5486 65 Size: 1183 4064 174 1396 Fragments arranged by size: 4064 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5461 5648 1700 Size: 110 3651 187 2869 Fragments arranged by size: 3651 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2291 96 Size: 2195 4622 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6817 BssSI C'ACGA_G Cuts at: 4877 5889 4877 Size: 1012 5805 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6817 BstBI TT'CG_AA Cuts at: 5621 5621 Size: 6817 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6817 BstYI r'GATC_y Cuts at: 1972 2381 2897 3263 3586 4051 4147 4514 4899 Size: 409 516 366 323 465 96 367 385 Cuts at: 4899 4916 6368 6454 6466 6812 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 516 465 409 385 367 366 346 323 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3155 513 Size: 552 939 1151 4175 Fragments arranged by size: 4175 1151 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6078 Fragments arranged by size: 6078 683 56 BtsI GCAGTG_nn' Cuts at: 3376 4333 5127 5147 3376 Size: 957 794 20 5046 Fragments arranged by size: 5046 957 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6817 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3276 3311 529 Size: 35 2712 35 4035 Fragments arranged by size: 4035 2712 35 35 DraI TTT'AAA Cuts at: 2103 4121 2103 Size: 2018 4799 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5824 809 Size: 507 4508 1802 Fragments arranged by size: 4508 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4264 5153 6782 Size: 54 1425 50 275 18 2434 889 1629 Cuts at: 6782 8 Size: 43 Fragments arranged by size: 2434 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4264 1537 Size: 275 2452 4090 Fragments arranged by size: 4090 2452 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2134 3214 3274 4038 4747 1353 Size: 781 1080 60 764 709 3423 Fragments arranged by size: 3423 1080 781 764 709 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2848 5404 5778 5924 387 Size: 1064 138 1259 2556 374 146 1280 Fragments arranged by size: 2556 1280 1259 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3881 3881 Size: 6817 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3027 3051 3156 3513 3869 4019 4047 Size: 393 1395 24 105 357 356 150 28 Cuts at: 4047 4880 5477 6264 6532 1239 Size: 833 597 787 268 1524 Fragments arranged by size: 1524 1395 833 787 597 393 357 356 268 150 105 28 24 EcoNI CCTnn'n_nnAGG Cuts at: 3626 3626 Size: 6817 EcoRI G'AATT_C Cuts at: 2932 3538 4126 4504 2932 Size: 606 588 378 5245 Fragments arranged by size: 5245 606 588 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2145 3594 2145 Size: 1449 5368 FspI TGC'GCA Cuts at: 5323 5323 Size: 6817 HaeII r_GCGC'y Cuts at: 1678 5964 1678 Size: 4286 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2353 2385 3242 3274 5458 Size: 32 135 32 781 32 857 32 2184 Cuts at: 5458 5490 5532 5564 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2184 857 781 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4161 4407 669 Size: 988 281 2223 246 3079 Fragments arranged by size: 3079 2223 988 281 246 HindIII A'AGCT_T Cuts at: 748 3033 3961 748 Size: 2285 928 3604 Fragments arranged by size: 3604 2285 928 HpaI GTT'AAC Cuts at: 4407 4407 Size: 6817 KpnI G_GTAC'C Cuts at: 4142 4142 Size: 6817 MfeI C'AATT_G Cuts at: 2615 4416 2615 Size: 1801 5016 MluI A'CGCG_T Cuts at: 6693 6693 Size: 6817 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2305 3515 3816 5931 6115 1305 Size: 1000 1210 301 2115 184 2007 Fragments arranged by size: 2115 2007 1210 1000 301 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4995 Fragments arranged by size: 4995 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2493 3715 3888 4775 5134 5293 518 Size: 648 1327 1222 173 887 359 159 2042 Fragments arranged by size: 2042 1327 1222 887 648 359 173 159 MspA1I CmG'CkG Cuts at: 1606 2312 3545 3680 4233 4910 6058 6303 1606 Size: 706 1233 135 553 677 1148 245 2120 Fragments arranged by size: 2120 1233 1148 706 677 553 245 135 NcoI C'CATG_G Cuts at: 513 1065 2004 3155 513 Size: 552 939 1151 4175 Fragments arranged by size: 4175 1151 939 552 NdeI CA'TA_TG Cuts at: 387 2518 4044 387 Size: 2131 1526 3160 Fragments arranged by size: 3160 2131 1526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6817 NotI GC'GGCC_GC Cuts at: 4264 4264 Size: 6817 NspI r_CATG'y Cuts at: 1132 1229 2875 4175 5720 1132 Size: 97 1646 1300 1545 2229 Fragments arranged by size: 2229 1646 1545 1300 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6817 PciI A'CATG_T Cuts at: 5716 5716 Size: 6817 PflMI CCAn_nnn'nTGG Cuts at: 1108 3479 1108 Size: 2371 4446 PmeI GTTT'AAAC Cuts at: 4121 4121 Size: 6817 PmlI CAC'GTG Cuts at: 4004 4004 Size: 6817 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4886 4918 6399 6431 4886 Size: 32 1481 32 5272 Fragments arranged by size: 5272 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6817 PsiI TTA'TAA Cuts at: 4387 4387 Size: 6817 PstI C_TGCA'G Cuts at: 830 3361 4169 830 Size: 2531 808 3478 Fragments arranged by size: 3478 2531 808 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6817 PvuII CAG'CTG Cuts at: 2312 3545 3680 2312 Size: 1233 135 5449 Fragments arranged by size: 5449 1233 135 SacI G_AGCT'C Cuts at: 3883 3883 Size: 6817 SalI G'TCGA_C Cuts at: 1936 4159 1936 Size: 2223 4594 SbfI CC_TGCA'GG Cuts at: 4169 4169 Size: 6817 ScaI AGT'ACT Cuts at: 5065 6777 5065 Size: 1712 5105 SfcI C'TryA_G Cuts at: 826 1045 3357 4165 5300 5981 6172 826 Size: 219 2312 808 1135 681 191 1471 Fragments arranged by size: 2312 1471 1135 808 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6817 SmaI CCC'GGG Cuts at: 1872 4144 1872 Size: 2272 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2062 2119 2236 3405 4921 5822 Size: 197 926 119 57 117 1169 1516 901 Cuts at: 5822 6084 820 Size: 262 1553 Fragments arranged by size: 1553 1516 1169 926 901 262 197 119 117 57 SnaBI TAC'GTA Cuts at: 493 4608 493 Size: 4115 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6817 SphI G_CATG'C Cuts at: 1132 4175 1132 Size: 3043 3774 SspI AAT'ATT Cuts at: 5 52 4741 5 Size: 47 4689 2081 Fragments arranged by size: 4689 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6817 StyI C'CwwG_G Cuts at: 513 1065 2004 3155 4256 513 Size: 552 939 1151 1101 3074 Fragments arranged by size: 3074 1151 1101 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3509 4844 4861 5014 5199 5644 325 Size: 1255 1929 1335 17 153 185 445 1498 Fragments arranged by size: 1929 1498 1335 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2291 2300 5063 Size: 274 80 33 51 1443 314 9 2763 Cuts at: 5063 6775 96 Size: 1712 138 Fragments arranged by size: 2763 1712 1443 314 274 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6777 6809 6777 Size: 32 6785 Tth111I GACn'n_nGTC Cuts at: 1316 3500 1316 Size: 2184 4633 XbaI T'CTAG_A Cuts at: 3200 3260 4153 3200 Size: 60 893 5864 Fragments arranged by size: 5864 893 60 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6817 XmaI C'CCGG_G Cuts at: 1870 4142 1870 Size: 2272 4545 XmnI GAAnn'nnTTC Cuts at: 2038 2455 3297 4946 2038 Size: 417 842 1649 3909 Fragments arranged by size: 3909 1649 842 417 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4625 276 Size: 53 83 186 936 3091 2468 Fragments arranged by size: 3091 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI