################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI MwoI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 409 GGCGTC G[GA]^CG[CT]C ApaI 298 GGGCCC GGGCC^C BamHI 741 GGATCC G^GATCC BanII 298 GGGCCC G[GA]GC[CT]^C BbsI 37 GAAGAC GAAGAC(2/6) BclI 2 TGATCA T^GATCA BfaI 211 CTAG C^TAG BmyI 298 GGGCCC G[AGT]GC[ACT]^C BpuAI 37 GAAGAC GAAGAC(2/6) BsaHI 409 GGCGTC G[GA]^CG[CT]C BsiYI 301 CCCAACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 301 CCCAACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 347 GTCTC GTCTC(1/5) BsmBI 346 CGTCTC CGTCTC(1/5) Bsp1286I 298 GGGCCC G[AGT]GC[ACT]^C BspHI 671 TCATGA T^CATGA BsrDI 736 GCAATG GCAATG(2/0) BsrFI 103 GCCGGT [GA]^CCGG[CT] Cfr10I 103 GCCGGT [GA]^CCGG[CT] DraI 436 TTTAAA TTT^AAA DraII 297 AGGGCCC [GA]G^G[ACGT]CC[CT] DsaI 606 CCATGG C^C[GA][CT]GG EaeI 186 CGGCCA [CT]^GGCC[GA] EcoO109I 297 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoT14I 606 CCATGG C^C[AT][AT]GG FbaI 2 TGATCA T^GATCA HapII 104 CCGG C^CGG HgaI 410 GCGTC GACGC(5/10) Hin1I 409 GGCGTC G[GA]^CG[CT]C HpaII 104 CCGG C^CGG MaeI 211 CTAG C^TAG MslI 238 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 707 CAGCTG C[AC]G^C[GT]G MspI 104 CCGG C^CGG NcoI 606 CCATGG C^CATGG NdeI 84 CATATG CA^TATG PleI 497 GACTC GAGTC(4/5) PvuII 707 CAGCTG CAG^CTG RcaI 671 TCATGA T^CATGA ScaI 778 AGTACT AGT^ACT StyI 606 CCATGG C^C[AT][AT]GG Tsp45I 703 GTGAC ^GT[GC]AC TspRI 701 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 166 GCGG CCGC(-3/-1) 185 GCGG BciVI 277 GGATAC GTATCC(6/5) 748 GTATCC BpmI 443 CTCCAG CTGGAG(16/14) 710 CTGGAG BstYI 420 AGATCC [GA]^GATC[CT] 741 GGATCC Cfr13I 298 GGGCC G^G[ACGT]CC 299 GGCCC HaeIII 187 GGCC GG^CC 299 GGCC HphI 146 TCACC GGTGA(8/7) 519 TCACC MaeIII 593 GTTAC ^GT[ACGT]AC 703 GTGAC MflI 420 AGATCC [GA]^GATC[CT] 741 GGATCC MnlI 214 GAGG CCTC(7/6) 713 GAGG MseI 10 TTAA T^TAA 437 TTAA NlaIV 298 GGGCCC GG[ACGT]^[ACGT]CC 741 GGATCC Sau96I 298 GGGCC G^G[ACGT]CC 299 GGCCC SfaNI 370 GATGC GCATC(5/9) 694 GATGC SmlI 44 CTCAAG C^T[CT][GA]AG 499 CTCAAG TfiI 56 GATTC G^A[AT]TC 341 GAATC Tru9I 10 TTAA T^TAA 437 TTAA XhoII 420 AGATCC [GA]^GATC[CT] 741 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BsaJI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HinfI ItaI MboI MboII MvaI NdeII NlaIII RsaI Sau3AI ScrFI TseI Tsp509I