Restriction map for
pFN21AB9861
(Linear) (Six-base) MAPSORT of: KIBB9861.seq Check: 7285 from: 1 to: 1224
KIBB9861.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 168 647 1224
Size: 168 479 577
Fragments arranged by size:
577 479 168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1224
Size: 317 907
AflIII A'CryG_T
Cuts at: 0 566 1224
Size: 566 658
AlwNI CAG_nnn'CTG
Cuts at: 0 1018 1224
Size: 1018 206
ApaI G_GGCC'C
Cuts at: 0 714 1224
Size: 714 510
AvaI C'yCGr_G
Cuts at: 0 209 234 627 735 760 1224
Size: 209 25 393 108 25 464
Fragments arranged by size:
464 393 209 108 25 25
BanI G'GyrC_C
Cuts at: 0 256 270 532 837 877 1170 1224
Size: 256 14 262 305 40 293 54
Fragments arranged by size:
305 293 262 256 54 40 14
BanII G_rGCy'C
Cuts at: 0 636 714 1223 1224
Size: 636 78 509 1
Fragments arranged by size:
636 509 78 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 113 125 1224
Size: 74 39 12 1099
Fragments arranged by size:
1099 74 39 12
BbvCI CC'TCA_GC
Cuts at: 0 911 1224
Size: 911 313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 781 815 1224
Size: 781 34 409
Fragments arranged by size:
781 409 34
BfrBI ATG'CAT
Cuts at: 0 962 1224
Size: 962 262
BglI GCCn_nnn'nGGC
Cuts at: 0 524 1187 1224
Size: 524 663 37
Fragments arranged by size:
663 524 37
BlpI GC'TnA_GC
Cuts at: 0 17 388 1224
Size: 17 371 836
Fragments arranged by size:
836 371 17
Bme1580I G_kGCm'C
Cuts at: 0 273 535 714 840 1173 1224
Size: 273 262 179 126 333 51
Fragments arranged by size:
333 273 262 179 126 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 561 1048 1224
Size: 148 413 487 176
Fragments arranged by size:
487 413 176 148
Bpu10I CC'TnA_GC
Cuts at: 0 911 1224
Size: 911 313
BsaHI Gr'CG_yC
Cuts at: 0 768 940 1224
Size: 768 172 284
Fragments arranged by size:
768 284 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 1224
Size: 541 30 653
Fragments arranged by size:
653 541 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 139 142 145 788 937 1133 1224
Size: 109 30 3 3 643 149 196 91
Fragments arranged by size:
643 196 149 109 91 30 3 3
BseYI C'CCAG_C
Cuts at: 0 1162 1224
Size: 1162 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 664 769 1224
Size: 650 14 105 455
Fragments arranged by size:
650 455 105 14
BsiEI CG_ry'CG
Cuts at: 0 1040 1072 1182 1224
Size: 1040 32 110 42
Fragments arranged by size:
1040 110 42 32
BsiHKAI G_wGCw'C
Cuts at: 0 218 1224
Size: 218 1006
BsmBI CGTCTCn'nnnn_
Cuts at: 0 759 1224
Size: 759 465
Bsp1286I G_dGCh'C
Cuts at: 0 218 273 535 636 714 840 1173 1223
Size: 218 55 262 101 78 126 333 50
Cuts at: 1223 1224
Size: 1
Fragments arranged by size:
333 262 218 126 101 78 55 50
1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 14 168 647 669 1224
Size: 14 154 479 22 555
Fragments arranged by size:
555 479 154 22 14
BsrBI CCG'CTC
Cuts at: 0 1006 1224
Size: 1006 218
BsrFI r'CCGG_y
Cuts at: 0 312 1224
Size: 312 912
BstYI r'GATC_y
Cuts at: 0 500 1224
Size: 500 724
BtgI C'CryG_G
Cuts at: 0 350 1224
Size: 350 874
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 321 1224
Size: 321 903
ClaI AT'CG_AT
Cuts at: 0 80 1224
Size: 80 1144
DrdI GACnn_nn'nnGTC
Cuts at: 0 493 1224
Size: 493 731
EaeI y'GGCC_r
Cuts at: 0 353 414 1179 1224
Size: 353 61 765 45
Fragments arranged by size:
765 353 61 45
EagI C'GGCC_G
Cuts at: 0 1179 1224
Size: 1179 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 454 971 1224
Size: 454 517 253
Fragments arranged by size:
517 454 253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 317 561 1048 1224
Size: 148 169 244 487 176
Fragments arranged by size:
487 244 176 169 148
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 1224
Size: 91 1133
EcoO109I rG'GnC_Cy
Cuts at: 0 697 710 711 740 1224
Size: 697 13 1 29 484
Fragments arranged by size:
697 484 29 13 1
FspI TGC'GCA
Cuts at: 0 1022 1224
Size: 1022 202
HaeII r_GCGC'y
Cuts at: 0 24 381 615 1193 1224
Size: 24 357 234 578 31
Fragments arranged by size:
578 357 234 31 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 203 235 541 573 1224
Size: 203 32 306 32 651
Fragments arranged by size:
651 306 203 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 1224
Size: 1064 160
MscI TGG'CCA
Cuts at: 0 355 1224
Size: 355 869
MslI CAynn'nnrTG
Cuts at: 0 349 1224
Size: 349 875
MspA1I CmG'CkG
Cuts at: 0 392 1224
Size: 392 832
NaeI GCC'GGC
Cuts at: 0 314 1224
Size: 314 910
NcoI C'CATG_G
Cuts at: 0 350 1224
Size: 350 874
NgoMIV G'CCGG_C
Cuts at: 0 312 1224
Size: 312 912
NsiI A_TGCA'T
Cuts at: 0 964 1224
Size: 964 260
NspI r_CATG'y
Cuts at: 0 570 1224
Size: 570 654
PciI A'CATG_T
Cuts at: 0 566 1224
Size: 566 658
PfoI T'CCnGG_A
Cuts at: 0 50 1224
Size: 50 1174
PspOMI G'GGCC_C
Cuts at: 0 710 1224
Size: 710 514
PstI C_TGCA'G
Cuts at: 0 179 896 1224
Size: 179 717 328
Fragments arranged by size:
717 328 179
SfcI C'TryA_G
Cuts at: 0 175 892 1224
Size: 175 717 332
Fragments arranged by size:
717 332 175
SmaI CCC'GGG
Cuts at: 0 629 737 762 1224
Size: 629 108 25 462
Fragments arranged by size:
629 462 108 25
StyI C'CwwG_G
Cuts at: 0 277 350 916 1149 1224
Size: 277 73 566 233 75
Fragments arranged by size:
566 277 233 75 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 536 1224
Size: 536 688
XmaI C'CCGG_G
Cuts at: 0 627 735 760 1224
Size: 627 108 25 464
Fragments arranged by size:
627 464 108 25
XmnI GAAnn'nnTTC
Cuts at: 0 604 1224
Size: 604 620
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I BpmI
Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BstYI BtgI BtgZI
ClaI DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PciI PfoI PspOMI PstI
SfcI SmaI StyI TaqII XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BclI BglII BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI CspCI
DraI DraIII EarI EcoICRI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI ZraI