(Circular) (Six-base) MAPSORT of: pFN21AE4572.seq Check: 1945 from: 1 to: 6928 pFN21AE4572 6928 bp With 182 enzymes: SgfI * March 28, 2012 14:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2558 3946 2558 Size: 1388 5540 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4738 278 Size: 53 83 186 936 3202 2468 Fragments arranged by size: 3202 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4271 1937 Size: 2334 4594 Acc65I G'GTAC_C Cuts at: 4249 4249 Size: 6928 AclI AA'CG_TT Cuts at: 1563 5055 5428 1563 Size: 3492 373 3063 Fragments arranged by size: 3492 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 3726 4049 4120 4991 6375 6643 1632 Size: 638 1456 323 71 871 1384 268 1917 Fragments arranged by size: 1917 1456 1384 871 638 323 268 71 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6731 AflIII A'CryG_T Cuts at: 1203 2499 3727 5827 6804 1203 Size: 1296 1228 2100 977 1327 Fragments arranged by size: 2100 1327 1296 1228 977 AgeI A'CCGG_T Cuts at: 5759 5759 Size: 6928 AhdI GACnn_n'nnGTC Cuts at: 5657 5657 Size: 6928 AleI CACnn'nnGTG Cuts at: 1166 3730 1166 Size: 2564 4364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2153 1184 Size: 969 5959 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3497 3529 3497 Size: 32 6896 AlwNI CAG_nnn'CTG Cuts at: 2044 2155 3298 4671 6243 2044 Size: 111 1143 1373 1572 2729 Fragments arranged by size: 2729 1572 1373 1143 111 ApaI G_GGCC'C Cuts at: 3569 3569 Size: 6928 ApaLI G'TGCA_C Cuts at: 4985 6141 4985 Size: 1156 5772 ApoI r'AATT_y Cuts at: 1493 2655 2705 3158 3821 4237 4463 4615 6724 Size: 1162 50 453 663 416 226 152 2109 Cuts at: 6724 1493 Size: 1697 Fragments arranged by size: 2109 1697 1162 663 453 416 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 2400 3324 5482 160 Size: 872 1368 924 2158 1606 Fragments arranged by size: 2158 1606 1368 924 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 AvaI C'yCGr_G Cuts at: 1870 1943 3938 4253 1870 Size: 73 1995 315 4545 Fragments arranged by size: 4545 1995 315 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3472 3505 3472 Size: 33 6895 BamHI G'GATC_C Cuts at: 2543 2727 2751 4258 4625 2543 Size: 184 24 1507 367 4846 Fragments arranged by size: 4846 1507 367 184 24 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 4078 4148 4161 4249 Size: 325 217 627 549 1742 70 13 88 Cuts at: 4249 5704 618 Size: 1455 1842 Fragments arranged by size: 1842 1742 1455 627 549 325 217 88 70 13 BanII G_rGCy'C Cuts at: 1391 2428 2976 3048 3569 3939 4247 1391 Size: 1037 548 72 521 370 308 4072 Fragments arranged by size: 4072 1037 548 521 370 308 72 BbeI G_GCGC'C Cuts at: 4152 4152 Size: 6928 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5957 BbvCI CC'TCA_GC Cuts at: 2162 3228 3908 2162 Size: 1066 680 5182 Fragments arranged by size: 5182 1066 680 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2997 3031 5119 5153 1903 Size: 34 1060 34 2088 34 3678 Fragments arranged by size: 3678 2088 1060 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4820 6030 3500 Size: 1320 1210 4398 Fragments arranged by size: 4398 1320 1210 BclI T'GATC_A Cuts at: 1548 2635 1548 Size: 1087 5841 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5539 136 Size: 107 122 71 631 605 3867 1525 Fragments arranged by size: 3867 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3393 6923 3393 Size: 3530 3398 BlpI GC'TnA_GC Cuts at: 2970 3933 4345 2970 Size: 963 412 5553 Fragments arranged by size: 5553 963 412 Bme1580I G_kGCm'C Cuts at: 1790 3569 4083 4989 6145 1790 Size: 1779 514 906 1156 2573 Fragments arranged by size: 2573 1779 1156 906 514 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6928 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2945 3462 4659 Size: 416 53 83 399 24 1528 517 1197 Cuts at: 4659 5617 442 Size: 958 1753 Fragments arranged by size: 1753 1528 1197 958 517 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6928 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2247 3289 3433 5588 1239 Size: 1008 1042 144 2155 2579 Fragments arranged by size: 2579 2155 1042 1008 144 Bpu10I CC'TnA_GC Cuts at: 2162 3228 3908 2162 Size: 1066 680 5182 Fragments arranged by size: 5182 1066 680 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2411 2412 2960 5053 5918 6216 2411 Size: 1 548 2093 865 298 3123 Fragments arranged by size: 3123 2093 865 548 298 1 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5591 882 Size: 617 4092 2219 Fragments arranged by size: 4092 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3475 3828 4719 493 Size: 711 2271 353 891 2702 Fragments arranged by size: 2702 2271 891 711 353 BsaBI GATnn'nnATC Cuts at: 6922 6922 Size: 6928 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4149 4735 5117 5752 Size: 53 83 186 936 2616 586 382 635 Cuts at: 5752 275 Size: 1451 Fragments arranged by size: 2616 1451 936 635 586 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2339 2709 5361 5759 6033 6180 6591 2339 Size: 370 2652 398 274 147 411 2676 Fragments arranged by size: 2676 2652 411 398 370 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3287 3317 1178 Size: 30 165 30 1884 30 4789 Fragments arranged by size: 4789 1884 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6928 BseYI C'CCAG_C Cuts at: 2044 2375 3568 3690 6131 6627 2044 Size: 331 1193 122 2441 496 2345 Fragments arranged by size: 2441 2345 1193 496 331 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2078 4164 1163 Size: 573 342 2086 3927 Fragments arranged by size: 3927 2086 573 342 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4378 5139 6167 1540 Size: 275 186 2377 761 1028 2301 Fragments arranged by size: 2377 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2428 2976 3048 4989 5074 6145 2428 Size: 548 72 1941 85 1071 3211 Fragments arranged by size: 3211 1941 1071 548 85 72 BsmI GAATG_Cn' Cuts at: 4439 4532 4439 Size: 93 6835 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2428 2976 3048 3569 3939 4083 4247 Size: 399 638 548 72 521 370 144 164 Cuts at: 4247 4989 5074 6145 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 638 548 521 399 370 164 144 85 72 BspEI T'CCGG_A Cuts at: 6591 6591 Size: 6928 BspHI T'CATG_A Cuts at: 2232 2985 4817 6547 2232 Size: 753 1832 1730 2613 Fragments arranged by size: 2613 1832 1730 753 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2558 3516 3946 4052 4283 844 Size: 1714 958 430 106 231 3489 Fragments arranged by size: 3489 1714 958 430 231 106 BsrBI CCG'CTC Cuts at: 1394 1823 4815 1394 Size: 429 2992 3507 Fragments arranged by size: 3507 2992 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5423 5597 65 Size: 1183 4175 174 1396 Fragments arranged by size: 4175 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5572 5759 1700 Size: 110 3762 187 2869 Fragments arranged by size: 3762 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6928 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6928 BssSI C'ACGA_G Cuts at: 4988 6000 4988 Size: 1012 5916 BstAPI GCAn_nnn'nTGC Cuts at: 794 3176 4221 794 Size: 2382 1045 3501 Fragments arranged by size: 3501 2382 1045 BstBI TT'CG_AA Cuts at: 5732 5732 Size: 6928 BstEII G'GTnAC_C Cuts at: 1184 4039 4121 1184 Size: 2855 82 3991 Fragments arranged by size: 3991 2855 82 BstXI CCAn_nnnn'nTGG Cuts at: 3732 3732 Size: 6928 BstYI r'GATC_y Cuts at: 1972 2151 2512 2543 2727 2751 3393 4258 4625 Size: 179 361 31 184 24 642 865 367 Cuts at: 4625 5010 5027 6479 6565 6577 6923 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 865 642 385 367 361 346 184 179 86 31 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2021 3484 3883 513 Size: 552 939 17 1463 399 3558 Fragments arranged by size: 3558 1463 939 552 399 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6189 Fragments arranged by size: 6189 683 56 BtsI GCAGTG_nn' Cuts at: 3780 4444 5238 5258 3780 Size: 664 794 20 5450 Fragments arranged by size: 5450 794 664 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6928 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3878 3913 529 Size: 35 3314 35 3544 Fragments arranged by size: 3544 3314 35 35 DraI TTT'AAA Cuts at: 3789 4232 3789 Size: 443 6485 DraIII CAC_nnn'GTG Cuts at: 2022 4111 2022 Size: 2089 4839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5935 809 Size: 507 4619 1802 Fragments arranged by size: 4619 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3722 3899 4062 Size: 54 1425 50 275 18 1892 177 163 Cuts at: 4062 4375 5264 6893 8 Size: 313 889 1629 43 Fragments arranged by size: 1892 1629 1425 889 313 275 177 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4375 1537 Size: 275 2563 4090 Fragments arranged by size: 4090 2563 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 4139 4858 1353 Size: 665 2121 719 3423 Fragments arranged by size: 3423 2121 719 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2666 5515 5889 6035 387 Size: 1064 138 1077 2849 374 146 1280 Fragments arranged by size: 2849 1280 1077 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2426 2974 3046 2426 Size: 548 72 6308 Fragments arranged by size: 6308 548 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2247 2270 3289 3433 3726 4049 4120 Size: 393 615 23 1019 144 293 323 71 Cuts at: 4120 4991 5588 6375 6643 1239 Size: 871 597 787 268 1524 Fragments arranged by size: 1524 1019 871 787 615 597 393 323 293 268 144 71 23 EcoNI CCTnn'n_nnAGG Cuts at: 2573 2837 4010 2573 Size: 264 1173 5491 Fragments arranged by size: 5491 1173 264 EcoO109I rG'GnC_Cy Cuts at: 3505 3565 3505 Size: 60 6868 EcoRI G'AATT_C Cuts at: 2705 4237 4615 2705 Size: 1532 378 5018 Fragments arranged by size: 5018 1532 378 EcoRV GAT'ATC Cuts at: 2413 3313 2413 Size: 900 6028 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2065 3415 2065 Size: 1350 5578 FspI TGC'GCA Cuts at: 5434 5434 Size: 6928 HaeII r_GCGC'y Cuts at: 1678 3915 4152 6075 1678 Size: 2237 237 1923 2531 Fragments arranged by size: 2531 2237 1923 237 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2712 2744 3671 3703 5569 Size: 32 135 32 1140 32 927 32 1866 Cuts at: 5569 5601 5643 5675 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1866 1140 927 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3742 4272 4518 669 Size: 988 281 1804 530 246 3079 Fragments arranged by size: 3079 1804 988 530 281 246 HindIII A'AGCT_T Cuts at: 748 3926 748 Size: 3178 3750 HpaI GTT'AAC Cuts at: 4518 4518 Size: 6928 KasI G'GCGC_C Cuts at: 4148 4148 Size: 6928 KpnI G_GTAC'C Cuts at: 4253 4253 Size: 6928 MfeI C'AATT_G Cuts at: 4527 4527 Size: 6928 MluI A'CGCG_T Cuts at: 6804 6804 Size: 6928 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2283 3523 6042 6226 1305 Size: 978 1240 2519 184 2007 Fragments arranged by size: 2519 2007 1240 978 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3901 4064 10 Size: 54 1425 343 2069 163 2874 Fragments arranged by size: 2874 2069 1425 343 163 54 MslI CAynn'nnrTG Cuts at: 518 1166 3324 3419 3425 3730 4076 4886 5245 Size: 648 2158 95 6 305 346 810 359 Cuts at: 5245 5404 518 Size: 159 2042 Fragments arranged by size: 2158 2042 810 648 359 346 305 159 95 6 MspA1I CmG'CkG Cuts at: 1606 2369 4140 4344 5021 6169 6414 1606 Size: 763 1771 204 677 1148 245 2120 Fragments arranged by size: 2120 1771 1148 763 677 245 204 NarI GG'CG_CC Cuts at: 4149 4149 Size: 6928 NcoI C'CATG_G Cuts at: 513 1065 2004 3484 3883 513 Size: 552 939 1480 399 3558 Fragments arranged by size: 3558 1480 939 552 399 NdeI CA'TA_TG Cuts at: 387 3817 387 Size: 3430 3498 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6928 NotI GC'GGCC_GC Cuts at: 4375 4375 Size: 6928 NspI r_CATG'y Cuts at: 1132 1229 2503 3124 3434 3731 3814 4027 4286 Size: 97 1274 621 310 297 83 213 259 Cuts at: 4286 5831 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1274 621 310 297 259 213 97 83 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6928 PciI A'CATG_T Cuts at: 2499 3727 5827 2499 Size: 1228 2100 3600 Fragments arranged by size: 3600 2100 1228 PflMI CCAn_nnn'nTGG Cuts at: 1108 2225 1108 Size: 1117 5811 PfoI T'CCnGG_A Cuts at: 2154 2154 Size: 6928 PmeI GTTT'AAAC Cuts at: 4232 4232 Size: 6928 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4997 5029 6510 6542 4997 Size: 32 1481 32 5383 Fragments arranged by size: 5383 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3505 3505 Size: 6928 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6928 PsiI TTA'TAA Cuts at: 4498 4498 Size: 6928 PspOMI G'GGCC_C Cuts at: 3565 3565 Size: 6928 PstI C_TGCA'G Cuts at: 830 3513 4280 830 Size: 2683 767 3478 Fragments arranged by size: 3478 2683 767 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6928 PvuII CAG'CTG Cuts at: 2369 4140 2369 Size: 1771 5157 SacI G_AGCT'C Cuts at: 2428 2976 3048 2428 Size: 548 72 6308 Fragments arranged by size: 6308 548 72 SalI G'TCGA_C Cuts at: 1936 4270 1936 Size: 2334 4594 SapI GCTCTTCn'nnn_ Cuts at: 4139 4139 Size: 6928 SbfI CC_TGCA'GG Cuts at: 4280 4280 Size: 6928 ScaI AGT'ACT Cuts at: 5176 6888 5176 Size: 1712 5216 SfcI C'TryA_G Cuts at: 826 1045 2364 2680 3024 3509 3842 3917 4276 Size: 219 1319 316 344 485 333 75 359 Cuts at: 4276 5411 6092 6283 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1319 1135 681 485 359 344 333 316 219 191 75 SfoI GGC'GCC Cuts at: 4150 4150 Size: 6928 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6928 SmaI CCC'GGG Cuts at: 1872 3940 4255 1872 Size: 2068 315 4545 Fragments arranged by size: 4545 2068 315 SmlI C'TyrA_G Cuts at: 820 1017 1943 2390 2427 2975 5032 5933 6195 Size: 197 926 447 37 548 2057 901 262 Cuts at: 6195 820 Size: 1553 Fragments arranged by size: 2057 1553 926 901 548 447 262 197 37 SnaBI TAC'GTA Cuts at: 493 4719 493 Size: 4226 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6928 SphI G_CATG'C Cuts at: 1132 3124 3434 4286 1132 Size: 1992 310 852 3774 Fragments arranged by size: 3774 1992 852 310 SrfI GCCC'GGGC Cuts at: 3940 3940 Size: 6928 SspI AAT'ATT Cuts at: 5 52 2674 3463 4852 5 Size: 47 2622 789 1389 2081 Fragments arranged by size: 2622 2081 1389 789 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6928 StyI C'CwwG_G Cuts at: 513 1065 2004 2603 2838 3203 3484 3883 4065 Size: 552 939 599 235 365 281 399 182 Cuts at: 4065 4367 513 Size: 302 3074 Fragments arranged by size: 3074 939 599 552 399 365 302 281 235 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3643 4955 4972 5125 5310 5755 325 Size: 1255 2063 1312 17 153 185 445 1498 Fragments arranged by size: 2063 1498 1312 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2928 4032 5174 Size: 274 80 33 51 1443 951 1104 1142 Cuts at: 5174 6886 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1142 1104 951 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3287 3319 6888 6920 3287 Size: 32 3569 32 3295 Fragments arranged by size: 3569 3295 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6928 XbaI T'CTAG_A Cuts at: 4264 4264 Size: 6928 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4072 4072 Size: 6928 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6928 XmaI C'CCGG_G Cuts at: 1870 3938 4253 1870 Size: 2068 315 4545 Fragments arranged by size: 4545 2068 315 XmnI GAAnn'nnTTC Cuts at: 2898 5057 2898 Size: 2159 4769 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4736 276 Size: 53 83 186 936 3202 2468 Fragments arranged by size: 3202 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SwaI