Restriction map for
pFN21AE6670
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KspI MaeII MamI MfeI MflI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 811 GGTACC G^GTACC
AfaI 812 GTAC GT^AC
AflIII 454 ACATGT A^C[GA][CT]GT
AlwI 103 GGATC GGATC(4/5)
Asp718I 811 GGTACC G^GTACC
AspHI 595 GTGCTC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BanI 811 GGTACC G^G[CT][GA]CC
BbvCI 649 GCTGAGG CCTCAGC(-5/-2)
BfaI 307 CTAG C^TAG
BmyI 595 GTGCTC G[AGT]GC[ACT]^C
BpmI 143 CTCCAG CTGGAG(16/14)
BsiHKAI 595 GTGCTC G[AT]GC[AT]^C
BsiYI 679 CCATCATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 679 CCATCATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 595 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 454 ACATGT A^CATGT
BsrI 30 ACTGG ACTGG(1/-1)
BstXI 216 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 186 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 641 CTCTTC CTCTTC(1/4)
EcoO109I 186 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 774 CCTTGG C^C[AT][AT]GG
HphI 246 TCACC GGTGA(8/7)
KpnI 811 GGTACC GGTAC^C
Ksp632I 641 CTCTTC CTCTTC(1/4)
MaeI 307 CTAG C^TAG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 247 CACCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 640 GCTCTTCGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 454 ACATGT [GA]CATG^[CT]
PflMI 679 CCATCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 121 GAGTC GAGTC(4/5)
PpuMI 186 GGGACCC [GA]G^G[AT]CC[CT]
PstI 98 CTGCAG CTGCA^G
RsaI 812 GTAC GT^AC
SapI 640 GCTCTTC GCTCTTC(1/4)
SfcI 98 CTGCAG C^T[GA][CT]AG
SmlI 200 CTCAAG C^T[CT][GA]AG
StyI 774 CCTTGG C^C[AT][AT]GG
Tsp45I 245 GTCAC ^GT[GC]AC
Van91I 679 CCATCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 364 GAATTT [GA]^AATT[CT]
629 AAATTT
ApoI 364 GAATTT [GA]^AATT[CT]
629 AAATTT
BsgI 137 CTGCAC GTGCAG(16/14)
407 GTGCAG
BsmAI 123 GTCTC GTCTC(1/5)
803 GTCTC
BsmFI 186 GGGAC GGGAC(10/14)
256 GGGAC
BstF5I 117 GGATG GGATG(2/0)
778 GGATG
DpnII 104 GATC ^GATC
205 GATC
FokI 117 GGATG GGATG(9/13)
778 GGATG
HaeIII 3 GGCC GG^CC
170 GGCC
MboI 104 GATC ^GATC
205 GATC
NdeII 104 GATC ^GATC
205 GATC
Sau3AI 104 GATC ^GATC
205 GATC
SfaNI 379 GATGC GCATC(5/9)
779 GATGC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI AvaII BbvI BsaJI BssKI
BstNI Cfr13I DdeI EcoRII Fnu4HI
HinfI ItaI MaeIII MboII MnlI
MseI MvaI NlaIII NlaIV Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I