(Circular) (Six-base) MAPSORT of: pFN21AE1636.seq Check: 5966 from: 1 to: 5161 pFN21AE1636 5161 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2971 278 Size: 53 83 186 936 1435 2468 Fragments arranged by size: 2468 1435 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2504 1937 Size: 567 4594 Acc65I G'GTAC_C Cuts at: 2482 2482 Size: 5161 AclI AA'CG_TT Cuts at: 1563 3288 3661 1563 Size: 1725 373 3063 Fragments arranged by size: 3063 1725 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2317 3224 4608 4876 1632 Size: 685 907 1384 268 1917 Fragments arranged by size: 1917 1384 907 685 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4964 AflIII A'CryG_T Cuts at: 1203 4060 5037 1203 Size: 2857 977 1327 Fragments arranged by size: 2857 1327 977 AgeI A'CCGG_T Cuts at: 3992 3992 Size: 5161 AhdI GACnn_n'nnGTC Cuts at: 3890 3890 Size: 5161 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5161 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2128 1184 Size: 944 4217 AlwNI CAG_nnn'CTG Cuts at: 2086 2904 4476 2086 Size: 818 1572 2771 Fragments arranged by size: 2771 1572 818 ApaLI G'TGCA_C Cuts at: 3218 4374 3218 Size: 1156 4005 ApoI r'AATT_y Cuts at: 1493 2470 2696 2848 4957 1493 Size: 977 226 152 2109 1697 Fragments arranged by size: 2109 1697 977 226 152 AseI AT'TA_AT Cuts at: 160 1032 3715 160 Size: 872 2683 1606 Fragments arranged by size: 2683 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5161 AvaI C'yCGr_G Cuts at: 1870 1943 2486 1870 Size: 73 543 4545 Fragments arranged by size: 4545 543 73 BamHI G'GATC_C Cuts at: 2184 2491 2858 2184 Size: 307 367 4487 Fragments arranged by size: 4487 367 307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2482 3937 618 Size: 325 217 627 695 1455 1842 Fragments arranged by size: 1842 1455 695 627 325 217 BanII G_rGCy'C Cuts at: 1391 2388 2424 2480 1391 Size: 997 36 56 4072 Fragments arranged by size: 4072 997 56 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4190 BbvCI CC'TCA_GC Cuts at: 2425 2425 Size: 5161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3352 3386 1903 Size: 34 1415 34 3678 Fragments arranged by size: 3678 1415 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3053 4263 3053 Size: 1210 3951 BclI T'GATC_A Cuts at: 1548 1548 Size: 5161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3772 136 Size: 107 122 71 631 605 2100 1525 Fragments arranged by size: 2100 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2127 5156 2127 Size: 3029 2132 BlpI GC'TnA_GC Cuts at: 2578 2578 Size: 5161 Bme1580I G_kGCm'C Cuts at: 1790 3222 4378 1790 Size: 1432 1156 2573 Fragments arranged by size: 2573 1432 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2892 3850 442 Size: 416 53 83 399 24 1475 958 1753 Fragments arranged by size: 1753 1475 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 3821 1239 Size: 1028 1554 2579 Fragments arranged by size: 2579 1554 1028 Bpu10I CC'TnA_GC Cuts at: 2320 2425 2320 Size: 105 5056 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2360 3286 4151 4449 2360 Size: 926 865 298 3072 Fragments arranged by size: 3072 926 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3824 882 Size: 617 2325 2219 Fragments arranged by size: 2325 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2952 493 Size: 711 1748 2702 Fragments arranged by size: 2702 1748 711 BsaBI GATnn'nnATC Cuts at: 5155 5155 Size: 5161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2968 3350 3985 275 Size: 53 83 186 936 1435 382 635 1451 Fragments arranged by size: 1451 1435 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3594 3992 4266 4413 4824 3594 Size: 398 274 147 411 3931 Fragments arranged by size: 3931 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2085 2115 1178 Size: 30 165 30 682 30 4224 Fragments arranged by size: 4224 682 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5161 BseYI C'CCAG_C Cuts at: 2388 2457 4364 4860 2388 Size: 69 1907 496 2689 Fragments arranged by size: 2689 1907 496 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2116 2139 1163 Size: 573 380 23 4185 Fragments arranged by size: 4185 573 380 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2611 3372 4400 1540 Size: 275 186 610 761 1028 2301 Fragments arranged by size: 2301 1028 761 610 275 186 BsiHKAI G_wGCw'C Cuts at: 3222 3307 4378 3222 Size: 85 1071 4005 Fragments arranged by size: 4005 1071 85 BsmI GAATG_Cn' Cuts at: 2672 2765 2672 Size: 93 5068 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2388 2424 2480 3222 3307 4378 1391 Size: 399 598 36 56 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 598 399 85 56 36 BspEI T'CCGG_A Cuts at: 4824 4824 Size: 5161 BspHI T'CATG_A Cuts at: 3050 4780 3050 Size: 1730 3431 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2516 844 Size: 1672 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3048 1394 Size: 429 1225 3507 Fragments arranged by size: 3507 1225 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3656 3830 65 Size: 1183 2408 174 1396 Fragments arranged by size: 2408 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3805 3992 1700 Size: 110 1995 187 2869 Fragments arranged by size: 2869 1995 187 110 BsrGI T'GTAC_A Cuts at: 96 2271 96 Size: 2175 2986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5161 BssSI C'ACGA_G Cuts at: 3221 4233 3221 Size: 1012 4149 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5161 BstBI TT'CG_AA Cuts at: 3965 3965 Size: 5161 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5161 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2179 Size: 5161 BstYI r'GATC_y Cuts at: 1972 2095 2127 2184 2491 2858 3243 3260 4712 Size: 123 32 57 307 367 385 17 1452 Cuts at: 4712 4798 4810 5156 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 307 123 86 57 32 17 12 BtgI C'CryG_G Cuts at: 513 1065 2019 513 Size: 552 954 3655 Fragments arranged by size: 3655 954 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4422 Fragments arranged by size: 4422 683 56 BtsI GCAGTG_nn' Cuts at: 2677 3471 3491 2677 Size: 794 20 4347 Fragments arranged by size: 4347 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5161 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5126 DraI TTT'AAA Cuts at: 2465 2465 Size: 5161 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4168 809 Size: 507 2852 1802 Fragments arranged by size: 2852 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 2608 3497 Size: 54 1425 50 275 18 247 531 889 Cuts at: 3497 5126 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 531 275 247 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2608 1537 Size: 275 796 4090 Fragments arranged by size: 4090 796 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2220 2280 3091 1353 Size: 867 60 811 3423 Fragments arranged by size: 3423 867 811 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2317 3748 4122 4268 387 Size: 1064 138 728 1431 374 146 1280 Fragments arranged by size: 1431 1280 1064 728 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2317 3224 3821 4608 4876 1239 Size: 393 635 50 907 597 787 268 1524 Fragments arranged by size: 1524 907 787 635 597 393 268 50 EcoNI CCTnn'n_nnAGG Cuts at: 2145 2145 Size: 5161 EcoO109I rG'GnC_Cy Cuts at: 2167 2231 2167 Size: 64 5097 EcoRI G'AATT_C Cuts at: 2470 2848 2470 Size: 378 4783 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2461 Size: 5161 FspI TGC'GCA Cuts at: 3667 3667 Size: 5161 HaeII r_GCGC'y Cuts at: 1678 4308 1678 Size: 2630 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3802 3834 3876 3908 1373 Size: 32 135 32 2230 32 42 32 2626 Fragments arranged by size: 2626 2230 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2505 2751 669 Size: 988 281 567 246 3079 Fragments arranged by size: 3079 988 567 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5161 HpaI GTT'AAC Cuts at: 2751 2751 Size: 5161 KpnI G_GTAC'C Cuts at: 2486 2486 Size: 5161 MfeI C'AATT_G Cuts at: 2760 2760 Size: 5161 MluI A'CGCG_T Cuts at: 5037 5037 Size: 5161 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4275 4459 1305 Size: 2970 184 2007 Fragments arranged by size: 2970 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2079 10 Size: 54 1425 343 247 3092 Fragments arranged by size: 3092 1425 343 247 54 MslI CAynn'nnrTG Cuts at: 518 1166 3119 3478 3637 518 Size: 648 1953 359 159 2042 Fragments arranged by size: 2042 1953 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2202 2245 2577 3254 4402 4647 1606 Size: 477 119 43 332 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 477 332 245 119 43 NcoI C'CATG_G Cuts at: 513 1065 2019 513 Size: 552 954 3655 Fragments arranged by size: 3655 954 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5161 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5161 NotI GC'GGCC_GC Cuts at: 2608 2608 Size: 5161 NspI r_CATG'y Cuts at: 1132 1229 2214 2519 4064 1132 Size: 97 985 305 1545 2229 Fragments arranged by size: 2229 1545 985 305 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5161 PciI A'CATG_T Cuts at: 4060 4060 Size: 5161 PflMI CCAn_nnn'nTGG Cuts at: 1108 2086 1108 Size: 978 4183 PmeI GTTT'AAAC Cuts at: 2465 2465 Size: 5161 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3230 3262 4743 4775 3230 Size: 32 1481 32 3616 Fragments arranged by size: 3616 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2167 2167 Size: 5161 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5161 PsiI TTA'TAA Cuts at: 2731 2731 Size: 5161 PstI C_TGCA'G Cuts at: 830 2513 830 Size: 1683 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5161 PvuII CAG'CTG Cuts at: 2083 2245 2083 Size: 162 4999 SalI G'TCGA_C Cuts at: 1936 2503 1936 Size: 567 4594 SbfI CC_TGCA'GG Cuts at: 2513 2513 Size: 5161 ScaI AGT'ACT Cuts at: 3409 5121 3409 Size: 1712 3449 SfcI C'TryA_G Cuts at: 826 1045 2240 2509 3644 4325 4516 826 Size: 219 1195 269 1135 681 191 1471 Fragments arranged by size: 1471 1195 1135 681 269 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5161 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5161 SmaI CCC'GGG Cuts at: 1872 2488 1872 Size: 616 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 3265 4166 4428 820 Size: 197 926 396 926 901 262 1553 Fragments arranged by size: 1553 926 926 901 396 262 197 SnaBI TAC'GTA Cuts at: 493 2952 493 Size: 2459 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5161 SphI G_CATG'C Cuts at: 1132 2519 1132 Size: 1387 3774 SspI AAT'ATT Cuts at: 5 52 3085 5 Size: 47 3033 2081 Fragments arranged by size: 3033 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5161 StyI C'CwwG_G Cuts at: 513 1065 2019 2163 2409 2600 513 Size: 552 954 144 246 191 3074 Fragments arranged by size: 3074 954 552 246 191 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3188 3205 3358 3543 3988 325 Size: 1255 1608 17 153 185 445 1498 Fragments arranged by size: 1608 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 2398 3407 Size: 274 80 33 51 1443 294 127 1009 Cuts at: 3407 5119 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1009 294 274 138 127 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5121 5153 5121 Size: 32 5129 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5161 XbaI T'CTAG_A Cuts at: 2497 2497 Size: 5161 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5161 XmaI C'CCGG_G Cuts at: 1870 2486 1870 Size: 616 4545 XmnI GAAnn'nnTTC Cuts at: 3290 3290 Size: 5161 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2969 276 Size: 53 83 186 936 1435 2468 Fragments arranged by size: 2468 1435 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI