Restriction map for
pFN21AB9861
(Circular) (Six-base) MAPSORT of: pFN21AB9861.seq Check: 3582 from: 1 to: 5929
pFN21AB9861 5929 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2173 2652 2173
Size: 479 5450
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3739 278
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3272 1937
Size: 1335 4594
Acc65I G'GTAC_C
Cuts at: 3250 3250
Size: 5929
AclI AA'CG_TT
Cuts at: 1563 4056 4429 1563
Size: 2493 373 3063
Fragments arranged by size:
3063 2493 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2322 3992 5376 5644 1632
Size: 690 1670 1384 268 1917
Fragments arranged by size:
1917 1670 1384 690 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5732
AflIII A'CryG_T
Cuts at: 1203 2571 4828 5805 1203
Size: 1368 2257 977 1327
Fragments arranged by size:
2257 1368 1327 977
AgeI A'CCGG_T
Cuts at: 4760 4760
Size: 5929
AhdI GACnn_n'nnGTC
Cuts at: 4658 4658
Size: 5929
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5929
AlwNI CAG_nnn'CTG
Cuts at: 3023 3672 5244 3023
Size: 649 1572 3708
Fragments arranged by size:
3708 1572 649
ApaI G_GGCC'C
Cuts at: 2719 2719
Size: 5929
ApaLI G'TGCA_C
Cuts at: 3986 5142 3986
Size: 1156 4773
ApoI r'AATT_y
Cuts at: 1493 3238 3464 3616 5725 1493
Size: 1745 226 152 2109 1697
Fragments arranged by size:
2109 1745 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4483 160
Size: 872 3451 1606
Fragments arranged by size:
3451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2239 2632 2740 2765 3254 1870
Size: 73 271 25 393 108 25 489 4545
Fragments arranged by size:
4545 489 393 271 108 73 25 25
BamHI G'GATC_C
Cuts at: 3259 3626 3259
Size: 367 5562
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2261 2275 2537 2842 2882
Size: 325 217 627 474 14 262 305 40
Cuts at: 2882 3175 3250 4705 618
Size: 293 75 1455 1842
Fragments arranged by size:
1842 1455 627 474 325 305 293 262
217 75 40 14
BanII G_rGCy'C
Cuts at: 1391 2641 2719 3228 3248 1391
Size: 1250 78 509 20 4072
Fragments arranged by size:
4072 1250 509 78 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 2118 2130 928
Size: 971 180 39 12 4727
Fragments arranged by size:
4727 971 180 39 12
BbvCI CC'TCA_GC
Cuts at: 2916 2916
Size: 5929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2786 2820 3208 3242 4120 4154 1903
Size: 34 849 34 388 34 878 34 3678
Fragments arranged by size:
3678 878 849 388 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3821 5031 3821
Size: 1210 4719
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5929
BfrBI ATG'CAT
Cuts at: 2967 2967
Size: 5929
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2529 3192 4540
Size: 107 122 71 631 605 857 663 1348
Cuts at: 4540 136
Size: 1525
Fragments arranged by size:
1525 1348 857 663 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5924 5924
Size: 5929
BlpI GC'TnA_GC
Cuts at: 2022 2393 3346 2022
Size: 371 953 4605
Fragments arranged by size:
4605 953 371
Bme1580I G_kGCm'C
Cuts at: 1790 2278 2540 2719 2845 3178 3990 5146 1790
Size: 488 262 179 126 333 812 1156 2573
Fragments arranged by size:
2573 1156 812 488 333 262 179 126
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5929
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 4618 442
Size: 416 53 83 399 24 2243 958 1753
Fragments arranged by size:
2243 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2566 3053 4589 1239
Size: 914 413 487 1536 2579
Fragments arranged by size:
2579 1536 914 487 413
Bpu10I CC'TnA_GC
Cuts at: 2916 2916
Size: 5929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4054 4919 5217 4054
Size: 865 298 4766
Fragments arranged by size:
4766 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4592 882
Size: 617 3093 2219
Fragments arranged by size:
3093 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3720 493
Size: 711 2516 2702
Fragments arranged by size:
2702 2516 711
BsaBI GATnn'nnATC
Cuts at: 5923 5923
Size: 5929
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2773 2945 3736 4118
Size: 53 83 186 936 1240 172 791 382
Cuts at: 4118 4753 275
Size: 635 1451
Fragments arranged by size:
1451 1240 936 791 635 382 186 172
83 53
BsaWI w'CCGG_w
Cuts at: 4362 4760 5034 5181 5592 4362
Size: 398 274 147 411 4699
Fragments arranged by size:
4699 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2546 2576 1178
Size: 30 165 30 1143 30 4531
Fragments arranged by size:
4531 1143 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2144 2147 2150 2793 2942 3138 1186
Size: 928 30 3 3 643 149 196 3977
Fragments arranged by size:
3977 928 643 196 149 30 3 3
BseYI C'CCAG_C
Cuts at: 3167 5132 5628 3167
Size: 1965 496 3468
Fragments arranged by size:
3468 1965 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2655 2669 2774 1163
Size: 573 919 14 105 4318
Fragments arranged by size:
4318 919 573 105 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3045 3077 3187 3379 4140 5168
Size: 275 186 1044 32 110 192 761 1028
Cuts at: 5168 1540
Size: 2301
Fragments arranged by size:
2301 1044 1028 761 275 192 186 110
32
BsiHKAI G_wGCw'C
Cuts at: 2223 3990 4075 5146 2223
Size: 1767 85 1071 3006
Fragments arranged by size:
3006 1767 1071 85
BsmI GAATG_Cn'
Cuts at: 3440 3533 3440
Size: 93 5836
BsmBI CGTCTCn'nnnn_
Cuts at: 2764 2764
Size: 5929
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2278 2540 2641 2719 2845 3178
Size: 399 433 55 262 101 78 126 333
Cuts at: 3178 3228 3248 3990 4075 5146 1391
Size: 50 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 333 262 126
101 85 78 55 50 20
BspEI T'CCGG_A
Cuts at: 5592 5592
Size: 5929
BspHI T'CATG_A
Cuts at: 3818 5548 3818
Size: 1730 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2019 2173 2652 2674 3284 844
Size: 1175 154 479 22 610 3489
Fragments arranged by size:
3489 1175 610 479 154 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3011 3816 1394
Size: 429 1188 805 3507
Fragments arranged by size:
3507 1188 805 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4424 4598 65
Size: 1183 3176 174 1396
Fragments arranged by size:
3176 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2317 4573 4760 1700
Size: 110 507 2256 187 2869
Fragments arranged by size:
2869 2256 507 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5929
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5929
BssSI C'ACGA_G
Cuts at: 3989 5001 3989
Size: 1012 4917
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5929
BstBI TT'CG_AA
Cuts at: 4733 4733
Size: 5929
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5929
BstYI r'GATC_y
Cuts at: 1972 2505 3259 3626 4011 4028 5480 5566 5578
Size: 533 754 367 385 17 1452 86 12
Cuts at: 5578 5924 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 754 533 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2355 513
Size: 552 1290 4087
Fragments arranged by size:
4087 1290 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2326 487
Size: 683 56 1100 4090
Fragments arranged by size:
4090 1100 683 56
BtsI GCAGTG_nn'
Cuts at: 3445 4239 4259 3445
Size: 794 20 5115
Fragments arranged by size:
5115 794 20
ClaI AT'CG_AT
Cuts at: 1554 2085 1554
Size: 531 5398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5894
DraI TTT'AAA
Cuts at: 3233 3233
Size: 5929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2498 4936 809
Size: 507 1182 2438 1802
Fragments arranged by size:
2438 1802 1182 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2419 3184
Size: 54 1425 50 275 18 528 61 765
Cuts at: 3184 3376 4265 5894 8
Size: 192 889 1629 43
Fragments arranged by size:
1629 1425 889 765 528 275 192 61
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 3376 1537
Size: 275 1372 192 4090
Fragments arranged by size:
4090 1372 275 192
EarI CTCTTCn'nnn_
Cuts at: 1353 3859 1353
Size: 2506 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2459 2976 4516 4890 5036 387
Size: 1064 138 870 517 1540 374 146 1280
Fragments arranged by size:
1540 1280 1064 870 517 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2322 2566 3053 3992 4589 5376
Size: 393 521 169 244 487 939 597 787
Cuts at: 5376 5644 1239
Size: 268 1524
Fragments arranged by size:
1524 939 787 597 521 487 393 268
244 169
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2096
Size: 5929
EcoO109I rG'GnC_Cy
Cuts at: 2702 2715 2716 2745 2702
Size: 13 1 29 5886
Fragments arranged by size:
5886 29 13 1
EcoRI G'AATT_C
Cuts at: 3238 3616 3238
Size: 378 5551
FspI TGC'GCA
Cuts at: 3027 4435 3027
Size: 1408 4521
HaeII r_GCGC'y
Cuts at: 1678 2029 2386 2620 3198 5076 1678
Size: 351 357 234 578 1878 2531
Fragments arranged by size:
2531 1878 578 357 351 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2208 2240 2546 2578 4570
Size: 32 135 32 636 32 306 32 1992
Cuts at: 4570 4602 4644 4676 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1992 636 306 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3273 3519 669
Size: 988 281 1335 246 3079
Fragments arranged by size:
3079 1335 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5929
HpaI GTT'AAC
Cuts at: 3519 3519
Size: 5929
KpnI G_GTAC'C
Cuts at: 3254 3254
Size: 5929
MfeI C'AATT_G
Cuts at: 3528 3528
Size: 5929
MluI A'CGCG_T
Cuts at: 5805 5805
Size: 5929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3069 5043 5227 1305
Size: 1764 1974 184 2007
Fragments arranged by size:
2007 1974 1764 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 3579
Fragments arranged by size:
3579 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 3887 4246 4405 518
Size: 648 1188 1533 359 159 2042
Fragments arranged by size:
2042 1533 1188 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2397 3345 4022 5170 5415 1606
Size: 791 948 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 791 677 245
NaeI GCC'GGC
Cuts at: 2319 2319
Size: 5929
NcoI C'CATG_G
Cuts at: 513 1065 2355 513
Size: 552 1290 4087
Fragments arranged by size:
4087 1290 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5929
NgoMIV G'CCGG_C
Cuts at: 2317 2317
Size: 5929
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5929
NotI GC'GGCC_GC
Cuts at: 3376 3376
Size: 5929
NsiI A_TGCA'T
Cuts at: 2969 2969
Size: 5929
NspI r_CATG'y
Cuts at: 1132 1229 2575 3287 4832 1132
Size: 97 1346 712 1545 2229
Fragments arranged by size:
2229 1545 1346 712 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5929
PciI A'CATG_T
Cuts at: 2571 4828 2571
Size: 2257 3672
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5929
PfoI T'CCnGG_A
Cuts at: 2055 2055
Size: 5929
PmeI GTTT'AAAC
Cuts at: 3233 3233
Size: 5929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 5511 5543 3998
Size: 32 1481 32 4384
Fragments arranged by size:
4384 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5929
PsiI TTA'TAA
Cuts at: 3499 3499
Size: 5929
PspOMI G'GGCC_C
Cuts at: 2715 2715
Size: 5929
PstI C_TGCA'G
Cuts at: 830 2184 2901 3281 830
Size: 1354 717 380 3478
Fragments arranged by size:
3478 1354 717 380
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5929
SalI G'TCGA_C
Cuts at: 1936 3271 1936
Size: 1335 4594
SbfI CC_TGCA'GG
Cuts at: 3281 3281
Size: 5929
ScaI AGT'ACT
Cuts at: 4177 5889 4177
Size: 1712 4217
SfcI C'TryA_G
Cuts at: 826 1045 2180 2897 3277 4412 5093 5284 826
Size: 219 1135 717 380 1135 681 191 1471
Fragments arranged by size:
1471 1135 1135 717 681 380 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5929
SmaI CCC'GGG
Cuts at: 1872 2634 2742 2767 3256 1872
Size: 762 108 25 489 4545
Fragments arranged by size:
4545 762 489 108 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4033 4934 5196 820
Size: 197 926 2090 901 262 1553
Fragments arranged by size:
2090 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3720 493
Size: 3227 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5929
SphI G_CATG'C
Cuts at: 1132 3287 1132
Size: 2155 3774
SspI AAT'ATT
Cuts at: 5 52 3853 5
Size: 47 3801 2081
Fragments arranged by size:
3801 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5929
StyI C'CwwG_G
Cuts at: 513 1065 2282 2355 2921 3154 3368 513
Size: 552 1217 73 566 233 214 3074
Fragments arranged by size:
3074 1217 566 552 233 214 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2541 3956 3973 4126 4311 4756 325
Size: 1255 961 1415 17 153 185 445 1498
Fragments arranged by size:
1498 1415 1255 961 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4175 5887 96
Size: 274 80 33 51 1443 2198 1712 138
Fragments arranged by size:
2198 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5889 5921 5889
Size: 32 5897
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5929
XbaI T'CTAG_A
Cuts at: 3265 3265
Size: 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5929
XmaI C'CCGG_G
Cuts at: 1870 2632 2740 2765 3254 1870
Size: 762 108 25 489 4545
Fragments arranged by size:
4545 762 489 108 25
XmnI GAAnn'nnTTC
Cuts at: 2609 4058 2609
Size: 1449 4480
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3737 276
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI