Restriction map for
pFN21AE2449
(Linear) (Six-base) MAPSORT of: KIEE2449.seq Check: 2819 from: 1 to: 2820
KIEE2449.orf
With 182 enzymes: SgfI *
January 18, 2013 16:22 ..
AccI GT'mk_AC
Cuts at: 0 1397 2820
Size: 1397 1423
AclI AA'CG_TT
Cuts at: 0 1781 2820
Size: 1781 1039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1265 1593 2380 2820
Size: 473 792 328 787 440
Fragments arranged by size:
792 787 473 440 328
AflII C'TTAA_G
Cuts at: 0 861 2406 2820
Size: 861 1545 414
Fragments arranged by size:
1545 861 414
AflIII A'CryG_T
Cuts at: 0 567 1649 2820
Size: 567 1082 1171
Fragments arranged by size:
1171 1082 567
AhdI GACnn_n'nnGTC
Cuts at: 0 659 2820
Size: 659 2161
AleI CACnn'nnGTG
Cuts at: 0 733 2615 2820
Size: 733 1882 205
Fragments arranged by size:
1882 733 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 385 751 2820
Size: 385 366 2069
Fragments arranged by size:
2069 385 366
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 1932 1964 2820
Size: 158 32 1742 32 856
Fragments arranged by size:
1742 856 158 32 32
ApoI r'AATT_y
Cuts at: 0 194 2733 2797 2820
Size: 194 2539 64 23
Fragments arranged by size:
2539 194 64 23
AseI AT'TA_AT
Cuts at: 0 878 2444 2820
Size: 878 1566 376
Fragments arranged by size:
1566 878 376
AvaI C'yCGr_G
Cuts at: 0 125 2156 2820
Size: 125 2031 664
Fragments arranged by size:
2031 664 125
BamHI G'GATC_C
Cuts at: 0 8 2820
Size: 8 2812
BanI G'GyrC_C
Cuts at: 0 442 1997 2820
Size: 442 1555 823
Fragments arranged by size:
1555 823 442
BanII G_rGCy'C
Cuts at: 0 504 590 930 2105 2415 2820
Size: 504 86 340 1175 310 405
Fragments arranged by size:
1175 504 405 340 310 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 433 817 1091 1253 1442 2221 2504
Size: 146 287 384 274 162 189 779 283
Cuts at: 2504 2820
Size: 316
Fragments arranged by size:
779 384 316 287 283 274 189 162
146
BfrBI ATG'CAT
Cuts at: 0 2465 2820
Size: 2465 355
BglI GCCn_nnn'nGGC
Cuts at: 0 378 1717 2820
Size: 378 1339 1103
Fragments arranged by size:
1339 1103 378
BglII A'GATC_T
Cuts at: 0 1540 2820
Size: 1540 1280
Bme1580I G_kGCm'C
Cuts at: 0 717 2002 2820
Size: 717 1285 818
Fragments arranged by size:
1285 818 717
BmgBI CAC'GTC
Cuts at: 0 568 2820
Size: 568 2252
BmrI ACTGGGnnnn_n'
Cuts at: 0 1411 1650 2767 2820
Size: 1411 239 1117 53
Fragments arranged by size:
1411 1117 239 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2314 2820
Size: 2314 506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 1295 1934 2820
Size: 1178 117 639 886
Fragments arranged by size:
1178 886 639 117
Bpu10I CC'TnA_GC
Cuts at: 0 494 1402 2820
Size: 494 908 1418
Fragments arranged by size:
1418 908 494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 2820
Size: 280 2540
BsaI GGTCTCn'nnnn_
Cuts at: 0 1017 2285 2820
Size: 1017 1268 535
Fragments arranged by size:
1268 1017 535
BsaHI Gr'CG_yC
Cuts at: 0 1326 2820
Size: 1326 1494
BsaWI w'CCGG_w
Cuts at: 0 2207 2820
Size: 2207 613
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1275 1305 1932 1962 2820
Size: 1275 30 627 30 858
Fragments arranged by size:
1275 858 627 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 165 298 450 1559 2820
Size: 67 98 133 152 1109 1261
Fragments arranged by size:
1261 1109 152 133 98 67
BseYI C'CCAG_C
Cuts at: 0 607 2333 2820
Size: 607 1726 487
Fragments arranged by size:
1726 607 487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 1478 2593 2820
Size: 371 1107 1115 227
Fragments arranged by size:
1115 1107 371 227
BsiHKAI G_wGCw'C
Cuts at: 0 296 1728 2105 2820
Size: 296 1432 377 715
Fragments arranged by size:
1432 715 377 296
BsmI GAATG_Cn'
Cuts at: 0 351 1737 2820
Size: 351 1386 1083
Fragments arranged by size:
1386 1083 351
Bsp1286I G_dGCh'C
Cuts at: 0 296 504 590 717 930 1728 2002 2105
Size: 296 208 86 127 213 798 274 103
Cuts at: 2105 2415 2820
Size: 310 405
Fragments arranged by size:
798 405 310 296 274 213 208 127
103 86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1455 2820
Size: 1455 1365
BsrBI CCG'CTC
Cuts at: 0 159 2226 2820
Size: 159 2067 594
Fragments arranged by size:
2067 594 159
BsrDI GCAATG_nn'
Cuts at: 0 1958 2820
Size: 1958 862
BstAPI GCAn_nnn'nTGC
Cuts at: 0 721 1732 2820
Size: 721 1011 1088
Fragments arranged by size:
1088 1011 721
BstYI r'GATC_y
Cuts at: 0 8 1540 2747 2820
Size: 8 1532 1207 73
Fragments arranged by size:
1532 1207 73 8
Bsu36I CC'TnA_GG
Cuts at: 0 232 2820
Size: 232 2588
BtgI C'CryG_G
Cuts at: 0 755 1344 2286 2820
Size: 755 589 942 534
Fragments arranged by size:
942 755 589 534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2519 2820
Size: 2519 301
BtsI GCAGTG_nn'
Cuts at: 0 618 740 2820
Size: 618 122 2080
Fragments arranged by size:
2080 618 122
DraI TTT'AAA
Cuts at: 0 578 2820
Size: 578 2242
DraIII CAC_nnn'GTG
Cuts at: 0 1266 2820
Size: 1266 1554
EaeI y'GGCC_r
Cuts at: 0 2283 2820
Size: 2283 537
EarI CTCTTCn'nnn_
Cuts at: 0 589 980 1110 1805 2820
Size: 589 391 130 695 1015
Fragments arranged by size:
1015 695 589 391 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 2820
Size: 88 2732
EcoICRI GAG'CTC
Cuts at: 0 2103 2820
Size: 2103 717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1178 1265 1295 1593 1934 2380 2820
Size: 473 705 87 30 298 341 446 440
Fragments arranged by size:
705 473 446 440 341 298 87 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1582 2591 2820
Size: 1582 1009 229
Fragments arranged by size:
1582 1009 229
EcoO109I rG'GnC_Cy
Cuts at: 0 1299 2820
Size: 1299 1521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 210 242 588 620 645 677
Size: 108 32 70 32 346 32 25 32
Cuts at: 677 1275 1307 2820
Size: 598 32 1513
Fragments arranged by size:
1513 598 346 108 70 32 32 32
32 32 25
HincII GTy'rAC
Cuts at: 0 1751 2820
Size: 1751 1069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1772 1915 2820
Size: 1772 143 905
Fragments arranged by size:
1772 905 143
MscI TGG'CCA
Cuts at: 0 2285 2820
Size: 2285 535
MslI CAynn'nnrTG
Cuts at: 0 733 1885 1912 2615 2820
Size: 733 1152 27 703 205
Fragments arranged by size:
1152 733 703 205 27
MspA1I CmG'CkG
Cuts at: 0 634 2820
Size: 634 2186
NcoI C'CATG_G
Cuts at: 0 755 1344 2286 2820
Size: 755 589 942 534
Fragments arranged by size:
942 755 589 534
NsiI A_TGCA'T
Cuts at: 0 2467 2820
Size: 2467 353
NspI r_CATG'y
Cuts at: 0 1653 2820
Size: 1653 1167
PasI CC'CwG_GG
Cuts at: 0 351 2820
Size: 351 2469
PciI A'CATG_T
Cuts at: 0 1649 2820
Size: 1649 1171
PfoI T'CCnGG_A
Cuts at: 0 1078 2820
Size: 1078 1742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1932 1964 2820
Size: 1932 32 856
Fragments arranged by size:
1932 856 32
PpuMI rG'GwC_Cy
Cuts at: 0 1299 2820
Size: 1299 1521
PsiI TTA'TAA
Cuts at: 0 973 2820
Size: 973 1847
PstI C_TGCA'G
Cuts at: 0 398 2820
Size: 398 2422
PvuII CAG'CTG
Cuts at: 0 634 2820
Size: 634 2186
SacI G_AGCT'C
Cuts at: 0 2105 2820
Size: 2105 715
SanDI GG'GwC_CC
Cuts at: 0 1299 2820
Size: 1299 1521
SapI GCTCTTCn'nnn_
Cuts at: 0 980 2820
Size: 980 1840
ScaI AGT'ACT
Cuts at: 0 669 2820
Size: 669 2151
SexAI A'CCwGG_T
Cuts at: 0 1262 2539 2820
Size: 1262 1277 281
Fragments arranged by size:
1277 1262 281
SfcI C'TryA_G
Cuts at: 0 394 1205 1331 2483 2820
Size: 394 811 126 1152 337
Fragments arranged by size:
1152 811 394 337 126
SmlI C'TyrA_G
Cuts at: 0 295 861 2406 2820
Size: 295 566 1545 414
Fragments arranged by size:
1545 566 414 295
SspI AAT'ATT
Cuts at: 0 1037 2809 2820
Size: 1037 1772 11
Fragments arranged by size:
1772 1037 11
StyI C'CwwG_G
Cuts at: 0 186 755 1294 1344 2286 2307 2820
Size: 186 569 539 50 942 21 513
Fragments arranged by size:
942 569 539 513 186 50 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2347 2820
Size: 2347 473
TatI w'GTAC_w
Cuts at: 0 555 667 2820
Size: 555 112 2153
Fragments arranged by size:
2153 555 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1074 1106 1507 1539 2112 2144 2820
Size: 1074 32 401 32 573 32 676
Fragments arranged by size:
1074 676 573 401 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1153 2789 2820
Size: 1153 1636 31
Fragments arranged by size:
1636 1153 31
XmnI GAAnn'nnTTC
Cuts at: 0 1799 2736 2820
Size: 1799 937 84
Fragments arranged by size:
1799 937 84
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AhdI AleI AlfI
AloI ApoI AseI AvaI BamHI BanI BanII BbsI
BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BstAPI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII
MmeI MscI MslI MspA1I NcoI NsiI NspI PasI
PciI PfoI PpiI PpuMI PsiI PstI PvuII SacI
SanDI SapI ScaI SexAI SfcI SmlI SspI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AlwNI ApaI ApaLI
AscI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BclI BlpI BmtI BsaAI BsaBI BsiEI BsiWI BsmBI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I ClaI CspCI DrdI EagI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PflMI PmeI PmlI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI