Restriction map for
pFN21AE6648
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AseI
AsnI AspI AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HinfI HpaI Ksp632I
KspI MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 283 GGTACC G^GTACC
AfaI 284 GTAC GT^AC
AhdI 159 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 300 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 431 GGGCCC GGGCC^C
AscI 520 GGCGCGCC GG^CGCGCC
Asp700I 477 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 283 GGTACC G^GTACC
AspEI 159 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 379 GAGCAC G[AT]GC[AT]^C
AvaI 230 CCCGGG C^[CT]CG[GA]G
AviII 550 TGCGCA TGC^GCA
BanII 431 GGGCCC G[GA]GC[CT]^C
BseRI 211 GAGGAG GAGGAG(10/8)
BsiHKAI 379 GAGCAC G[AT]GC[AT]^C
BsmI 364 GAATGC GAATGC(1/-1)
BsoBI 230 CCCGGG C^[CT]CG[GA]G
BsrFI 328 ACCGGC [GA]^CCGG[CT]
BssHII 521 GCGCGC G^CGCGC
BstF5I 579 GGATG GGATG(2/0)
Cfr10I 328 ACCGGC [GA]^CCGG[CT]
DdeI 128 CTCAG C^T[ACGT]AG
DpnII 54 GATC ^GATC
Eam1105I 159 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 638 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 116 CCAAGG C^C[AT][AT]GG
FokI 579 GGATG GGATG(9/13)
FspI 550 TGCGCA TGC^GCA
KpnI 283 GGTACC GGTAC^C
MboI 54 GATC ^GATC
MboII 425 TCTTC GAAGA(8/7)
NdeII 54 GATC ^GATC
NotI 258 GCGGCCGC GC^GGCCGC
RsaI 284 GTAC GT^AC
Sau3AI 54 GATC ^GATC
SmaI 230 CCCGGG CCC^GGG
StyI 116 CCAAGG C^C[AT][AT]GG
Tsp509I 61 AATT ^AATT
XmaCI 230 CCCGGG C^CCGGG
XmaI 230 CCCGGG C^CCGGG
XmnI 477 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AluI 73 AGCT AG^CT
301 AGCT
BbeI 370 GGCGCC GGCGC^C
632 GGCGCC
BsgI 436 CTGCAC GTGCAG(16/14)
559 CTGCAC
BsiEI 259 CGGCCG CG[GA][CT]^CG
330 CGGCCG
BspMI 286 ACCTGC ACCTGC(4/8)
416 GCAGGT
BstAPI 299 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
359 GCAGTGAATGC
DraII 430 GGGGCCC [GA]G^G[ACGT]CC[CT]
431 GGGCCCT
EaeI 259 CGGCCG [CT]^GGCC[GA]
330 CGGCCG
EagI 259 CGGCCG C^GGCCG
330 CGGCCG
EclXI 259 CGGCCG C^GGCCG
330 CGGCCG
Eco52I 259 CGGCCG C^GGCCG
330 CGGCCG
EcoO109I 430 GGGGCCC [GA]G^G[ACGT]CC[CT]
431 GGGCCCT
FauI 586 CCCGC CCCGC(4/6)
624 GCGGG
HgaI 456 GACGC GACGC(5/10)
250 GCGTC
KasI 370 GGCGCC G^GCGCC
632 GGCGCC
MspA1I 465 CCGCTG C[AC]G^C[GT]G
622 CAGCGG
NarI 370 GGCGCC GG^CGCC
632 GGCGCC
NlaIII 16 CATG CATG^
346 CATG
PstI 270 CTGCAG CTGCA^G
414 CTGCAG
SfcI 270 CTGCAG C^T[GA][CT]AG
414 CTGCAG
SfoI 370 GGCGCC GGC^GCC
632 GGCGCC
TspRI 360 CAGTG CA[GC]TG[ACGT][ACGT]^
439 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AcyI AvaII BanI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrBI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I EcoRII Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI MaeIII
MnlI MspI MvaI MvnI MwoI
NciI NlaIV Sau96I ScrFI SfaNI
TseI Tsp45I