################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tsp45I TspRI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AlwNI 76 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 72 TGGCCA TGG^CCA BamHI 179 GGATCC G^GATCC BbvCI 419 CCTCAGC CCTCAGC(-5/-2) BcnI 372 CCCGG CC^[GC]GG BglII 122 AGATCT A^GATCT BpmI 241 CTGGAG CTGGAG(16/14) BsmAI 214 GAGAC GTCTC(1/5) BsmFI 163 GTCCC GGGAC(10/14) Bsp1407I 266 TGTACA T^GTACA BsrGI 266 TGTACA T^GTACA BsrI 391 CCAGT ACTGG(1/-1) BstF5I 246 GGATG GGATG(2/0) BstXI 167 CCATTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 74 GCCAGC GC[ACGT]^[ACGT]GC DsaI 14 CCATGG C^C[GA][CT]GG EaeI 72 TGGCCA [CT]^GGCC[GA] EcoNI 136 CCTAACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 246 GGATG GGATG(9/13) HapII 373 CCGG C^CGG HpaII 373 CCGG C^CGG MluNI 72 TGGCCA TGG^CCA MscI 72 TGGCCA TGG^CCA MseI 35 TTAA T^TAA MspI 373 CCGG C^CGG NciI 372 CCCGG CC^[GC]GG NcoI 14 CCATGG C^CATGG NspI 205 GCATGT [GA]CATG^[CT] PflMI 75 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 161 AGGTCCC [GA]G^G[AT]CC[CT] SfcI 235 CTACAG C^T[GA][CT]AG SmlI 334 CTTGAG C^T[CT][GA]AG SspBI 266 TGTACA T^GTACA Tru9I 35 TTAA T^TAA Tsp509I 33 AATT ^AATT Van91I 75 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AvaII 150 GGACC G^G[AT]CC 162 GGTCC BanII 379 GAGCCC G[GA]GC[CT]^C 415 GAGCCC BmyI 379 GAGCCC G[AGT]GC[ACT]^C 415 GAGCCC BsgI 126 CTGCAC GTGCAG(16/14) 113 GTGCAG Bsp1286I 379 GAGCCC G[AGT]GC[ACT]^C 415 GAGCCC DdeI 315 CTCAG C^T[ACGT]AG 420 CTCAG DraII 161 AGGTCCC [GA]G^G[ACGT]CC[CT] 225 GGGGCCT EarI 220 GAAGAG CTCTTC(1/4) 280 GAAGAG EcoO109I 161 AGGTCCC [GA]G^G[ACGT]CC[CT] 225 GGGGCCT Ksp632I 220 GAAGAG CTCTTC(1/4) 280 GAAGAG NlaIII 15 CATG CATG^ 206 CATG PvuII 76 CAGCTG CAG^CTG 238 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BsaJI BsiYI BslI BssKI BstNI BstYI Cfr13I DpnII EcoRII EcoT14I Fnu4HI HaeIII HphI ItaI MboI MboII MflI MnlI MspA1I MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI StyI TseI XhoII