(Circular) (Six-base) MAPSORT of: pFN21AE2449.seq Check: 9878 from: 1 to: 7525 pFN21AE2449 7525 bp With 182 enzymes: SgfI * January 18, 2013 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5335 278 Size: 53 83 186 936 3799 2468 Fragments arranged by size: 3799 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3402 4868 1937 Size: 1465 1466 4594 Fragments arranged by size: 4594 1466 1465 Acc65I G'GTAC_C Cuts at: 4846 4846 Size: 7525 AclI AA'CG_TT Cuts at: 1563 3786 5652 6025 1563 Size: 2223 1866 373 3063 Fragments arranged by size: 3063 2223 1866 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2478 3270 3598 4385 5588 6972 7240 1632 Size: 846 792 328 787 1203 1384 268 1917 Fragments arranged by size: 1917 1384 1203 846 792 787 328 268 AflII C'TTAA_G Cuts at: 820 1017 2866 4411 820 Size: 197 1849 1545 3934 Fragments arranged by size: 3934 1849 1545 197 AflIII A'CryG_T Cuts at: 1203 2572 3654 6424 7401 1203 Size: 1369 1082 2770 977 1327 Fragments arranged by size: 2770 1369 1327 1082 977 AgeI A'CCGG_T Cuts at: 6356 6356 Size: 7525 AhdI GACnn_n'nnGTC Cuts at: 2664 6254 2664 Size: 3590 3935 AleI CACnn'nnGTG Cuts at: 1166 2738 4620 1166 Size: 1572 1882 4071 Fragments arranged by size: 4071 1882 1572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2390 2756 1184 Size: 1206 366 5953 Fragments arranged by size: 5953 1206 366 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2163 2195 3937 3969 2163 Size: 32 1742 32 5719 Fragments arranged by size: 5719 1742 32 32 AlwNI CAG_nnn'CTG Cuts at: 5268 6840 5268 Size: 1572 5953 ApaLI G'TGCA_C Cuts at: 5582 6738 5582 Size: 1156 6369 ApoI r'AATT_y Cuts at: 1493 2199 4738 4802 4834 5060 5212 7321 1493 Size: 706 2539 64 32 226 152 2109 1697 Fragments arranged by size: 2539 2109 1697 706 226 152 64 32 AseI AT'TA_AT Cuts at: 160 1032 2883 4449 6079 160 Size: 872 1851 1566 1630 1606 Fragments arranged by size: 1851 1630 1606 1566 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7525 AvaI C'yCGr_G Cuts at: 1870 1943 2130 4161 4850 1870 Size: 73 187 2031 689 4545 Fragments arranged by size: 4545 2031 689 187 73 BamHI G'GATC_C Cuts at: 2013 4855 5222 2013 Size: 2842 367 4316 Fragments arranged by size: 4316 2842 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2447 4002 4846 6301 618 Size: 325 217 627 660 1555 844 1455 1842 Fragments arranged by size: 1842 1555 1455 844 660 627 325 217 BanII G_rGCy'C Cuts at: 1391 2509 2595 2935 4110 4420 4844 1391 Size: 1118 86 340 1175 310 424 4072 Fragments arranged by size: 4072 1175 1118 424 340 310 86 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2151 2438 2822 3096 3258 3447 4226 Size: 971 252 287 384 274 162 189 779 Cuts at: 4226 4509 928 Size: 283 3944 Fragments arranged by size: 3944 971 779 384 287 283 274 252 189 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5716 5750 1903 Size: 34 3779 34 3678 Fragments arranged by size: 3779 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5417 6627 5417 Size: 1210 6315 BclI T'GATC_A Cuts at: 1548 1548 Size: 7525 BfrBI ATG'CAT Cuts at: 4470 4470 Size: 7525 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2383 3722 6136 Size: 107 122 71 631 605 711 1339 2414 Cuts at: 6136 136 Size: 1525 Fragments arranged by size: 2414 1525 1339 711 631 605 122 107 71 BglII A'GATC_T Cuts at: 3545 7520 3545 Size: 3975 3550 BlpI GC'TnA_GC Cuts at: 4942 4942 Size: 7525 Bme1580I G_kGCm'C Cuts at: 1790 2722 4007 5586 6742 1790 Size: 932 1285 1579 1156 2573 Fragments arranged by size: 2573 1579 1285 1156 932 BmgBI CAC'GTC Cuts at: 1318 2573 1318 Size: 1255 6270 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3416 3655 4772 Size: 416 53 83 399 24 1999 239 1117 Cuts at: 4772 5256 6214 442 Size: 484 958 1753 Fragments arranged by size: 1999 1753 1117 958 484 416 399 239 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7525 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4319 4319 Size: 7525 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3183 3300 3939 6185 1239 Size: 1944 117 639 2246 2579 Fragments arranged by size: 2579 2246 1944 639 117 Bpu10I CC'TnA_GC Cuts at: 2499 3407 2499 Size: 908 6617 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2285 5650 6515 6813 2285 Size: 3365 865 298 2997 Fragments arranged by size: 3365 2997 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3022 4290 6188 882 Size: 617 1523 1268 1898 2219 Fragments arranged by size: 2219 1898 1523 1268 617 BsaAI yAC'GTr Cuts at: 493 1204 5316 493 Size: 711 4112 2702 Fragments arranged by size: 4112 2702 711 BsaBI GATnn'nnATC Cuts at: 7519 7519 Size: 7525 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3331 5332 5714 6349 Size: 53 83 186 936 1798 2001 382 635 Cuts at: 6349 275 Size: 1451 Fragments arranged by size: 2001 1798 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4212 5958 6356 6630 6777 7188 4212 Size: 1746 398 274 147 411 4549 Fragments arranged by size: 4549 1746 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3280 3310 3937 3967 1178 Size: 30 165 30 1877 30 627 30 4736 Fragments arranged by size: 4736 1877 627 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2170 2303 2455 3564 1186 Size: 886 98 133 152 1109 5147 Fragments arranged by size: 5147 1109 886 152 133 98 BseYI C'CCAG_C Cuts at: 2612 4338 6728 7224 2612 Size: 1726 2390 496 2913 Fragments arranged by size: 2913 2390 1726 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2376 3483 4598 1163 Size: 573 640 1107 1115 4090 Fragments arranged by size: 4090 1115 1107 640 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4975 5736 6764 1540 Size: 275 186 2974 761 1028 2301 Fragments arranged by size: 2974 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2301 3733 4110 5586 5671 6742 2301 Size: 1432 377 1476 85 1071 3084 Fragments arranged by size: 3084 1476 1432 1071 377 85 BsmI GAATG_Cn' Cuts at: 2356 3742 5036 5129 2356 Size: 1386 1294 93 4752 Fragments arranged by size: 4752 1386 1294 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2509 2595 2722 2935 3733 4007 Size: 399 511 208 86 127 213 798 274 Cuts at: 4007 4110 4420 4844 5586 5671 6742 1391 Size: 103 310 424 742 85 1071 2174 Fragments arranged by size: 2174 1071 798 742 511 424 399 310 274 213 208 127 103 86 85 BspEI T'CCGG_A Cuts at: 7188 7188 Size: 7525 BspHI T'CATG_A Cuts at: 5414 7144 5414 Size: 1730 5795 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3460 4880 844 Size: 2616 1420 3489 Fragments arranged by size: 3489 2616 1420 BsrBI CCG'CTC Cuts at: 1394 1823 2164 4231 5412 1394 Size: 429 341 2067 1181 3507 Fragments arranged by size: 3507 2067 1181 429 341 BsrDI GCAATG_nn' Cuts at: 65 1248 3963 6020 6194 65 Size: 1183 2715 2057 174 1396 Fragments arranged by size: 2715 2057 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6169 6356 1700 Size: 110 4359 187 2869 Fragments arranged by size: 4359 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7525 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7525 BssSI C'ACGA_G Cuts at: 5585 6597 5585 Size: 1012 6513 BstAPI GCAn_nnn'nTGC Cuts at: 794 2726 3737 794 Size: 1932 1011 4582 Fragments arranged by size: 4582 1932 1011 BstBI TT'CG_AA Cuts at: 6329 6329 Size: 7525 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7525 BstYI r'GATC_y Cuts at: 1972 2013 3545 4752 4855 5222 5607 5624 7076 Size: 41 1532 1207 103 367 385 17 1452 Cuts at: 7076 7162 7174 7520 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1532 1452 1207 385 367 346 103 86 41 17 12 Bsu36I CC'TnA_GG Cuts at: 2237 2237 Size: 7525 BtgI C'CryG_G Cuts at: 513 1065 2760 3349 4291 513 Size: 552 1695 589 942 3747 Fragments arranged by size: 3747 1695 942 589 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4524 487 Size: 683 56 3298 3488 Fragments arranged by size: 3488 3298 683 56 BtsI GCAGTG_nn' Cuts at: 2623 2745 5041 5835 5855 2623 Size: 122 2296 794 20 4293 Fragments arranged by size: 4293 2296 794 122 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7525 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7490 DraI TTT'AAA Cuts at: 2583 4829 2583 Size: 2246 5279 DraIII CAC_nnn'GTG Cuts at: 3271 3271 Size: 7525 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6532 809 Size: 507 5216 1802 Fragments arranged by size: 5216 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4288 4972 5861 Size: 54 1425 50 275 18 2458 684 889 Cuts at: 5861 7490 8 Size: 1629 43 Fragments arranged by size: 2458 1629 1425 889 684 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4972 1537 Size: 275 3160 4090 Fragments arranged by size: 4090 3160 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2594 2985 3115 3810 5455 1353 Size: 1241 391 130 695 1645 3423 Fragments arranged by size: 3423 1645 1241 695 391 130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 6112 6486 6632 387 Size: 1064 138 504 4019 374 146 1280 Fragments arranged by size: 4019 1280 1064 504 374 146 138 EcoICRI GAG'CTC Cuts at: 4108 4108 Size: 7525 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2478 3183 3270 3300 3598 3939 4385 Size: 393 846 705 87 30 298 341 446 Cuts at: 4385 5588 6185 6972 7240 1239 Size: 1203 597 787 268 1524 Fragments arranged by size: 1524 1203 846 787 705 597 446 393 341 298 268 87 30 EcoNI CCTnn'n_nnAGG Cuts at: 3587 4596 3587 Size: 1009 6516 EcoO109I rG'GnC_Cy Cuts at: 3304 3304 Size: 7525 EcoRI G'AATT_C Cuts at: 4834 5212 4834 Size: 378 7147 FspI TGC'GCA Cuts at: 6031 6031 Size: 7525 HaeII r_GCGC'y Cuts at: 1678 6672 1678 Size: 4994 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2113 2145 2215 2247 2593 Size: 32 135 32 541 32 70 32 346 Cuts at: 2593 2625 2650 2682 3280 3312 6166 6198 6240 Size: 32 25 32 598 32 2854 32 42 Cuts at: 6240 6272 1373 Size: 32 2626 Fragments arranged by size: 2854 2626 598 541 346 135 70 42 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3756 4869 5115 669 Size: 988 281 1818 1113 246 3079 Fragments arranged by size: 3079 1818 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7525 HpaI GTT'AAC Cuts at: 5115 5115 Size: 7525 KpnI G_GTAC'C Cuts at: 4850 4850 Size: 7525 MfeI C'AATT_G Cuts at: 5124 5124 Size: 7525 MluI A'CGCG_T Cuts at: 7401 7401 Size: 7525 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3777 3920 6639 6823 1305 Size: 2472 143 2719 184 2007 Fragments arranged by size: 2719 2472 2007 184 143 MscI TGG'CCA Cuts at: 10 64 1489 1832 4290 10 Size: 54 1425 343 2458 3245 Fragments arranged by size: 3245 2458 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 3890 3917 4620 5483 5842 6001 Size: 648 1572 1152 27 703 863 359 159 Cuts at: 6001 518 Size: 2042 Fragments arranged by size: 2042 1572 1152 863 703 648 359 159 27 MspA1I CmG'CkG Cuts at: 1606 2639 4941 5618 6766 7011 1606 Size: 1033 2302 677 1148 245 2120 Fragments arranged by size: 2302 2120 1148 1033 677 245 NcoI C'CATG_G Cuts at: 513 1065 2760 3349 4291 513 Size: 552 1695 589 942 3747 Fragments arranged by size: 3747 1695 942 589 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7525 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7525 NotI GC'GGCC_GC Cuts at: 4972 4972 Size: 7525 NsiI A_TGCA'T Cuts at: 4472 4472 Size: 7525 NspI r_CATG'y Cuts at: 1132 1229 3658 4883 6428 1132 Size: 97 2429 1225 1545 2229 Fragments arranged by size: 2429 2229 1545 1225 97 PasI CC'CwG_GG Cuts at: 1348 2356 1348 Size: 1008 6517 PciI A'CATG_T Cuts at: 3654 6424 3654 Size: 2770 4755 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7525 PfoI T'CCnGG_A Cuts at: 3083 3083 Size: 7525 PmeI GTTT'AAAC Cuts at: 4829 4829 Size: 7525 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3937 3969 5594 5626 7107 7139 3937 Size: 32 1625 32 1481 32 4323 Fragments arranged by size: 4323 1625 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3304 3304 Size: 7525 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7525 PsiI TTA'TAA Cuts at: 2978 5095 2978 Size: 2117 5408 PstI C_TGCA'G Cuts at: 830 2403 4877 830 Size: 1573 2474 3478 Fragments arranged by size: 3478 2474 1573 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7525 PvuII CAG'CTG Cuts at: 2639 2639 Size: 7525 SacI G_AGCT'C Cuts at: 4110 4110 Size: 7525 SalI G'TCGA_C Cuts at: 1936 4867 1936 Size: 2931 4594 SanDI GG'GwC_CC Cuts at: 3304 3304 Size: 7525 SapI GCTCTTCn'nnn_ Cuts at: 2985 2985 Size: 7525 SbfI CC_TGCA'GG Cuts at: 4877 4877 Size: 7525 ScaI AGT'ACT Cuts at: 2674 5773 7485 2674 Size: 3099 1712 2714 Fragments arranged by size: 3099 2714 1712 SexAI A'CCwGG_T Cuts at: 3267 4544 3267 Size: 1277 6248 SfcI C'TryA_G Cuts at: 826 1045 2399 3210 3336 4488 4873 6008 6689 Size: 219 1354 811 126 1152 385 1135 681 Cuts at: 6689 6880 826 Size: 191 1471 Fragments arranged by size: 1471 1354 1152 1135 811 681 385 219 191 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7525 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7525 SmaI CCC'GGG Cuts at: 1872 4852 1872 Size: 2980 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2300 2866 4411 5629 6530 6792 Size: 197 926 357 566 1545 1218 901 262 Cuts at: 6792 820 Size: 1553 Fragments arranged by size: 1553 1545 1218 926 901 566 357 262 197 SnaBI TAC'GTA Cuts at: 493 5316 493 Size: 4823 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7525 SphI G_CATG'C Cuts at: 1132 4883 1132 Size: 3751 3774 SspI AAT'ATT Cuts at: 5 52 3042 4814 5449 5 Size: 47 2990 1772 635 2081 Fragments arranged by size: 2990 2081 1772 635 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7525 StyI C'CwwG_G Cuts at: 513 1065 2191 2760 3299 3349 4291 4312 4964 Size: 552 1126 569 539 50 942 21 652 Cuts at: 4964 513 Size: 3074 Fragments arranged by size: 3074 1126 942 652 569 552 539 50 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4352 5552 5569 5722 5907 6352 325 Size: 1255 2772 1200 17 153 185 445 1498 Fragments arranged by size: 2772 1498 1255 1200 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2560 2672 5771 Size: 274 80 33 51 1443 583 112 3099 Cuts at: 5771 7483 96 Size: 1712 138 Fragments arranged by size: 3099 1712 1443 583 274 138 112 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3079 3111 3512 3544 4117 4149 7485 7517 3079 Size: 32 401 32 573 32 3336 32 3087 Fragments arranged by size: 3336 3087 573 401 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7525 XbaI T'CTAG_A Cuts at: 4861 4861 Size: 7525 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3158 4794 3158 Size: 1636 5889 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7525 XmaI C'CCGG_G Cuts at: 1870 4850 1870 Size: 2980 4545 XmnI GAAnn'nnTTC Cuts at: 3804 4741 5654 3804 Size: 937 913 5675 Fragments arranged by size: 5675 937 913 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5333 276 Size: 53 83 186 936 3799 2468 Fragments arranged by size: 3799 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI