Restriction map for
pFN21AB9861
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbeI BbrPI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspEI BspHI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CpoI DraI DraIII Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV FbaI FseI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp45I TspRI
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflIII 566 ACATGT A^C[GA][CT]GT
AlwI 501 GATCC GGATC(4/5)
AlwNI 1013 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 710 GGGCCC GGGCC^C
Asp700I 600 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 214 GAGCAC G[AT]GC[AT]^C
AviII 1020 TGCGCA TGC^GCA
BalI 353 TGGCCA TGG^CCA
BbvCI 910 CCTCAGC CCTCAGC(-5/-2)
BcgI 792 GCATCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 151 CTAG C^TAG
BsiHKAI 214 GAGCAC G[AT]GC[AT]^C
BsmAI 765 GAGAC GTCTC(1/5)
BsmBI 765 GAGACG CGTCTC(1/5)
BspDI 79 ATCGAT AT^CGAT
BspLU11I 566 ACATGT A^CATGT
BsrBI 1004 GAGCGG CCGCTC(-3/-3)
BsrFI 312 GCCGGC [GA]^CCGG[CT]
BstYI 500 AGATCC [GA]^GATC[CT]
Cfr10I 312 GCCGGC [GA]^CCGG[CT]
ClaI 79 ATCGAT AT^CGAT
DpnII 501 GATC ^GATC
DrdI 487 GACTGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 350 CCATGG C^C[GA][CT]GG
EagI 1179 CGGCCG C^GGCCG
EclXI 1179 CGGCCG C^GGCCG
Eco52I 1179 CGGCCG C^GGCCG
EcoNI 87 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 960 ATGCAT ATGCA^T
FspI 1020 TGCGCA TGC^GCA
HinfI 55 GACTC G^A[ACGT]TC
HphI 347 TCACC GGTGA(8/7)
MaeI 151 CTAG C^TAG
MboI 501 GATC ^GATC
MflI 500 AGATCC [GA]^GATC[CT]
MluNI 353 TGGCCA TGG^CCA
MscI 353 TGGCCA TGG^CCA
MslI 345 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 390 CAGCGG C[AC]G^C[GT]G
NaeI 312 GCCGGC GCC^GGC
NcoI 350 CCATGG C^CATGG
NdeII 501 GATC ^GATC
NgoMIV 312 GCCGGC G^CCGGC
NsiI 960 ATGCAT ATGCA^T
NspI 566 ACATGT [GA]CATG^[CT]
PleI 55 GACTC GAGTC(4/5)
Sau3AI 501 GATC ^GATC
Tsp509I 1090 AATT ^AATT
XhoII 500 AGATCC [GA]^GATC[CT]
XmnI 600 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcyI 767 GACGCC G[GA]^CG[CT]C
939 GGCGTC
AfaI 333 GTAC GT^AC
1010 GTAC
BglI 518 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1181 GCCGCGTCGGC
BlpI 16 GCTGAGC GC^T[ACGT]AGC
387 GCTCAGC
Bpu1102I 16 GCTGAGC GC^T[ACGT]AGC
387 GCTCAGC
BsaHI 767 GACGCC G[GA]^CG[CT]C
939 GGCGTC
BsrI 539 ACTGG ACTGG(1/-1)
730 CCAGT
CelII 16 GCTGAGC GC^T[ACGT]AGC
387 GCTCAGC
Hin1I 767 GACGCC G[GA]^CG[CT]C
939 GGCGTC
PstI 175 CTGCAG CTGCA^G
892 CTGCAG
RsaI 333 GTAC GT^AC
1010 GTAC
SfcI 175 CTGCAG C^T[GA][CT]AG
892 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI SmaI
StyI TaqI TseI TthHB8I XmaCI
XmaI