################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BcgI BciVI BcnI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRII EcoT22I FauI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeII MamI MluI MluNI MroI MscI MspA1I MvaI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI TaqI TfiI Tth111I TthHB8I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflII 594 CTTAAG C^TTAAG AlwNI 154 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG BanI 859 GGTGCC G^G[CT][GA]CC BbvCI 114 GCTGAGG CCTCAGC(-5/-2) BclI 501 TGATCA T^GATCA BfrI 594 CTTAAG C^TTAAG BpmI 164 CTCCAG CTGGAG(16/14) BsaJI 322 CCTTGG C^C[ACGT][ACGT]GG BsgI 37 GTGCAG GTGCAG(16/14) BsmAI 635 GAGAC GTCTC(1/5) BsmI 402 GAATGC GAATGC(1/-1) BspMI 897 ACCTGC ACCTGC(4/8) BsrDI 45 CATTGC GCAATG(2/0) BsrFI 24 GCCGGC [GA]^CCGG[CT] BstYI 156 GGATCT [GA]^GATC[CT] CfoI 42 GCGC GCG^C Cfr10I 24 GCCGGC [GA]^CCGG[CT] Cfr13I 281 GGGCC G^G[ACGT]CC DdeI 115 CTGAG C^T[ACGT]AG DraI 831 TTTAAA TTT^AAA EcoRV 607 GATATC GAT^ATC EcoT14I 322 CCTTGG C^C[AT][AT]GG FbaI 501 TGATCA T^GATCA HapII 25 CCGG C^CGG HgaI 318 GCGTC GACGC(5/10) HhaI 42 GCGC GCG^C HinP1I 42 GCGC G^CGC HpaII 25 CCGG C^CGG MaeIII 519 GTCAC ^GT[ACGT]AC MfeI 641 CAATTG C^AATTG MflI 156 GGATCT [GA]^GATC[CT] MslI 94 CACCTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 25 CCGG C^CGG MunI 641 CAATTG C^AATTG NaeI 24 GCCGGC GCC^GGC NgoMIV 24 GCCGGC G^CCGGC Sau96I 281 GGGCC G^G[ACGT]CC SphI 879 GCATGC GCATG^C SspI 694 AATATT AAT^ATT StuI 351 AGGCCT AGG^CCT StyI 322 CCTTGG C^C[AT][AT]GG SwaI 830 ATTTAAAT ATTT^AAAT Tsp45I 519 GTCAC ^GT[GC]AC XbaI 477 TCTAGA T^CTAGA XcmI 203 CCATCTATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 156 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 49 GCGG CCGC(-3/-1) 561 GCGG AlwI 651 GATCC GGATC(4/5) 156 GGATC BspHI 303 TCATGA T^CATGA 870 TCATGA BstF5I 75 GGATG GGATG(2/0) 563 GGATG EcoNI 96 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 354 CCTTATGAAGG FokI 75 GGATG GGATG(9/13) 563 GGATG HinfI 517 GAGTC G^A[ACGT]TC 637 GACTC MwoI 283 GCCAAAATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 715 GCTGGACAAGC NspI 756 ACATGC [GA]CATG^[CT] 879 GCATGC PleI 637 GACTC GAGTC(4/5) 517 GAGTC RcaI 303 TCATGA T^CATGA 870 TCATGA SmlI 16 CTTGAG C^T[CT][GA]AG 594 CTTAAG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AcsI AluI ApoI BbvI BfaI BsiYI BslI BsrI Cac8I DpnII Fnu4HI HaeIII ItaI MaeI MboI MboII MnlI MseI NdeII NlaIII NlaIV Sau3AI Tru9I TseI Tsp509I TspRI