(Linear) (Six-base) MAPSORT of: KIEE4195.seq Check: 1791 from: 1 to: 2112 KIEE4195.orf With 182 enzymes: SgfI * September 21, 2016 11:20 .. AccI GT'mk_AC Cuts at: 0 154 2112 Size: 154 1958 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1022 1046 1151 1508 2014 2042 2112 Size: 1022 24 105 357 506 28 70 Fragments arranged by size: 1022 506 357 105 70 28 24 AflII C'TTAA_G Cuts at: 0 57 2112 Size: 57 2055 AflIII A'CryG_T Cuts at: 0 1996 1998 2112 Size: 1996 2 114 Fragments arranged by size: 1996 114 2 AleI CACnn'nnGTG Cuts at: 0 488 2112 Size: 488 1624 AlwNI CAG_nnn'CTG Cuts at: 0 172 1000 2112 Size: 172 828 1112 Fragments arranged by size: 1112 828 172 ApaLI G'TGCA_C Cuts at: 0 1713 2112 Size: 1713 399 ApoI r'AATT_y Cuts at: 0 32 657 672 927 936 1113 1533 1658 Size: 32 625 15 255 9 177 420 125 Cuts at: 1658 2112 Size: 454 Fragments arranged by size: 625 454 420 255 177 125 32 15 9 BanII G_rGCy'C Cuts at: 0 1878 2112 Size: 1878 234 BbsI GAAGACnn'nnnn_ Cuts at: 0 2084 2112 Size: 2084 28 BbvCI CC'TCA_GC Cuts at: 0 303 2112 Size: 303 1809 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 825 859 2112 Size: 825 34 1253 Fragments arranged by size: 1253 825 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1226 2112 Size: 1226 886 BclI T'GATC_A Cuts at: 0 1502 2112 Size: 1502 610 BglII A'GATC_T Cuts at: 0 376 892 1258 2046 2112 Size: 376 516 366 788 66 Fragments arranged by size: 788 516 376 366 66 Bme1580I G_kGCm'C Cuts at: 0 1717 2112 Size: 1717 395 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1864 2112 Size: 1864 248 Bpu10I CC'TnA_GC Cuts at: 0 303 2112 Size: 303 1809 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 99 216 1385 2112 Size: 99 117 1169 727 Fragments arranged by size: 1169 727 117 99 BsaI GGTCTCn'nnnn_ Cuts at: 0 1924 2078 2112 Size: 1924 154 34 Fragments arranged by size: 1924 154 34 BsaAI yAC'GTr Cuts at: 0 1999 2112 Size: 1999 113 BsaBI GATnn'nnATC Cuts at: 0 107 381 2112 Size: 107 274 1731 Fragments arranged by size: 1731 274 107 BsaWI w'CCGG_w Cuts at: 0 1451 2112 Size: 1451 661 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 348 378 2112 Size: 348 30 1734 Fragments arranged by size: 1734 348 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 441 852 2112 Size: 441 411 1260 Fragments arranged by size: 1260 441 411 BseYI C'CCAG_C Cuts at: 0 307 1467 2112 Size: 307 1160 645 Fragments arranged by size: 1160 645 307 BsiHKAI G_wGCw'C Cuts at: 0 228 577 1717 1878 2112 Size: 228 349 1140 161 234 Fragments arranged by size: 1140 349 234 228 161 Bsp1286I G_dGCh'C Cuts at: 0 228 577 1717 1878 2112 Size: 228 349 1140 161 234 Fragments arranged by size: 1140 349 234 228 161 BspHI T'CATG_A Cuts at: 0 258 1499 1860 2112 Size: 258 1241 361 252 Fragments arranged by size: 1241 361 258 252 BsrGI T'GTAC_A Cuts at: 0 286 2112 Size: 286 1826 BstYI r'GATC_y Cuts at: 0 376 892 1258 1581 2046 2112 Size: 376 516 366 323 465 66 Fragments arranged by size: 516 465 376 366 323 66 BtgI C'CryG_G Cuts at: 0 1150 2112 Size: 1150 962 BtsI GCAGTG_nn' Cuts at: 0 1371 2112 Size: 1371 741 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1271 1306 2112 Size: 1271 35 806 Fragments arranged by size: 1271 806 35 DraI TTT'AAA Cuts at: 0 98 2112 Size: 98 2014 EarI CTCTTCn'nnn_ Cuts at: 0 129 1209 1269 2033 2112 Size: 129 1080 60 764 79 Fragments arranged by size: 1080 764 129 79 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 843 2112 Size: 843 1269 EcoICRI GAG'CTC Cuts at: 0 1876 2112 Size: 1876 236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1022 1046 1151 1508 1864 2014 2042 2112 Size: 1022 24 105 357 356 150 28 70 Fragments arranged by size: 1022 357 356 150 105 70 28 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 1621 2112 Size: 1621 491 EcoRI G'AATT_C Cuts at: 0 927 1533 2112 Size: 927 606 579 Fragments arranged by size: 927 606 579 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 140 1589 2112 Size: 140 1449 523 Fragments arranged by size: 1449 523 140 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 348 380 1237 1269 2112 Size: 348 32 857 32 843 Fragments arranged by size: 857 843 348 32 32 HindIII A'AGCT_T Cuts at: 0 1028 1956 2112 Size: 1028 928 156 Fragments arranged by size: 1028 928 156 MfeI C'AATT_G Cuts at: 0 610 2112 Size: 610 1502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 300 1510 1811 2112 Size: 300 1210 301 301 Fragments arranged by size: 1210 301 301 300 MslI CAynn'nnrTG Cuts at: 0 488 1710 1883 2112 Size: 488 1222 173 229 Fragments arranged by size: 1222 488 229 173 MspA1I CmG'CkG Cuts at: 0 307 1540 1675 2112 Size: 307 1233 135 437 Fragments arranged by size: 1233 437 307 135 NcoI C'CATG_G Cuts at: 0 1150 2112 Size: 1150 962 NdeI CA'TA_TG Cuts at: 0 513 2039 2112 Size: 513 1526 73 Fragments arranged by size: 1526 513 73 NspI r_CATG'y Cuts at: 0 870 2112 Size: 870 1242 PflMI CCAn_nnn'nTGG Cuts at: 0 1474 2112 Size: 1474 638 PmlI CAC'GTG Cuts at: 0 1999 2112 Size: 1999 113 PstI C_TGCA'G Cuts at: 0 1356 2112 Size: 1356 756 PvuII CAG'CTG Cuts at: 0 307 1540 1675 2112 Size: 307 1233 135 437 Fragments arranged by size: 1233 437 307 135 SacI G_AGCT'C Cuts at: 0 1878 2112 Size: 1878 234 SfcI C'TryA_G Cuts at: 0 1352 2112 Size: 1352 760 SmlI C'TyrA_G Cuts at: 0 57 114 231 1400 2112 Size: 57 57 117 1169 712 Fragments arranged by size: 1169 712 117 57 57 StyI C'CwwG_G Cuts at: 0 1150 2112 Size: 1150 962 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1504 2112 Size: 1504 608 TatI w'GTAC_w Cuts at: 0 286 295 2112 Size: 286 9 1817 Fragments arranged by size: 1817 286 9 Tth111I GACn'n_nGTC Cuts at: 0 1495 2112 Size: 1495 617 XbaI T'CTAG_A Cuts at: 0 1195 1255 2112 Size: 1195 60 857 Fragments arranged by size: 1195 857 60 XmnI GAAnn'nnTTC Cuts at: 0 33 450 1292 2112 Size: 33 417 842 820 Fragments arranged by size: 842 820 417 33 Enzymes that do cut: AccI AcuI AflII AflIII AleI AlwNI ApaLI ApoI BanII BbsI BbvCI BcgI BciVI BclI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI BsrGI BstYI BtgI BtsI CspCI DraI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI Hin4I HindIII MfeI MmeI MslI MspA1I NcoI NdeI NspI PflMI PmlI PstI PvuII SacI SfcI SmlI StyI TaqII TatI Tth111I XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BfrBI BglI BlpI BmgBI BmrI BmtI BplI BsaHI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraIII DrdI EaeI EagI EcoO109I EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XcmI XhoI XmaI ZraI