Restriction map for
pFN21AE2449
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp718I AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII HhaI HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1396 GTCTAC GT^[AC][GT]AC
AclI 1780 AACGTT AA^CGTT
AcyI 1325 GGCGTC G[GA]^CG[CT]C
AhdI 654 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 231 CCTAAGG CC^T[ACGT]AGG
AspEI 654 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2283 TGGCCA TGG^CCA
BamHI 8 GGATCC G^GATCC
BcnI 590 CCCGG CC^[GC]GG
BglII 1540 AGATCT A^GATCT
BsaHI 1325 GGCGTC G[GA]^CG[CT]C
BsaWI 2207 ACCGGA [AT]^CCGG[AT]
BspLU11I 1649 ACATGT A^CATGT
BspMI 1446 ACCTGC ACCTGC(4/8)
BsrDI 1959 CATTGC GCAATG(2/0)
Bsu36I 231 CCTAAGG CC^T[ACGT]AGG
DraI 576 TTTAAA TTT^AAA
DraII 1298 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1261 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2283 TGGCCA [CT]^GGCC[GA]
Eam1105I 654 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 231 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1298 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 2463 ATGCAT ATGCA^T
FauI 2238 GCGGG CCCGC(4/6)
HgaI 1326 GCGTC GACGC(5/10)
Hin1I 1325 GGCGTC G[GA]^CG[CT]C
HincII 1749 GTTGAC GT[CT]^[GA]AC
HindII 1749 GTTGAC GT[CT]^[GA]AC
MluNI 2283 TGGCCA TGG^CCA
MscI 2283 TGGCCA TGG^CCA
MspA1I 632 CAGCTG C[AC]G^C[GT]G
NciI 590 CCCGG CC^[GC]GG
NsiI 2463 ATGCAT ATGCA^T
NspI 1649 ACATGT [GA]CATG^[CT]
PpuMI 1298 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1780 AACGTT AA^CGTT
PstI 394 CTGCAG CTGCA^G
PvuII 632 CAGCTG CAG^CTG
SacI 2101 GAGCTC GAGCT^C
SapI 985 GAAGAGC GCTCTTC(1/4)
ScaI 667 AGTACT AGT^ACT
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 861 CTTAAG C^TTAAG
2406 CTTAAG
AflIII 567 ACGTGT A^C[GA][CT]GT
1649 ACATGT
AseI 877 ATTAAT AT^TAAT
2443 ATTAAT
AsnI 877 ATTAAT AT^TAAT
2443 ATTAAT
Asp700I 1795 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2732 GAAATTTTTC
AvaI 125 CTCGGG C^[CT]CG[GA]G
2156 CCCGAG
BanI 442 GGCACC G^G[CT][GA]CC
1997 GGTGCC
BfrI 861 CTTAAG C^TTAAG
2406 CTTAAG
BglI 372 GCCGTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1711 GCCAGGCTGGC
BsaI 2291 GAGACC GGTCTC(1/5)
1011 GGTCTC
BsmI 345 GAATGC GAATGC(1/-1)
1731 GAATGC
BsoBI 125 CTCGGG C^[CT]CG[GA]G
2156 CCCGAG
BsrBI 157 GAGCGG CCGCTC(-3/-3)
2224 GAGCGG
BstAPI 715 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1726 GCACAGAATGC
EcoNI 1578 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2587 CCTGATAAAGG
HapII 591 CCGG C^CGG
2208 CCGG
HpaII 591 CCGG C^CGG
2208 CCGG
MaeII 567 ACGT A^CGT
1781 ACGT
MspI 591 CCGG C^CGG
2208 CCGG
PshBI 877 ATTAAT AT^TAAT
2443 ATTAAT
SexAI 1262 ACCAGGT A^CC[AT]GGT
2539 ACCTGGT
SfaNI 381 GCATC GCATC(5/9)
1907 GCATC
SspI 1035 AATATT AAT^ATT
2807 AATATT
TaqI 1850 TCGA T^CGA
2303 TCGA
TthHB8I 1850 TCGA T^CGA
2303 TCGA
XcmI 1146 CCATGCTTTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2782 CCATATCAAAAGTGG
XmnI 1795 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2732 GAAATTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII