################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AviII AvrII BbrPI BbsI BfaI BfrI BglI BlnI BpuAI BsaBI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoT22I FauI FseI FspI HpaI KpnI KspI MaeI MamI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 139 CGCG CG^CG AcyI 2143 GGCGCC G[GA]^CG[CT]C ApaI 1560 GGGCCC GGGCC^C Asp700I 889 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1933 CCCGGG C^[CT]CG[GA]G BbeI 2143 GGCGCC GGCGC^C BcgI 1003 CGATCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1484 GTATCC GTATCC(6/5) BclI 630 TGATCA T^GATCA BglII 1388 AGATCT A^GATCT BsaHI 2143 GGCGCC G[GA]^CG[CT]C BsoBI 1933 CCCGGG C^[CT]CG[GA]G BstUI 139 CGCG CG^CG BstXI 1720 CCACATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 1782 TTTAAA TTT^AAA EcoRI 700 GAATTC G^AATTC FbaI 630 TGATCA T^GATCA HgaI 739 GACGC GACGC(5/10) Hin1I 2143 GGCGCC G[GA]^CG[CT]C HincII 1735 GTTGAC GT[CT]^[GA]AC HindII 1735 GTTGAC GT[CT]^[GA]AC HindIII 1921 AAGCTT A^AGCTT KasI 2143 GGCGCC G^GCGCC MvnI 139 CGCG CG^CG NarI 2143 GGCGCC GG^CGCC NdeI 1811 CATATG CA^TATG PflMI 214 CCATACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1499 GGGACCT [GA]G^G[AT]CC[CT] PstI 1504 CTGCAG CTGCA^G SapI 2127 GCTCTTC GCTCTTC(1/4) SfoI 2143 GGCGCC GGC^GCC SmaI 1933 CCCGGG CCC^GGG Van91I 214 CCATACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2060 CCAAGGACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1933 CCCGGG C^CCGGG XmaI 1933 CCCGGG C^CCGGG XmnI 889 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 494 ACATGT A^C[GA][CT]GT 1722 ACATGT AseI 394 ATTAAT AT^TAAT 1318 ATTAAT AsnI 394 ATTAAT AT^TAAT 1318 ATTAAT BalI 1894 TGGCCA TGG^CCA 2057 TGGCCA BlpI 964 GCTGAGC GC^T[ACGT]AGC 1927 GCTGAGC Bpu1102I 964 GCTGAGC GC^T[ACGT]AGC 1927 GCTGAGC BsaAI 1468 TACGTG [CT]AC^GT[GA] 1821 TACGTG BsaWI 334 ACCGGA [AT]^CCGG[AT] 704 TCCGGT BsgI 88 CTGCAC GTGCAG(16/14) 2138 GTGCAG BspHI 227 TCATGA T^CATGA 980 TCATGA BspLU11I 494 ACATGT A^CATGT 1722 ACATGT BstAPI 1165 GCAAATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2210 GCAAAGACTGC BstEII 2034 GGTGACC G^GT[ACGT]ACC 2116 GGTGACC BstPI 2034 GGTGACC G^GT[ACGT]ACC 2116 GGTGACC CelII 964 GCTGAGC GC^T[ACGT]AGC 1927 GCTGAGC DraII 1499 GGGACCT [GA]G^G[ACGT]CC[CT] 1559 AGGGCCC DraIII 12 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG 2101 CACTGTGTG EarI 7 CTCTTC CTCTTC(1/4) 2128 CTCTTC EcoO109I 1499 GGGACCT [GA]G^G[ACGT]CC[CT] 1559 AGGGCCC EcoO65I 2034 GGTGACC G^GT[ACGT]ACC 2116 GGTGACC EcoRV 406 GATATC GAT^ATC 1306 GATATC HaeII 1906 AGCGCC [GA]GCGC^[CT] 2143 GGCGCC Ksp632I 7 CTCTTC CTCTTC(1/4) 2128 CTCTTC MaeII 1469 ACGT A^CGT 1822 ACGT MluNI 1894 TGGCCA TGG^CCA 2057 TGGCCA MscI 1894 TGGCCA TGG^CCA 2057 TGGCCA MspA1I 362 CAGCTG C[AC]G^C[GT]G 2133 CAGCTG NcoI 1479 CCATGG C^CATGG 1878 CCATGG PshBI 394 ATTAAT AT^TAAT 1318 ATTAAT PvuII 362 CAGCTG CAG^CTG 2133 CAGCTG RcaI 227 TCATGA T^CATGA 980 TCATGA SphI 1115 GCATGC GCATG^C 1425 GCATGC SspI 667 AATATT AAT^ATT 1456 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BamHI BanI BanII BbvCI BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII