(Circular) (Six-base) MAPSORT of: pFN21AB7818.seq Check: 7979 from: 1 to: 6499 pFN21AB7818 6499 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2328 2734 2328 Size: 406 6093 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2223 4309 278 Size: 53 83 186 936 687 2086 2468 Fragments arranged by size: 2468 2086 936 687 186 83 53 AccI GT'mk_AC Cuts at: 1937 3123 3842 1937 Size: 1186 719 4594 Fragments arranged by size: 4594 1186 719 Acc65I G'GTAC_C Cuts at: 3820 3820 Size: 6499 AclI AA'CG_TT Cuts at: 1563 4626 4999 1563 Size: 3063 373 3063 Fragments arranged by size: 3063 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2167 4562 5946 6214 1632 Size: 535 2395 1384 268 1917 Fragments arranged by size: 2395 1917 1384 535 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6302 AflIII A'CryG_T Cuts at: 1203 2721 2895 5398 6375 1203 Size: 1518 174 2503 977 1327 Fragments arranged by size: 2503 1518 1327 977 174 AgeI A'CCGG_T Cuts at: 5330 5330 Size: 6499 AhdI GACnn_n'nnGTC Cuts at: 5228 5228 Size: 6499 AleI CACnn'nnGTG Cuts at: 1166 2561 2744 1166 Size: 1395 183 4921 Fragments arranged by size: 4921 1395 183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2901 1184 Size: 1717 4782 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3330 3362 3330 Size: 32 6467 AlwNI CAG_nnn'CTG Cuts at: 4242 5814 4242 Size: 1572 4927 ApaI G_GGCC'C Cuts at: 2595 3118 2595 Size: 523 5976 ApaLI G'TGCA_C Cuts at: 4556 5712 4556 Size: 1156 5343 ApoI r'AATT_y Cuts at: 1493 3467 3808 4034 4186 6295 1493 Size: 1974 341 226 152 2109 1697 Fragments arranged by size: 2109 1974 1697 341 226 152 AseI AT'TA_AT Cuts at: 160 1032 5053 160 Size: 872 4021 1606 Fragments arranged by size: 4021 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 AvaI C'yCGr_G Cuts at: 1870 1943 2401 2440 2701 3824 1870 Size: 73 458 39 261 1123 4545 Fragments arranged by size: 4545 1123 458 261 73 39 BamHI G'GATC_C Cuts at: 3829 4196 3829 Size: 367 6132 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2555 2746 3748 3820 5275 Size: 325 217 627 768 191 1002 72 1455 Cuts at: 5275 618 Size: 1842 Fragments arranged by size: 1842 1455 1002 768 627 325 217 191 72 BanII G_rGCy'C Cuts at: 1391 2595 2622 2771 3059 3069 3118 3558 3818 Size: 1204 27 149 288 10 49 440 260 Cuts at: 3818 1391 Size: 4072 Fragments arranged by size: 4072 1204 440 288 260 149 49 27 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2289 3747 928 Size: 971 390 1458 3680 Fragments arranged by size: 3680 1458 971 390 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3413 3447 3488 3522 4690 Size: 34 50 34 1392 34 41 34 1168 Cuts at: 4690 4724 1903 Size: 34 3678 Fragments arranged by size: 3678 1392 1168 50 41 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4391 5601 4391 Size: 1210 5289 BclI T'GATC_A Cuts at: 1548 3096 1548 Size: 1548 4951 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5110 136 Size: 107 122 71 631 605 3438 1525 Fragments arranged by size: 3438 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6494 6494 Size: 6499 BlpI GC'TnA_GC Cuts at: 3916 3916 Size: 6499 Bme1580I G_kGCm'C Cuts at: 1790 2595 2667 2751 3106 3118 4560 5716 1790 Size: 805 72 84 355 12 1442 1156 2573 Fragments arranged by size: 2573 1442 1156 805 355 84 72 12 BmgBI CAC'GTC Cuts at: 1318 2602 1318 Size: 1284 5215 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2323 2915 3368 Size: 416 53 83 399 24 906 592 453 Cuts at: 3368 4230 5188 442 Size: 862 958 1753 Fragments arranged by size: 1753 958 906 862 592 453 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6499 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2097 5159 1239 Size: 858 3062 2579 Fragments arranged by size: 3062 2579 858 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2025 3542 4624 5489 5787 2025 Size: 1517 1082 865 298 2737 Fragments arranged by size: 2737 1517 1082 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5162 882 Size: 617 3663 2219 Fragments arranged by size: 3663 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2473 2638 2896 3619 4290 493 Size: 711 1269 165 258 723 671 2702 Fragments arranged by size: 2702 1269 723 711 671 258 165 BsaBI GATnn'nnATC Cuts at: 6493 6493 Size: 6499 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2220 2390 4306 4688 Size: 53 83 186 936 687 170 1916 382 Cuts at: 4688 5323 275 Size: 635 1451 Fragments arranged by size: 1916 1451 936 687 635 382 186 170 83 53 BsaWI w'CCGG_w Cuts at: 2255 4932 5330 5604 5751 6162 2255 Size: 2677 398 274 147 411 2592 Fragments arranged by size: 2677 2592 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2360 2390 3019 3049 1178 Size: 30 165 30 957 30 629 30 4628 Fragments arranged by size: 4628 957 629 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2368 3463 1186 Size: 1165 17 1095 4222 Fragments arranged by size: 4222 1165 1095 17 BseYI C'CCAG_C Cuts at: 2177 3204 3261 5702 6198 2177 Size: 1027 57 2441 496 2478 Fragments arranged by size: 2478 2441 1027 496 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 2582 1163 Size: 573 699 147 5080 Fragments arranged by size: 5080 699 573 147 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3949 4710 5738 1540 Size: 275 186 1948 761 1028 2301 Fragments arranged by size: 2301 1948 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4560 4645 5716 4560 Size: 85 1071 5343 Fragments arranged by size: 5343 1071 85 BsmI GAATG_Cn' Cuts at: 4010 4103 4010 Size: 93 6406 BsmBI CGTCTCn'nnnn_ Cuts at: 2730 2730 Size: 6499 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2595 2622 2667 2751 2771 3059 3069 Size: 399 805 27 45 84 20 288 10 Cuts at: 3069 3106 3118 3558 3818 4560 4645 5716 1391 Size: 37 12 440 260 742 85 1071 2174 Fragments arranged by size: 2174 1071 805 742 440 399 288 260 85 84 45 37 27 20 12 10 BspEI T'CCGG_A Cuts at: 2255 6162 2255 Size: 3907 2592 BspHI T'CATG_A Cuts at: 4388 6118 4388 Size: 1730 4769 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 2734 3300 3321 3854 844 Size: 1484 406 566 21 533 3489 Fragments arranged by size: 3489 1484 566 533 406 21 BsrBI CCG'CTC Cuts at: 1394 1823 4386 1394 Size: 429 2563 3507 Fragments arranged by size: 3507 2563 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3406 3431 3519 4994 5168 65 Size: 1183 2158 25 88 1475 174 1396 Fragments arranged by size: 2158 1475 1396 1183 174 88 25 BsrFI r'CCGG_y Cuts at: 1700 1810 2624 5143 5330 1700 Size: 110 814 2519 187 2869 Fragments arranged by size: 2869 2519 814 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6499 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6499 BssSI C'ACGA_G Cuts at: 4559 5571 4559 Size: 1012 5487 BstAPI GCAn_nnn'nTGC Cuts at: 794 3421 794 Size: 2627 3872 BstBI TT'CG_AA Cuts at: 5303 5303 Size: 6499 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6499 BstXI CCAn_nnnn'nTGG Cuts at: 3376 3574 3670 3376 Size: 198 96 6205 Fragments arranged by size: 6205 198 96 BstYI r'GATC_y Cuts at: 1972 2444 3273 3829 4196 4581 4598 6050 6136 Size: 472 829 556 367 385 17 1452 86 Cuts at: 6136 6148 6494 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 829 556 472 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2691 3243 513 Size: 552 1626 552 3769 Fragments arranged by size: 3769 1626 552 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5760 Fragments arranged by size: 5760 683 56 BtsI GCAGTG_nn' Cuts at: 2157 4015 4809 4829 2157 Size: 1858 794 20 3827 Fragments arranged by size: 3827 1858 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6499 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2217 2252 529 Size: 35 1653 35 4776 Fragments arranged by size: 4776 1653 35 35 DraI TTT'AAA Cuts at: 3018 3803 3018 Size: 785 5714 DraIII CAC_nnn'GTG Cuts at: 2638 2641 2638 Size: 3 6496 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5506 809 Size: 507 4190 1802 Fragments arranged by size: 4190 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2475 2716 3946 Size: 54 1425 50 275 18 645 241 1230 Cuts at: 3946 4835 6464 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1230 889 645 275 241 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3946 1537 Size: 275 2134 4090 Fragments arranged by size: 4090 2134 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2086 2534 3047 4429 1353 Size: 733 448 513 1382 3423 Fragments arranged by size: 3423 1382 733 513 448 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5086 5460 5606 387 Size: 1064 138 3497 374 146 1280 Fragments arranged by size: 3497 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2167 4562 5159 5946 6214 1239 Size: 393 465 70 2395 597 787 268 1524 Fragments arranged by size: 2395 1524 787 597 465 393 268 70 EcoO109I rG'GnC_Cy Cuts at: 2031 2592 3114 2031 Size: 561 522 5416 Fragments arranged by size: 5416 561 522 EcoRI G'AATT_C Cuts at: 3467 3808 4186 3467 Size: 341 378 5780 Fragments arranged by size: 5780 378 341 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3332 3332 Size: 6499 FspI TGC'GCA Cuts at: 2837 5005 2837 Size: 2168 4331 FspAI rTGC'GCAy Cuts at: 2837 2837 Size: 6499 HaeII r_GCGC'y Cuts at: 1678 5646 1678 Size: 3968 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 3540 3572 5140 Size: 32 135 32 857 32 1079 32 1568 Cuts at: 5140 5172 5214 5246 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1568 1079 857 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3124 3843 4089 669 Size: 988 281 1186 719 246 3079 Fragments arranged by size: 3079 1186 988 719 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6499 HpaI GTT'AAC Cuts at: 4089 4089 Size: 6499 KpnI G_GTAC'C Cuts at: 3824 3824 Size: 6499 MfeI C'AATT_G Cuts at: 4098 4098 Size: 6499 MluI A'CGCG_T Cuts at: 2721 6375 2721 Size: 3654 2845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2802 3153 3393 3501 5613 5797 1305 Size: 1497 351 240 108 2112 184 2007 Fragments arranged by size: 2112 2007 1497 351 240 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 2477 10 Size: 54 1425 343 645 4032 Fragments arranged by size: 4032 1425 645 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 2561 2744 4457 4816 4975 518 Size: 648 1125 270 183 1713 359 159 2042 Fragments arranged by size: 2042 1713 1125 648 359 270 183 159 MspA1I CmG'CkG Cuts at: 1606 2731 3915 4592 5740 5985 1606 Size: 1125 1184 677 1148 245 2120 Fragments arranged by size: 2120 1184 1148 1125 677 245 NaeI GCC'GGC Cuts at: 2626 2626 Size: 6499 NcoI C'CATG_G Cuts at: 513 1065 2691 3243 513 Size: 552 1626 552 3769 Fragments arranged by size: 3769 1626 552 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6499 NgoMIV G'CCGG_C Cuts at: 2624 2624 Size: 6499 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6499 NotI GC'GGCC_GC Cuts at: 3946 3946 Size: 6499 NspI r_CATG'y Cuts at: 1132 1229 3857 5402 1132 Size: 97 2628 1545 2229 Fragments arranged by size: 2628 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6499 PciI A'CATG_T Cuts at: 5398 5398 Size: 6499 PflMI CCAn_nnn'nTGG Cuts at: 1108 2638 2641 1108 Size: 1530 3 4966 Fragments arranged by size: 4966 1530 3 PfoI T'CCnGG_A Cuts at: 3780 3780 Size: 6499 PmeI GTTT'AAAC Cuts at: 3803 3803 Size: 6499 PmlI CAC'GTG Cuts at: 2638 3619 2638 Size: 981 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4568 4600 6081 6113 4568 Size: 32 1481 32 4954 Fragments arranged by size: 4954 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2031 2031 Size: 6499 PshAI GACnn'nnGTC Cuts at: 1613 2263 2931 1613 Size: 650 668 5181 Fragments arranged by size: 5181 668 650 PsiI TTA'TAA Cuts at: 4069 4069 Size: 6499 PspOMI G'GGCC_C Cuts at: 2591 3114 2591 Size: 523 5976 PstI C_TGCA'G Cuts at: 830 2712 3851 830 Size: 1882 1139 3478 Fragments arranged by size: 3478 1882 1139 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6499 PvuII CAG'CTG Cuts at: 2731 2731 Size: 6499 SalI G'TCGA_C Cuts at: 1936 3122 3841 1936 Size: 1186 719 4594 Fragments arranged by size: 4594 1186 719 SapI GCTCTTCn'nnn_ Cuts at: 3047 3047 Size: 6499 SbfI CC_TGCA'GG Cuts at: 3851 3851 Size: 6499 ScaI AGT'ACT Cuts at: 2954 4747 6459 2954 Size: 1793 1712 2994 Fragments arranged by size: 2994 1793 1712 SfcI C'TryA_G Cuts at: 826 1045 2068 2203 2708 3713 3847 4982 5663 Size: 219 1023 135 505 1005 134 1135 681 Cuts at: 5663 5854 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1023 1005 681 505 219 191 135 134 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6499 SmaI CCC'GGG Cuts at: 1872 2403 2442 2703 3826 1872 Size: 531 39 261 1123 4545 Fragments arranged by size: 4545 1123 531 261 39 SmlI C'TyrA_G Cuts at: 820 1017 1943 2040 3557 4603 5504 5766 820 Size: 197 926 97 1517 1046 901 262 1553 Fragments arranged by size: 1553 1517 1046 926 901 262 197 97 SnaBI TAC'GTA Cuts at: 493 4290 493 Size: 3797 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6499 SphI G_CATG'C Cuts at: 1132 3857 1132 Size: 2725 3774 SspI AAT'ATT Cuts at: 5 52 4423 5 Size: 47 4371 2081 Fragments arranged by size: 4371 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6499 StyI C'CwwG_G Cuts at: 513 1065 2691 3243 3938 513 Size: 552 1626 552 695 3074 Fragments arranged by size: 3074 1626 695 552 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3218 4526 4543 4696 4881 5326 325 Size: 1255 1638 1308 17 153 185 445 1498 Fragments arranged by size: 1638 1498 1308 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2952 4745 6457 Size: 274 80 33 51 1443 975 1793 1712 Cuts at: 6457 96 Size: 138 Fragments arranged by size: 1793 1712 1443 975 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6459 6491 6459 Size: 32 6467 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6499 XbaI T'CTAG_A Cuts at: 3835 3835 Size: 6499 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6499 XmaI C'CCGG_G Cuts at: 1870 2401 2440 2701 3824 1870 Size: 531 39 261 1123 4545 Fragments arranged by size: 4545 1123 531 261 39 XmnI GAAnn'nnTTC Cuts at: 2286 3000 4628 2286 Size: 714 1628 4157 Fragments arranged by size: 4157 1628 714 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2221 4307 276 Size: 53 83 186 936 687 2086 2468 Fragments arranged by size: 2468 2086 936 687 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI