Restriction map for
pFN21AB7818
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvrII BamHI BbeI
BbvCI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI HaeII HindIII
HpaI KasI KpnI KspI MamI
MfeI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 214 GACGTC GACGT^C
AccI 1117 GTCGAC GT^[AC][GT]AC
AccIII 250 TCCGGA T^CCGGA
AcsI 1462 GAATTC [GA]^AATT[CT]
ApoI 1462 GAATTC [GA]^AATT[CT]
AviII 830 TGCGCA TGC^GCA
BalI 470 TGGCCA TGG^CCA
BclI 1091 TGATCA T^GATCA
BfaI 563 CTAG C^TAG
BpmI 107 CTCCAG CTGGAG(16/14)
BsaWI 250 TCCGGA [AT]^CCGG[AT]
BseAI 250 TCCGGA T^CCGGA
BsmBI 719 CGTCTC CGTCTC(1/5)
BspEI 250 TCCGGA T^CCGGA
BsrFI 619 GCCGGC [GA]^CCGG[CT]
BstAPI 1410 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 619 GCCGGC [GA]^CCGG[CT]
DraI 1011 TTTAAA TTT^AAA
EcoRI 1462 GAATTC G^AATTC
FbaI 1091 TGATCA T^GATCA
FspI 830 TGCGCA TGC^GCA
HincII 1117 GTCGAC GT[CT]^[GA]AC
HindII 1117 GTCGAC GT[CT]^[GA]AC
MaeI 563 CTAG C^TAG
MluI 716 ACGCGT A^CGCGT
MluNI 470 TGGCCA TGG^CCA
MroI 250 TCCGGA T^CCGGA
MscI 470 TGGCCA TGG^CCA
MspA1I 724 CAGCTG C[AC]G^C[GT]G
NaeI 619 GCCGGC GCC^GGC
NgoMIV 619 GCCGGC G^CCGGC
PpuMI 25 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 703 CTGCAG CTGCA^G
PvuII 724 CAGCTG CAG^CTG
SalI 1117 GTCGAC G^TCGAC
SapI 1047 GAAGAGC GCTCTTC(1/4)
ScaI 947 AGTACT AGT^ACT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 214 GACGTC G[GA]^CG[CT]C
384 GACGCC
AflIII 716 ACGCGT A^C[GA][CT]GT
890 ACGTGT
AlwI 1269 GATCC GGATC(4/5)
439 GGATC
ApaI 586 GGGCCC GGGCC^C
1109 GGGCCC
Asp700I 277 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
991 GAAGTGGTTC
BbrPI 631 CACGTG CAC^GTG
1612 CACGTG
BbsI 277 GAAGAC GAAGAC(2/6)
1735 GAAGAC
BcgI 1494 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1419 GCAATGCGTTCG
BpuAI 277 GAAGAC GAAGAC(2/6)
1735 GAAGAC
BsaHI 214 GACGTC G[GA]^CG[CT]C
384 GACGCC
BsgI 592 CTGCAC GTGCAG(16/14)
409 GTGCAG
BstF5I 1248 CATCC GGATG(2/0)
980 GGATG
BstYI 439 GGATCT [GA]^GATC[CT]
1268 AGATCC
DraIII 628 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
631 CACGTGGTG
DsaI 686 CCATGG C^C[GA][CT]GG
1238 CCATGG
EaeI 470 TGGCCA [CT]^GGCC[GA]
711 CGGCCA
EcoT14I 686 CCATGG C^C[AT][AT]GG
1238 CCATGG
FokI 1248 CATCC GGATG(9/13)
980 GGATG
Hin1I 214 GACGTC G[GA]^CG[CT]C
384 GACGCC
MflI 439 GGATCT [GA]^GATC[CT]
1268 AGATCC
NcoI 686 CCATGG C^CATGG
1238 CCATGG
PflMI 627 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
630 CCACGTGGTGG
PleI 1120 GACTC GAGTC(4/5)
1086 GAGTC
PmaCI 631 CACGTG CAC^GTG
1612 CACGTG
PmlI 631 CACGTG CAC^GTG
1612 CACGTG
PshAI 254 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
922 GACAAGCGTC
SmlI 35 CTCAAG C^T[CT][GA]AG
1552 CTCAAG
StyI 686 CCATGG C^C[AT][AT]GG
1238 CCATGG
Tsp45I 1534 GTCAC ^GT[GC]AC
1615 GTGAC
Van91I 627 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
630 CCACGTGGTGG
XhoII 439 GGATCT [GA]^GATC[CT]
1268 AGATCC
XmnI 277 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
991 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XmaCI XmaI