(Circular) (Six-base) MAPSORT of: pFN21AE4105.seq Check: 9842 from: 1 to: 6130 pFN21AE4105 6130 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2312 3363 3473 1937 Size: 375 1051 110 4594 Fragments arranged by size: 4594 1051 375 110 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3089 4193 5577 5845 1632 Size: 1457 1104 1384 268 1917 Fragments arranged by size: 1917 1457 1384 1104 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 5029 6006 1203 Size: 3826 977 1327 Fragments arranged by size: 3826 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2663 2742 1184 Size: 1479 79 4572 Fragments arranged by size: 4572 1479 79 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3263 3295 3263 Size: 32 6098 AlwNI CAG_nnn'CTG Cuts at: 2072 2377 3873 5445 2072 Size: 305 1496 1572 2757 Fragments arranged by size: 2757 1572 1496 305 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 2020 2047 2348 2835 2875 3019 3439 3665 Size: 527 27 301 487 40 144 420 226 Cuts at: 3665 3817 5926 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 527 487 420 301 226 152 144 40 27 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 3455 1870 Size: 73 1512 4545 Fragments arranged by size: 4545 1512 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2019 2052 2472 2505 2019 Size: 33 420 33 5644 Fragments arranged by size: 5644 420 33 33 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3451 4906 618 Size: 325 217 627 1664 1455 1842 Fragments arranged by size: 1842 1664 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3387 3449 1391 Size: 1996 62 4072 Fragments arranged by size: 4072 1996 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2794 3432 928 Size: 971 895 638 3626 Fragments arranged by size: 3626 971 895 638 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4321 4355 1903 Size: 34 50 34 2300 34 3678 Fragments arranged by size: 3678 2300 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BfrBI ATG'CAT Cuts at: 2907 2907 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3408 6125 3408 Size: 2717 3413 BlpI GC'TnA_GC Cuts at: 2117 3381 3547 2117 Size: 1264 166 4700 Fragments arranged by size: 4700 1264 166 Bme1580I G_kGCm'C Cuts at: 1790 2400 4191 5347 1790 Size: 610 1791 1156 2573 Fragments arranged by size: 2573 1791 1156 610 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2403 3861 4819 Size: 416 53 83 399 24 986 1458 958 Cuts at: 4819 442 Size: 1753 Fragments arranged by size: 1753 1458 986 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4790 1239 Size: 3551 2579 Bpu10I CC'TnA_GC Cuts at: 3093 3093 Size: 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4255 5120 5418 4255 Size: 865 298 4967 Fragments arranged by size: 4967 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3059 4563 4961 5235 5382 5793 3059 Size: 1504 398 274 147 411 3396 Fragments arranged by size: 3396 1504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3180 3210 1178 Size: 30 165 30 1777 30 4098 Fragments arranged by size: 4098 1777 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6130 BseYI C'CCAG_C Cuts at: 5333 5829 5333 Size: 496 5634 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2837 1163 Size: 573 1101 4456 Fragments arranged by size: 4456 1101 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2431 2694 3067 3387 4191 4276 5347 2431 Size: 263 373 320 804 85 1071 3214 Fragments arranged by size: 3214 1071 804 373 320 263 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2400 2431 2694 3067 3387 3449 4191 Size: 399 610 31 263 373 320 62 742 Cuts at: 4191 4276 5347 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 610 399 373 320 263 85 62 31 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3100 4017 1394 Size: 429 1277 917 3507 Fragments arranged by size: 3507 1277 917 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2909 4625 4799 65 Size: 1183 1661 1716 174 1396 Fragments arranged by size: 1716 1661 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4774 4961 1700 Size: 110 2964 187 2869 Fragments arranged by size: 2964 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3187 96 Size: 3091 3039 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstYI r'GATC_y Cuts at: 1972 2630 3408 3460 3827 4212 4229 5681 5767 Size: 658 778 52 367 385 17 1452 86 Cuts at: 5767 5779 6125 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 778 658 385 367 346 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2034 2888 513 Size: 552 969 854 3755 Fragments arranged by size: 3755 969 854 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 3646 4440 4460 3646 Size: 794 20 5316 Fragments arranged by size: 5316 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DraIII CAC_nnn'GTG Cuts at: 2860 2860 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3255 3577 4466 Size: 54 1425 50 275 18 1425 322 889 Cuts at: 4466 6095 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1425 889 322 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3003 3107 4060 1353 Size: 1650 104 953 3423 Fragments arranged by size: 3423 1650 953 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3192 4717 5091 5237 387 Size: 1064 138 1603 1525 374 146 1280 Fragments arranged by size: 1603 1525 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3385 3385 Size: 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3089 4193 4790 5577 5845 1239 Size: 393 1457 1104 597 787 268 1524 Fragments arranged by size: 1524 1457 1104 787 597 393 268 EcoRI G'AATT_C Cuts at: 2047 3439 3817 2047 Size: 1392 378 4360 Fragments arranged by size: 4360 1392 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2488 2488 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 5277 1678 Size: 3599 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2982 3014 4771 4803 4845 Size: 32 135 32 1410 32 1757 32 42 Cuts at: 4845 4877 1373 Size: 32 2626 Fragments arranged by size: 2626 1757 1410 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3001 3474 3720 669 Size: 988 281 1063 473 246 3079 Fragments arranged by size: 3079 1063 988 473 281 246 HindIII A'AGCT_T Cuts at: 748 3289 748 Size: 2541 3589 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 2565 3729 2565 Size: 1164 4966 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5244 5428 1305 Size: 3939 184 2007 Fragments arranged by size: 3939 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3257 10 Size: 54 1425 343 1425 2883 Fragments arranged by size: 2883 1425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2724 4088 4447 4606 518 Size: 648 1558 1364 359 159 2042 Fragments arranged by size: 2042 1558 1364 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3546 4223 5371 5616 1606 Size: 1940 677 1148 245 2120 Fragments arranged by size: 2120 1940 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5578 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NsiI A_TGCA'T Cuts at: 2909 2909 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 3488 5033 1132 Size: 97 2259 1545 2229 Fragments arranged by size: 2259 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6130 PfoI T'CCnGG_A Cuts at: 2318 3081 2318 Size: 763 5367 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 3482 830 Size: 2652 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 SacI G_AGCT'C Cuts at: 3387 3387 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SapI GCTCTTCn'nnn_ Cuts at: 3107 3107 Size: 6130 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2510 3478 4613 5294 5485 826 Size: 219 1465 968 1135 681 191 1471 Fragments arranged by size: 1471 1465 1135 968 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4234 5135 5397 820 Size: 197 926 2291 901 262 1553 Fragments arranged by size: 2291 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2059 2445 3569 513 Size: 552 994 386 1124 3074 Fragments arranged by size: 3074 1124 994 552 386 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4157 4174 4327 4512 4957 325 Size: 1255 2577 17 153 185 445 1498 Fragments arranged by size: 2577 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3187 3399 4376 Size: 274 80 33 51 1443 1210 212 977 Cuts at: 4376 6088 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1210 977 274 212 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2714 2746 6090 6122 2714 Size: 32 3344 32 2722 Fragments arranged by size: 3344 2722 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 2932 3466 2932 Size: 534 5596 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3289 3289 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 2791 4259 2791 Size: 1468 4662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI