Restriction map for
pFN21AE6648
(Circular) (Six-base) MAPSORT of: pFN21AE6648.seq Check: 5924 from: 1 to: 5374
pFN21AE6648 5374 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2412 2412
Size: 5374
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2288 2695 2288
Size: 407 4967
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 4821 5089 1632
Size: 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 2169 4103 2169
Size: 1934 3440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AlwNI CAG_nnn'CTG
Cuts at: 2310 3117 4689 2310
Size: 807 1572 2995
Fragments arranged by size:
2995 1572 807
ApaI G_GGCC'C
Cuts at: 2440 2440
Size: 5374
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2683 2909 3061 5170 1493
Size: 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 226 152
AscI GG'CGCG_CC
Cuts at: 2526 2526
Size: 5374
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2235 2699 1870
Size: 73 292 464 4545
Fragments arranged by size:
4545 464 292 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2272 2280 2305 2313 2272
Size: 8 25 8 5333
Fragments arranged by size:
5333 25 8 8
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2288 2375 2637 2695
Size: 325 217 627 229 272 87 262 58
Cuts at: 2695 4150 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 272 262 229 217
87 58
BanII G_rGCy'C
Cuts at: 1391 2440 2693 1391
Size: 1049 253 4072
Fragments arranged by size:
4072 1049 253
BbeI G_GCGC'C
Cuts at: 2379 2641 2379
Size: 262 5112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3565 3599 1903
Size: 34 1628 34 3678
Fragments arranged by size:
3678 1628 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3985 136
Size: 107 122 71 631 605 2313 1525
Fragments arranged by size:
2313 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2321 2440 3435 4591 1790
Size: 531 119 995 1156 2573
Fragments arranged by size:
2573 1156 995 531 119
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2199 3105 4063
Size: 416 53 83 399 24 782 906 958
Cuts at: 4063 442
Size: 1753
Fragments arranged by size:
1753 958 906 782 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2152 2402 2441 2609 4034 1239
Size: 793 120 250 39 168 1425 2579
Fragments arranged by size:
2579 1425 793 250 168 120 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4037 882
Size: 617 2538 2219
Fragments arranged by size:
2538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3165 493
Size: 711 1961 2702
Fragments arranged by size:
2702 1961 711
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2376 2462 2638 3181
Size: 53 83 186 936 843 86 176 543
Cuts at: 3181 3563 4198 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 843 635 543 382 186 176
86 83 53
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 1178
Size: 30 165 30 747 30 4372
Fragments arranged by size:
4372 747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 1186
Size: 1045 4329
BseYI C'CCAG_C
Cuts at: 2205 2299 2531 4577 5073 2205
Size: 94 232 2046 496 2506
Fragments arranged by size:
2506 2046 496 232 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 2549 1163
Size: 573 690 123 3988
Fragments arranged by size:
3988 690 573 123
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2267 2338 2824 3585 4613 1540
Size: 275 186 266 71 486 761 1028 2301
Fragments arranged by size:
2301 1028 761 486 275 266 186 71
BsiHKAI G_wGCw'C
Cuts at: 2388 3435 3520 4591 2388
Size: 1047 85 1071 3171
Fragments arranged by size:
3171 1071 1047 85
BsmI GAATG_Cn'
Cuts at: 2375 2885 2978 2375
Size: 510 93 4771
Fragments arranged by size:
4771 510 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2321 2388 2440 2693 3435 3520 4591
Size: 399 531 67 52 253 742 85 1071
Cuts at: 4591 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 531 399 253 85 67
52
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2300 2412 2729 844
Size: 1456 112 317 3489
Fragments arranged by size:
3489 1456 317 112
BsrBI CCG'CTC
Cuts at: 1394 1823 2155 2224 2635 3261 1394
Size: 429 332 69 411 626 3507
Fragments arranged by size:
3507 626 429 411 332 69
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2333 4018 4205 1700
Size: 110 523 1685 187 2869
Fragments arranged by size:
2869 1685 523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 2526 1925
Size: 601 4773
BssSI C'ACGA_G
Cuts at: 3434 4446 3434
Size: 1012 4362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2310 2370 794
Size: 1516 60 3798
Fragments arranged by size:
3798 1516 60
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369
Size: 732 367 385 17 1452 86 12 346
Cuts at: 5369 1972
Size: 1977
Fragments arranged by size:
1977 1452 732 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4822
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2651 487
Size: 683 56 1425 3210
Fragments arranged by size:
3210 1425 683 56
BtsI GCAGTG_nn'
Cuts at: 2371 2443 2890 3684 3704 2371
Size: 72 447 794 20 4041
Fragments arranged by size:
4041 794 447 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2264 2335 2821
Size: 54 1425 50 275 18 434 71 486
Cuts at: 2821 3710 5339 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 486 434 275 71 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2264 2335 2821 1537
Size: 275 452 71 486 4090
Fragments arranged by size:
4090 486 452 275 71
EarI CTCTTCn'nnn_
Cuts at: 1353 3304 1353
Size: 1951 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2152 2402 2441 2609 3437 4034
Size: 393 400 120 250 39 168 828 597
Cuts at: 4034 4821 5089 1239
Size: 787 268 1524
Fragments arranged by size:
1524 828 787 597 400 393 268 250
168 120 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2647 2647
Size: 5374
EcoO109I rG'GnC_Cy
Cuts at: 2436 2437 2436
Size: 1 5373
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FspI TGC'GCA
Cuts at: 2557 3880 2557
Size: 1323 4051
HaeII r_GCGC'y
Cuts at: 1678 2199 2379 2520 2641 4521 1678
Size: 521 180 141 121 1880 2531
Fragments arranged by size:
2531 1880 521 180 141 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2132 2155 2187 2240
Size: 32 135 32 528 32 23 32 53
Cuts at: 2240 2272 4015 4047 4089 4121 1373
Size: 32 1743 32 42 32 2626
Fragments arranged by size:
2626 1743 528 135 53 42 32 32
32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KasI G'GCGC_C
Cuts at: 2375 2637 2375
Size: 262 5112
KpnI G_GTAC'C
Cuts at: 2292 2699 2292
Size: 407 4967
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4488 4672 1305
Size: 3183 184 2007
Fragments arranged by size:
3183 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3552
Fragments arranged by size:
3552 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3332 3691 3850 518
Size: 648 2166 359 159 2042
Fragments arranged by size:
2166 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2472 2629 2790 3467 4615 4860 1606
Size: 866 157 161 677 1148 245 2120
Fragments arranged by size:
2120 1148 866 677 245 161 157
NarI GG'CG_CC
Cuts at: 2376 2638 2376
Size: 262 5112
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4822
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2264 2821 2264
Size: 557 4817
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5374
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4472
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PspOMI G'GGCC_C
Cuts at: 2436 2436
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2279 2423 2726 830
Size: 1449 144 303 3478
Fragments arranged by size:
3478 1449 303 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2275 2419 2722 3857 4538 4729 826
Size: 219 1230 144 303 1135 681 191 1471
Fragments arranged by size:
1471 1230 1135 681 303 219 191 144
SfoI GGC'GCC
Cuts at: 2377 2639 2377
Size: 262 5112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2237 2701 1872
Size: 365 464 4545
Fragments arranged by size:
4545 464 365
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4379 4641 820
Size: 197 926 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2121 2813 513
Size: 552 1056 692 3074
Fragments arranged by size:
3074 1056 692 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2592 3401 3418 3571 3756 4201 325
Size: 1255 1012 809 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1012 809 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3620 5332 96
Size: 274 80 33 51 1443 1643 1712 138
Fragments arranged by size:
1712 1643 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2588 2620 5334 5366 2588
Size: 32 2714 32 2596
Fragments arranged by size:
2714 2596 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5374
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2235 2699 1870
Size: 365 464 4545
Fragments arranged by size:
4545 464 365
XmnI GAAnn'nnTTC
Cuts at: 2486 3503 2486
Size: 1017 4357
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI